Tags

Type your tag names separated by a space and hit enter

Evaluation of a Histoplasma antigen Lateral Flow Assay for the rapid diagnosis of progressive disseminated histoplasmosis in Colombian patients with AIDS.
Mycoses 2019M

Abstract

BACKGROUND

Progressive disseminated histoplasmosis (PDH) is an important cause of mortality in persons living with HIV (PLHIV), especially in countries where patients have limited access to antiretroviral therapies and diagnostic testing.

OBJECTIVE

A Lateral Flow Assay (LFA) to detect Histoplasma capsulatum antigen in serum developed by MiraVista® was evaluated.

METHODS

We tested 75 serum samples: 24 from PLHIV and culture-proven PDH and 51 from PLHIV with other fungal and bacterial infections as well as people without HIV. LFA devices were read manually (read by eye) and by an automated reader.

RESULTS

When the LFA was read manually, sensitivity was 96% and specificity was 90%. When an automated reader was used, sensitivity was 92% and specificity was 94%. The Kappa index comparing manual and automated reader was 0.90. Cross-reactions were observed principally in samples from patients with proven diagnosis of paracoccidioidomycosis.

CONCLUSIONS

The MiraVista® Diagnostics Histoplasma antigen LFA had high analytical performance and good agreement between manual and automated reader. This LFA allows Histoplasma antigen testing with minimal laboratory equipment and infrastructure requirements.

Authors+Show Affiliations

Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA. Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Bogota, Colombia.Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Bogota, Colombia.Hospital La Maria, Medellin, Colombia. Universidad CES, Medellin, Colombia.Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31625627

Citation

Cáceres, Diego H., et al. "Evaluation of a Histoplasma Antigen Lateral Flow Assay for the Rapid Diagnosis of Progressive Disseminated Histoplasmosis in Colombian Patients With AIDS." Mycoses, 2019.
Cáceres DH, Gómez BL, Tobón AM, et al. Evaluation of a Histoplasma antigen Lateral Flow Assay for the rapid diagnosis of progressive disseminated histoplasmosis in Colombian patients with AIDS. Mycoses. 2019.
Cáceres, D. H., Gómez, B. L., Tobón, A. M., Chiller, T. M., & Lindsley, M. D. (2019). Evaluation of a Histoplasma antigen Lateral Flow Assay for the rapid diagnosis of progressive disseminated histoplasmosis in Colombian patients with AIDS. Mycoses, doi:10.1111/myc.13023.
Cáceres DH, et al. Evaluation of a Histoplasma Antigen Lateral Flow Assay for the Rapid Diagnosis of Progressive Disseminated Histoplasmosis in Colombian Patients With AIDS. Mycoses. 2019 Oct 18; PubMed PMID: 31625627.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of a Histoplasma antigen Lateral Flow Assay for the rapid diagnosis of progressive disseminated histoplasmosis in Colombian patients with AIDS. AU - Cáceres,Diego H, AU - Gómez,Beatriz L, AU - Tobón,Angela M, AU - Chiller,Tom M, AU - Lindsley,Mark D, Y1 - 2019/10/18/ PY - 2019/10/19/entrez JF - Mycoses JO - Mycoses N2 - BACKGROUND: Progressive disseminated histoplasmosis (PDH) is an important cause of mortality in persons living with HIV (PLHIV), especially in countries where patients have limited access to antiretroviral therapies and diagnostic testing. OBJECTIVE: A Lateral Flow Assay (LFA) to detect Histoplasma capsulatum antigen in serum developed by MiraVista® was evaluated. METHODS: We tested 75 serum samples: 24 from PLHIV and culture-proven PDH and 51 from PLHIV with other fungal and bacterial infections as well as people without HIV. LFA devices were read manually (read by eye) and by an automated reader. RESULTS: When the LFA was read manually, sensitivity was 96% and specificity was 90%. When an automated reader was used, sensitivity was 92% and specificity was 94%. The Kappa index comparing manual and automated reader was 0.90. Cross-reactions were observed principally in samples from patients with proven diagnosis of paracoccidioidomycosis. CONCLUSIONS: The MiraVista® Diagnostics Histoplasma antigen LFA had high analytical performance and good agreement between manual and automated reader. This LFA allows Histoplasma antigen testing with minimal laboratory equipment and infrastructure requirements. SN - 1439-0507 UR - https://www.unboundmedicine.com/medline/citation/31625627/Evaluation_of_a_Histoplasma_antigen_Lateral_Flow_Assay_for_the_rapid_diagnosis_of_progressive_disseminated_histoplasmosis_in_Colombian_patients_with_AIDS L2 - https://doi.org/10.1111/myc.13023 DB - PRIME DP - Unbound Medicine ER -