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tRNA-derived fragments (tRFs) contribute to podocyte differentiation.

Abstract

Loss of glomerular podocytes is the crucial event in the progression of chronic kidney disease (CKD). tRNA-derived fragments (tRFs), a newfangled branch of small non-coding RNA (sncRNA), recently reported to play a vital part in several diseases. In present study, we aimed to detect and reveal the role of tRFs in podocyte differentiation. The expression levels of tRFs between undifferentiated and differentiated podocytes were sequenced by illumina nextseq 500, and further verified by quantitative RT-PCR. 69 upregulated and 70 downregulated tRFs in total were singled out (Fold change > 2, P < 0.05). Gene ontology (GO) analysis indicated they are involved in the biological processes of transcription, DNA-templated, positive regulation of transcription from RNA polymerase II promoter, angiogenesis, cell adhesion. Besides, KEGG analysis suggested that these differentially tRFs are associated with PI3K-Akt signaling pathway, Rap1 signaling pathway, Ras signaling pathway, MAPK signaling pathway, and Wnt signaling pathway. Therefore, the differentially tRFs might regulate the differentiation of podocyte and the process of CKD. The functions and mechanisms of tRFs in podocytes are needed to be further explored.

Authors+Show Affiliations

Department of Pediatric Nephrology, 2nd Affiliated Hospital of Nanjing Medical University, Nanjing, 210003, China.Department of Pediatric Nephrology, 2nd Affiliated Hospital of Nanjing Medical University, Nanjing, 210003, China; Department of Rheumatology, Children's Hospital of Soochow University, Suzhou, 215000, China.Department of Neonatology, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China.Department of Critical Care Medicine, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, China.Department of Pediatric Nephrology, 2nd Affiliated Hospital of Nanjing Medical University, Nanjing, 210003, China.Department of Pediatric Nephrology, 2nd Affiliated Hospital of Nanjing Medical University, Nanjing, 210003, China.Department of Pediatric Nephrology, 2nd Affiliated Hospital of Nanjing Medical University, Nanjing, 210003, China.Department of Pediatric Nephrology, 2nd Affiliated Hospital of Nanjing Medical University, Nanjing, 210003, China. Electronic address: weihuagan@njmu.edu.cn.Department of Pediatric Nephrology, 2nd Affiliated Hospital of Nanjing Medical University, Nanjing, 210003, China. Electronic address: njaiqing@njmu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31629473

Citation

Shi, Huimin, et al. "TRNA-derived Fragments (tRFs) Contribute to Podocyte Differentiation." Biochemical and Biophysical Research Communications, 2019.
Shi H, Yu M, Wu Y, et al. TRNA-derived fragments (tRFs) contribute to podocyte differentiation. Biochem Biophys Res Commun. 2019.
Shi, H., Yu, M., Wu, Y., Cao, Y., Li, S., Qu, G., ... Zhang, A. (2019). TRNA-derived fragments (tRFs) contribute to podocyte differentiation. Biochemical and Biophysical Research Communications, doi:10.1016/j.bbrc.2019.09.009.
Shi H, et al. TRNA-derived Fragments (tRFs) Contribute to Podocyte Differentiation. Biochem Biophys Res Commun. 2019 Oct 16; PubMed PMID: 31629473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - tRNA-derived fragments (tRFs) contribute to podocyte differentiation. AU - Shi,Huimin, AU - Yu,Minyi, AU - Wu,Yue, AU - Cao,Yuepeng, AU - Li,Shanwen, AU - Qu,Gaoting, AU - Gong,Jing, AU - Gan,Weihua, AU - Zhang,Aiqing, Y1 - 2019/10/16/ PY - 2019/07/09/received PY - 2019/09/01/revised PY - 2019/09/04/accepted PY - 2019/10/21/entrez PY - 2019/10/21/pubmed PY - 2019/10/21/medline KW - Chronic kidney disease KW - Differentiation KW - Glomerular KW - Podocyte loss KW - tRNA-derived fragments JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. N2 - Loss of glomerular podocytes is the crucial event in the progression of chronic kidney disease (CKD). tRNA-derived fragments (tRFs), a newfangled branch of small non-coding RNA (sncRNA), recently reported to play a vital part in several diseases. In present study, we aimed to detect and reveal the role of tRFs in podocyte differentiation. The expression levels of tRFs between undifferentiated and differentiated podocytes were sequenced by illumina nextseq 500, and further verified by quantitative RT-PCR. 69 upregulated and 70 downregulated tRFs in total were singled out (Fold change > 2, P < 0.05). Gene ontology (GO) analysis indicated they are involved in the biological processes of transcription, DNA-templated, positive regulation of transcription from RNA polymerase II promoter, angiogenesis, cell adhesion. Besides, KEGG analysis suggested that these differentially tRFs are associated with PI3K-Akt signaling pathway, Rap1 signaling pathway, Ras signaling pathway, MAPK signaling pathway, and Wnt signaling pathway. Therefore, the differentially tRFs might regulate the differentiation of podocyte and the process of CKD. The functions and mechanisms of tRFs in podocytes are needed to be further explored. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/31629473/tRNA-derived_fragments_(tRFs)_contribute_to_podocyte_differentiation L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(19)31718-8 DB - PRIME DP - Unbound Medicine ER -