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Effects of Brazilein on PSD-95 Protein Expression and Neurological Recovery in Mice after Sciatic Nerve Injury.
Neurosci Lett 2019; :134547NL

Abstract

PURPOSE

To evaluate the local nerve myelin recovery and the expression of PSD-95 protein and mRNA in the L4-L6 segment of the spinal cord after applying Brazilein to sciatic nerve injury BALB/c mice model and investigate the regulatory effects of Brazilein on myelin recovery after peripheral nerve injury.

METHODS

A total of 160 BALB/c mice were selected to establish the unilateral sciatic nerve injury model and randomly divided into four groups: saline blank control, Brazilein high-dose, medium-dose, and low-dose. Mice were assessed at different time points (1 w, 2 w, 4 w, 8 w) after sciatic nerve injury for the sciatic functional index (SFI) and sciatic nerve function recovery of the injured side by myelin Luxol Fast Blue (LFB) staining of the sciatic nerve. In addition, immunohistochemistry, real time-PCR, and Western blot were used to detect the PSD-95 expression in the spinal cord L4-L6 segments of the injured sciatic nerve at each time point.

RESULTS

The results of SFI and sciatic nerve function recovery, as well as, myelin LFB staining of the injured side indicated that all indexes of the Brazilein middle- and high-dose groups were significantly better than the low-dose and blank control groups at each time point. The PSD-95 expression in the L4-L6 segment of the spinal cord was statistically lower in the high- and medium-dose groups than in the low-dose and blank control groups at 1 w, 2 w, and 4 w, while the differences between the groups were not significant at 8 w.

CONCLUSION

Brazilein inhibits PSD-95 activation in the corresponding segment of sciatic nerve spinal cord in BALB/c mice after sciatic nerve injury, thereby inhibiting the excessive expression of free radicals and promoting myelin regeneration.

Authors+Show Affiliations

Department of Orthopedic, Affiliated Hospital of Chifeng University, Chifeng 024000, P R China. Electronic address: caojian2005088@hotmail.com.Department of Orthopedic, Affiliated Hospital of Chifeng University, Chifeng 024000, P R China. Electronic address: cflijinlong@163.com.Department of Ophthalmology, Affiliated Hospital of Chifeng University, Chifeng 024000, P R China. Electronic address: 34550926@qq.com.Department of Emergency,Affiliated Hospital of Chifeng University, Chifeng 024000, P R China. Electronic address: taobo_555@126.com.Department of Orthopedic, Affiliated Hospital of Chifeng University, Chifeng 024000, P R China. Electronic address: Liuzhongxing2002@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31629776

Citation

Jian, Ca, et al. "Effects of Brazilein On PSD-95 Protein Expression and Neurological Recovery in Mice After Sciatic Nerve Injury." Neuroscience Letters, 2019, p. 134547.
Jian C, Zhang L, Jinlong L, et al. Effects of Brazilein on PSD-95 Protein Expression and Neurological Recovery in Mice after Sciatic Nerve Injury. Neurosci Lett. 2019.
Jian, C., Zhang, L., Jinlong, L., Bo, T., & Liu, Z. (2019). Effects of Brazilein on PSD-95 Protein Expression and Neurological Recovery in Mice after Sciatic Nerve Injury. Neuroscience Letters, p. 134547. doi:10.1016/j.neulet.2019.134547.
Jian C, et al. Effects of Brazilein On PSD-95 Protein Expression and Neurological Recovery in Mice After Sciatic Nerve Injury. Neurosci Lett. 2019 Oct 17;134547. PubMed PMID: 31629776.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Brazilein on PSD-95 Protein Expression and Neurological Recovery in Mice after Sciatic Nerve Injury. AU - Jian,Ca, AU - Zhang,Limin, AU - Jinlong,Li, AU - Bo,Tao, AU - Liu,Zhongxing, Y1 - 2019/10/17/ PY - 2018/07/31/received PY - 2019/08/23/revised PY - 2019/10/09/accepted PY - 2019/10/21/entrez PY - 2019/10/21/pubmed PY - 2019/10/21/medline KW - Brazilein KW - PSD-95 KW - myelin recovery KW - sciatic nerve injury SP - 134547 EP - 134547 JF - Neuroscience letters JO - Neurosci. Lett. N2 - PURPOSE: To evaluate the local nerve myelin recovery and the expression of PSD-95 protein and mRNA in the L4-L6 segment of the spinal cord after applying Brazilein to sciatic nerve injury BALB/c mice model and investigate the regulatory effects of Brazilein on myelin recovery after peripheral nerve injury. METHODS: A total of 160 BALB/c mice were selected to establish the unilateral sciatic nerve injury model and randomly divided into four groups: saline blank control, Brazilein high-dose, medium-dose, and low-dose. Mice were assessed at different time points (1 w, 2 w, 4 w, 8 w) after sciatic nerve injury for the sciatic functional index (SFI) and sciatic nerve function recovery of the injured side by myelin Luxol Fast Blue (LFB) staining of the sciatic nerve. In addition, immunohistochemistry, real time-PCR, and Western blot were used to detect the PSD-95 expression in the spinal cord L4-L6 segments of the injured sciatic nerve at each time point. RESULTS: The results of SFI and sciatic nerve function recovery, as well as, myelin LFB staining of the injured side indicated that all indexes of the Brazilein middle- and high-dose groups were significantly better than the low-dose and blank control groups at each time point. The PSD-95 expression in the L4-L6 segment of the spinal cord was statistically lower in the high- and medium-dose groups than in the low-dose and blank control groups at 1 w, 2 w, and 4 w, while the differences between the groups were not significant at 8 w. CONCLUSION: Brazilein inhibits PSD-95 activation in the corresponding segment of sciatic nerve spinal cord in BALB/c mice after sciatic nerve injury, thereby inhibiting the excessive expression of free radicals and promoting myelin regeneration. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/31629776/Effects_of_Brazilein_on_PSD-95_Protein_Expression_and_Neurological_Recovery_in_Mice_after_Sciatic_Nerve_Injury L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(19)30650-0 DB - PRIME DP - Unbound Medicine ER -