Tags

Type your tag names separated by a space and hit enter

mcr-1 encoding colistin resistance in CTX-M-1/CTX-M-15- producing Escherichia coli isolates of bovine and caprine origins in Tunisia. First report of CTX-M-15-ST394/D E. coli from goats.

Abstract

The objective of this study was to isolate and characterize ESBL-producing Escherichia coli (ESBL-EC) from raw bovine and caprine milk samples, as well as from bovine faeces in Tunisia. Therefore, 120 bovine faecal samples and 9 caprine raw milk samples were collected from 2 extensive dairy-cow-farms and 5 ovine farms, respectively. In addition, 94 raw bovine milk samples, from containers and holding tanks from 50 small public-markets in the North of Tunisia, were processed for the isolation of cefotaxime-resistant E. coli (CTXR). Antimicrobial susceptibility testing was carried out by disc-diffusion/broth-microdilution methods. The presence of genes encoding ESBL, as well as those encoding colistin (mcr-1 to 5 genes)- sulfonamide-, tetracycline-, gentamicin-, quinolone and chloramphenicol-resistance and class 1 integrons were tested by PCR (and sequencing in some cases). ESBL-EC isolates were further characterized by phylogrouping and MLST/PFGE typing. Eight samples (3.6%) contained ESBL-EC isolates (3/2 from raw bovine/goat milk and 3 from cattle faeces) and one isolate/sample was characterized. Four ESBL-EC isolates, all of bovine origin (3 faeces/1 milk), were resistant to colistin (MIC: 8-16 μg/ml), harboured the mcr-1 gene and carried IncP- and IncFIB-type plasmids. The 8 ESBL-EC strains had the following characteristics: a) bovine faeces: mcr-1/CTX-M-1/D-ST1642 (3 strains); b) raw milk: mcr-1/CTX-M-1/A-ST10 (1 strain); CTX-M-15/B1-ST394 (3 strains), and CTX-M-15/A-ST46 (1 strain). Most of bovine ESBL-EC isolates were multidrug-resistant (4/5). Our results showed that ESBL-EC were detected in bovine and caprine samples (CTX-M-1/CTX-M-15 producers), being some of them colistin-resistant (associated with mcr-1 gene), and they belonged to international clonal lineages.

Authors+Show Affiliations

Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisia.Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisia.Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisia; Université de Tunis El Manar, Faculté de Médecine de Tunis, Laboratoire de résistance aux antibiotiques LR99ES09, Tunisia.Departamento de Agricultura y Alimentación, Universidad de La Rioja, 26006 Logroño, Spain.Departamento de Agricultura y Alimentación, Universidad de La Rioja, 26006 Logroño, Spain.Laboratoire de Traitement des Eaux Usées, Centre des Recherches et des Technologies des Eaux (CERTE), Technopole Borj-Cédria, BP 273, 8020, Soliman, Tunisia.Université de la Manouba, IRESA, École Nationale de Médecine Vétérinaire de Sidi Thabet, Sidi Thabet 2020, Sidi Thabet, Ariana, Tunisia.Departamento de Agricultura y Alimentación, Universidad de La Rioja, 26006 Logroño, Spain. Electronic address: carmen.torres@unirioja.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31627036

Citation

Hassen, Bilel, et al. "Mcr-1 Encoding Colistin Resistance in CTX-M-1/CTX-M-15- Producing Escherichia Coli Isolates of Bovine and Caprine Origins in Tunisia. First Report of CTX-M-15-ST394/D E. Coli From Goats." Comparative Immunology, Microbiology and Infectious Diseases, vol. 67, 2019, p. 101366.
Hassen B, Saloua B, Abbassi MS, et al. Mcr-1 encoding colistin resistance in CTX-M-1/CTX-M-15- producing Escherichia coli isolates of bovine and caprine origins in Tunisia. First report of CTX-M-15-ST394/D E. coli from goats. Comp Immunol Microbiol Infect Dis. 2019;67:101366.
Hassen, B., Saloua, B., Abbassi, M. S., Ruiz-Ripa, L., Mama, O. M., Hassen, A., ... Torres, C. (2019). Mcr-1 encoding colistin resistance in CTX-M-1/CTX-M-15- producing Escherichia coli isolates of bovine and caprine origins in Tunisia. First report of CTX-M-15-ST394/D E. coli from goats. Comparative Immunology, Microbiology and Infectious Diseases, 67, p. 101366. doi:10.1016/j.cimid.2019.101366.
Hassen B, et al. Mcr-1 Encoding Colistin Resistance in CTX-M-1/CTX-M-15- Producing Escherichia Coli Isolates of Bovine and Caprine Origins in Tunisia. First Report of CTX-M-15-ST394/D E. Coli From Goats. Comp Immunol Microbiol Infect Dis. 2019 Oct 4;67:101366. PubMed PMID: 31627036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - mcr-1 encoding colistin resistance in CTX-M-1/CTX-M-15- producing Escherichia coli isolates of bovine and caprine origins in Tunisia. First report of CTX-M-15-ST394/D E. coli from goats. AU - Hassen,Bilel, AU - Saloua,Benlabidi, AU - Abbassi,Mohamed Salah, AU - Ruiz-Ripa,Laura, AU - Mama,Olouwafemi M, AU - Hassen,Abdennaceur, AU - Hammami,Salah, AU - Torres,Carmen, Y1 - 2019/10/04/ PY - 2019/07/31/received PY - 2019/09/25/revised PY - 2019/09/29/accepted PY - 2019/10/19/pubmed PY - 2019/10/19/medline PY - 2019/10/19/entrez KW - Antibiotic resistance KW - Colistin KW - ESBL KW - Escherichia coli KW - Tunisia KW - mcr-1 SP - 101366 EP - 101366 JF - Comparative immunology, microbiology and infectious diseases JO - Comp. Immunol. Microbiol. Infect. Dis. VL - 67 N2 - The objective of this study was to isolate and characterize ESBL-producing Escherichia coli (ESBL-EC) from raw bovine and caprine milk samples, as well as from bovine faeces in Tunisia. Therefore, 120 bovine faecal samples and 9 caprine raw milk samples were collected from 2 extensive dairy-cow-farms and 5 ovine farms, respectively. In addition, 94 raw bovine milk samples, from containers and holding tanks from 50 small public-markets in the North of Tunisia, were processed for the isolation of cefotaxime-resistant E. coli (CTXR). Antimicrobial susceptibility testing was carried out by disc-diffusion/broth-microdilution methods. The presence of genes encoding ESBL, as well as those encoding colistin (mcr-1 to 5 genes)- sulfonamide-, tetracycline-, gentamicin-, quinolone and chloramphenicol-resistance and class 1 integrons were tested by PCR (and sequencing in some cases). ESBL-EC isolates were further characterized by phylogrouping and MLST/PFGE typing. Eight samples (3.6%) contained ESBL-EC isolates (3/2 from raw bovine/goat milk and 3 from cattle faeces) and one isolate/sample was characterized. Four ESBL-EC isolates, all of bovine origin (3 faeces/1 milk), were resistant to colistin (MIC: 8-16 μg/ml), harboured the mcr-1 gene and carried IncP- and IncFIB-type plasmids. The 8 ESBL-EC strains had the following characteristics: a) bovine faeces: mcr-1/CTX-M-1/D-ST1642 (3 strains); b) raw milk: mcr-1/CTX-M-1/A-ST10 (1 strain); CTX-M-15/B1-ST394 (3 strains), and CTX-M-15/A-ST46 (1 strain). Most of bovine ESBL-EC isolates were multidrug-resistant (4/5). Our results showed that ESBL-EC were detected in bovine and caprine samples (CTX-M-1/CTX-M-15 producers), being some of them colistin-resistant (associated with mcr-1 gene), and they belonged to international clonal lineages. SN - 1878-1667 UR - https://www.unboundmedicine.com/medline/citation/31627036/mcr-1_encoding_colistin_resistance_in_CTX-M-1/CTX-M-15-_producing_Escherichia_coli_isolates_of_bovine_and_caprine_origins_in_Tunisia._First_report_of_CTX-M-15-ST394/D_E._coli_from_goats L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-9571(19)30163-8 DB - PRIME DP - Unbound Medicine ER -