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Phytochemical identification from Lithocarpus polystachyus by UHPLC-Q-TOF-MS and its protein tyrosine phosphatase 1B and α-glucosidase activities.
Biomed Chromatogr 2019; :e4705BC

Abstract

Lithocarpus polystachyus leaves have become popular healthful tea due to its antidiabetic activity. In the present study, the phytochemical profiles were achieved by UHPLC-Q-TOF-MS in both positive and negative ion modes. A total of 17 compounds were tentatively characterized and identified by means of accurate mass and characteristic fragment ions. Total phenolic contents ranged from 9.0 to 13.4 g gallic acid equivalents (GAE)/100g of dry weight. In addition, α-glucosidase and protein tyrosine phosphatase 1B (PTP1B) inhibitory activities were evaluated. The L. polystachyus extracts significantly showed the α-glucosidase inhibition rate more than 88.1% at a concentration of 1.25 mg/mL. The acarbose was 93.6% at a concentration of 5 mg/mL. The PTP1B inhibition rate showed more than 84.3%. The antioxidant capacities were determined using DPPH, ABTS, and FRAP ranged from 50.5 to 72.5 g trolox, from 43.2 to 77.7 g trolox, and from 5.0 to 10.6 g BHT (equaling trolox or BHT per 100 g of dry weight), respectively. L. polystachyus could be considered as functional food for antidiabetic and antioxidative activities, due to its rich phenolic and dihydrochalcone contents.

Authors+Show Affiliations

Department of Pharmacy, Jining Medical University, Rizhao, Shandong, China.Department of Pharmacy, Jining Medical University, Rizhao, Shandong, China.Department of Pharmacy, Jining Medical University, Rizhao, Shandong, China.Department of Pharmacy, Jining Medical University, Rizhao, Shandong, China.Department of Pharmacy, Jining Medical University, Rizhao, Shandong, China.Department of Pharmacy, Jining Medical University, Rizhao, Shandong, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31629370

Citation

Meng, Ying, et al. "Phytochemical Identification From Lithocarpus Polystachyus By UHPLC-Q-TOF-MS and Its Protein Tyrosine Phosphatase 1B and Α-glucosidase Activities." Biomedical Chromatography : BMC, 2019, pp. e4705.
Meng Y, Ding L, Wang Y, et al. Phytochemical identification from Lithocarpus polystachyus by UHPLC-Q-TOF-MS and its protein tyrosine phosphatase 1B and α-glucosidase activities. Biomed Chromatogr. 2019.
Meng, Y., Ding, L., Wang, Y., Nie, Q. T., Xing, Y. Y., & Ren, Q. (2019). Phytochemical identification from Lithocarpus polystachyus by UHPLC-Q-TOF-MS and its protein tyrosine phosphatase 1B and α-glucosidase activities. Biomedical Chromatography : BMC, pp. e4705. doi:10.1002/bmc.4705.
Meng Y, et al. Phytochemical Identification From Lithocarpus Polystachyus By UHPLC-Q-TOF-MS and Its Protein Tyrosine Phosphatase 1B and Α-glucosidase Activities. Biomed Chromatogr. 2019 Oct 19;e4705. PubMed PMID: 31629370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytochemical identification from Lithocarpus polystachyus by UHPLC-Q-TOF-MS and its protein tyrosine phosphatase 1B and α-glucosidase activities. AU - Meng,Ying, AU - Ding,Lin, AU - Wang,Yuan, AU - Nie,Qi-Ting, AU - Xing,Yang-Yang, AU - Ren,Qiang, Y1 - 2019/10/19/ PY - 2019/04/28/received PY - 2019/09/08/revised PY - 2019/09/13/accepted PY - 2019/10/20/entrez PY - 2019/10/20/pubmed PY - 2019/10/20/medline KW - Lithocarpus polystachyus KW - PTP1B KW - UHPLC−Q-TOF-MS KW - α-glucosidase SP - e4705 EP - e4705 JF - Biomedical chromatography : BMC JO - Biomed. Chromatogr. N2 - Lithocarpus polystachyus leaves have become popular healthful tea due to its antidiabetic activity. In the present study, the phytochemical profiles were achieved by UHPLC-Q-TOF-MS in both positive and negative ion modes. A total of 17 compounds were tentatively characterized and identified by means of accurate mass and characteristic fragment ions. Total phenolic contents ranged from 9.0 to 13.4 g gallic acid equivalents (GAE)/100g of dry weight. In addition, α-glucosidase and protein tyrosine phosphatase 1B (PTP1B) inhibitory activities were evaluated. The L. polystachyus extracts significantly showed the α-glucosidase inhibition rate more than 88.1% at a concentration of 1.25 mg/mL. The acarbose was 93.6% at a concentration of 5 mg/mL. The PTP1B inhibition rate showed more than 84.3%. The antioxidant capacities were determined using DPPH, ABTS, and FRAP ranged from 50.5 to 72.5 g trolox, from 43.2 to 77.7 g trolox, and from 5.0 to 10.6 g BHT (equaling trolox or BHT per 100 g of dry weight), respectively. L. polystachyus could be considered as functional food for antidiabetic and antioxidative activities, due to its rich phenolic and dihydrochalcone contents. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/31629370/Phytochemical_identification_from_Lithocarpus_polystachyus_by_UHPLC-Q-TOF-MS_and_its_protein_tyrosine_phosphatase_1B_and_α-glucosidase_activities L2 - https://doi.org/10.1002/bmc.4705 DB - PRIME DP - Unbound Medicine ER -