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Cholesterol, a powerful 13C isotopic biomarker.
Anal Chim Acta 2019; 1089:115-122AC

Abstract

Cholesterol is related to many health diseases and is considered as a metabolic disorder biomarker. This compound, present in all food products of animal origin, can also be used as food authentication biomarker. In this work and for the first time, positional 13C isotope contents were determined for such a high molecular weight compound. This was possible by means of NMR using adiabatic refocused INEPT. In order to test the potential of this approach for discrimination, hen eggs from different origins were collected. Quantitative extraction of egg yolk cholesterol was optimized, and partial reduced molar fractions of its different 13C isotopomers were used as predictors in discriminant analysis. Compared with the global 13C isotopic composition determined using isotope ratio monitoring by Mass Spectrometry, the relative content of cholesterol 13C isotopomers added valuable power to sample classifications according to their origins. This study paves the way to isotopomics of other steroids and similar molecular weight compounds.

Authors+Show Affiliations

Laboratory of Metrology and Isotopic Fractionation, Research Unit: Technologies et Valorisation Agroalimentaire (TVA), Faculty of Science, Saint Joseph University of Beirut, P.O. Box 17-5208, Mar Mikhael, Beirut, 1104 2020, Lebanon; EBSI Team, Interdisciplinary Chemistry: Synthesis, Analysis, Modelling (CEISAM), University of Nantes-CNRS UMR 6230, 2 Rue de La Houssinière, BP 92208, F-44322, Nantes Cedex 3, France.Laboratory of Metrology and Isotopic Fractionation, Research Unit: Technologies et Valorisation Agroalimentaire (TVA), Faculty of Science, Saint Joseph University of Beirut, P.O. Box 17-5208, Mar Mikhael, Beirut, 1104 2020, Lebanon.EBSI Team, Interdisciplinary Chemistry: Synthesis, Analysis, Modelling (CEISAM), University of Nantes-CNRS UMR 6230, 2 Rue de La Houssinière, BP 92208, F-44322, Nantes Cedex 3, France.Laboratory of Metrology and Isotopic Fractionation, Research Unit: Technologies et Valorisation Agroalimentaire (TVA), Faculty of Science, Saint Joseph University of Beirut, P.O. Box 17-5208, Mar Mikhael, Beirut, 1104 2020, Lebanon. Electronic address: joseph.bejjani@usj.edu.lb.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31627808

Citation

Hajjar, Ghina, et al. "Cholesterol, a Powerful 13C Isotopic Biomarker." Analytica Chimica Acta, vol. 1089, 2019, pp. 115-122.
Hajjar G, Rizk T, Akoka S, et al. Cholesterol, a powerful 13C isotopic biomarker. Anal Chim Acta. 2019;1089:115-122.
Hajjar, G., Rizk, T., Akoka, S., & Bejjani, J. (2019). Cholesterol, a powerful 13C isotopic biomarker. Analytica Chimica Acta, 1089, pp. 115-122. doi:10.1016/j.aca.2019.09.001.
Hajjar G, et al. Cholesterol, a Powerful 13C Isotopic Biomarker. Anal Chim Acta. 2019 Dec 16;1089:115-122. PubMed PMID: 31627808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cholesterol, a powerful 13C isotopic biomarker. AU - Hajjar,Ghina, AU - Rizk,Toufic, AU - Akoka,Serge, AU - Bejjani,Joseph, Y1 - 2019/09/03/ PY - 2019/06/12/received PY - 2019/08/08/revised PY - 2019/09/01/accepted PY - 2019/10/20/entrez KW - Adiabatic refocused INEPT KW - Cholesterol KW - Irm-MS KW - Isotopic biomarkers KW - Position-specific isotopic analysis (PSIA) KW - Quantitative (13)C NMR SP - 115 EP - 122 JF - Analytica chimica acta JO - Anal. Chim. Acta VL - 1089 N2 - Cholesterol is related to many health diseases and is considered as a metabolic disorder biomarker. This compound, present in all food products of animal origin, can also be used as food authentication biomarker. In this work and for the first time, positional 13C isotope contents were determined for such a high molecular weight compound. This was possible by means of NMR using adiabatic refocused INEPT. In order to test the potential of this approach for discrimination, hen eggs from different origins were collected. Quantitative extraction of egg yolk cholesterol was optimized, and partial reduced molar fractions of its different 13C isotopomers were used as predictors in discriminant analysis. Compared with the global 13C isotopic composition determined using isotope ratio monitoring by Mass Spectrometry, the relative content of cholesterol 13C isotopomers added valuable power to sample classifications according to their origins. This study paves the way to isotopomics of other steroids and similar molecular weight compounds. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/31627808/Cholesterol,_a_powerful_13C_isotopic_biomarker L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(19)31044-X DB - PRIME DP - Unbound Medicine ER -