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A distinct double positive IL-17A+/F+ T helper 17 cells induced inflammation leads to IL17 producing neutrophils in Type 1 reaction of leprosy patients.
Cytokine 2019; 126:154873C

Abstract

Type 1 reactions (T1R) an inflammatory condition, of local skin patches in 30-40% leprosy patients during the course of MDT. IL-17A and IL-17F play an important role in regulating skin inflammation through neutrophils. In the present study, we have analyzed 18 of each T1R and Non-reactions (NR) patients through flow cytometry and qPCR. Interestingly we found that, CD3+CD4+ gated IL-17A+IL-17F+ cells were significantly high in T1R in both MLSA stimulated PBMCs and skin lesions as compared to NR leprosy patients. Hierarchical clustering analysis of gene expression showed that CXCL6, CXCL5, CCL20, CCL7, MMP13 and IL-17RB expression were significantly associated with IL-17A and IL-17F expression (Spearman r2 = 0.77 to 0.98), neutrophils and monocyte markers respectively. In this study, the inflammation noted in lesions of T1R is a different phenotype of Th17 which produce double positive IL-17A+IL17F+ and also contributes IL-17 producing neutrophils and thus would be useful for monitoring, diagnosis and treatment response before reactions episodes.

Authors+Show Affiliations

Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India; Department of Biotechnology, All India Institute of Medical Sciences, Safdarjung Hospital, Ansari Nagar, New Delhi 110029, India. Electronic address: chamansaini82@gmail.com.Department of Biotechnology, All India Institute of Medical Sciences, Safdarjung Hospital, Ansari Nagar, New Delhi 110029, India.Department of Microbiology, Government Medical College, Kota 324005, India.Department of Dermatology, Safdarjung Hospital, Ansari Nagar, New Delhi 110029, India.Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India. Electronic address: dralpanasharma@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31629113

Citation

Saini, Chaman, et al. "A Distinct Double Positive IL-17A+/F+ T Helper 17 Cells Induced Inflammation Leads to IL17 Producing Neutrophils in Type 1 Reaction of Leprosy Patients." Cytokine, vol. 126, 2019, p. 154873.
Saini C, Srivastava RK, Kumar P, et al. A distinct double positive IL-17A+/F+ T helper 17 cells induced inflammation leads to IL17 producing neutrophils in Type 1 reaction of leprosy patients. Cytokine. 2019;126:154873.
Saini, C., Srivastava, R. K., Kumar, P., Ramesh, V., & Sharma, A. (2019). A distinct double positive IL-17A+/F+ T helper 17 cells induced inflammation leads to IL17 producing neutrophils in Type 1 reaction of leprosy patients. Cytokine, 126, p. 154873. doi:10.1016/j.cyto.2019.154873.
Saini C, et al. A Distinct Double Positive IL-17A+/F+ T Helper 17 Cells Induced Inflammation Leads to IL17 Producing Neutrophils in Type 1 Reaction of Leprosy Patients. Cytokine. 2019 Oct 16;126:154873. PubMed PMID: 31629113.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A distinct double positive IL-17A+/F+ T helper 17 cells induced inflammation leads to IL17 producing neutrophils in Type 1 reaction of leprosy patients. AU - Saini,Chaman, AU - Srivastava,Rupesh K, AU - Kumar,Praveen, AU - Ramesh,V, AU - Sharma,Alpana, Y1 - 2019/10/16/ PY - 2019/07/11/received PY - 2019/09/19/revised PY - 2019/09/30/accepted PY - 2019/10/20/pubmed PY - 2019/10/20/medline PY - 2019/10/20/entrez KW - Hierarchical clustering KW - IL-17A/F KW - Inflammation KW - Th17 KW - Type 1 reactions SP - 154873 EP - 154873 JF - Cytokine JO - Cytokine VL - 126 N2 - Type 1 reactions (T1R) an inflammatory condition, of local skin patches in 30-40% leprosy patients during the course of MDT. IL-17A and IL-17F play an important role in regulating skin inflammation through neutrophils. In the present study, we have analyzed 18 of each T1R and Non-reactions (NR) patients through flow cytometry and qPCR. Interestingly we found that, CD3+CD4+ gated IL-17A+IL-17F+ cells were significantly high in T1R in both MLSA stimulated PBMCs and skin lesions as compared to NR leprosy patients. Hierarchical clustering analysis of gene expression showed that CXCL6, CXCL5, CCL20, CCL7, MMP13 and IL-17RB expression were significantly associated with IL-17A and IL-17F expression (Spearman r2 = 0.77 to 0.98), neutrophils and monocyte markers respectively. In this study, the inflammation noted in lesions of T1R is a different phenotype of Th17 which produce double positive IL-17A+IL17F+ and also contributes IL-17 producing neutrophils and thus would be useful for monitoring, diagnosis and treatment response before reactions episodes. SN - 1096-0023 UR - https://www.unboundmedicine.com/medline/citation/31629113/A_distinct_double_positive_IL-17A+/F+_T_helper_17_cells_induced_inflammation_leads_to_IL17_producing_neutrophils_in_Type_1_reaction_of_leprosy_patients L2 - https://linkinghub.elsevier.com/retrieve/pii/S1043-4666(19)30302-3 DB - PRIME DP - Unbound Medicine ER -