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A label-free and carbon dots based fluorescent aptasensor for the detection of kanamycin in milk.

Abstract

A novel label-free aptasensor for kanamycin detection was constructed using gold nanoparticles (AuNPs) as absorber to quench the fluorescence of carbon dots (CDs) via the inner filter effect (IFE). The strategy was mainly relied on the fact that the absorption spectra of AuNPs overlapped with the fluorescence excitation spectra of fluorophores as well as the specific binding capacity of Ky2 aptamer to kanamycin. Upon adding kanamycin antibiotic, the free aptamer sequences are firstly exhausted to form some complexes, which leads to AuNPs aggregation in high salt concentration. Consequently, the absorber's absorption spectrum changes and no longer overlaps with the fluorescence emission spectrum of the CDs, which results in obvious fluorescence recovery of the aptasensor. Herein, the effects of some vital parameters like the type and number of nanoparticles on the fluorescent aptasensor have been investigated. Under optimal conditions, the proposed aptasensor can detect kanamycin in a linear range of 0.04-0.24 μM, with a limit of detection (LOD) as low as 18 nM. Moreover, the further studies also validate the applicability of the proposed aptasensor in milk samples, revealing that it may have enormous potential utility for practical kanamycin detection in food products in the future.

Authors+Show Affiliations

Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineering, Guizhou University, Guiyang, 550025, China.Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineering, Guizhou University, Guiyang, 550025, China.Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineering, Guizhou University, Guiyang, 550025, China.Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineering, Guizhou University, Guiyang, 550025, China.Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineering, Guizhou University, Guiyang, 550025, China; Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025, China. Electronic address: wuyg1357@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31629980

Citation

Wang, Jinlong, et al. "A Label-free and Carbon Dots Based Fluorescent Aptasensor for the Detection of Kanamycin in Milk." Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, vol. 226, 2019, p. 117651.
Wang J, Lu T, Hu Y, et al. A label-free and carbon dots based fluorescent aptasensor for the detection of kanamycin in milk. Spectrochim Acta A Mol Biomol Spectrosc. 2019;226:117651.
Wang, J., Lu, T., Hu, Y., Wang, X., & Wu, Y. (2019). A label-free and carbon dots based fluorescent aptasensor for the detection of kanamycin in milk. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 226, p. 117651. doi:10.1016/j.saa.2019.117651.
Wang J, et al. A Label-free and Carbon Dots Based Fluorescent Aptasensor for the Detection of Kanamycin in Milk. Spectrochim Acta A Mol Biomol Spectrosc. 2019 Oct 13;226:117651. PubMed PMID: 31629980.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A label-free and carbon dots based fluorescent aptasensor for the detection of kanamycin in milk. AU - Wang,Jinlong, AU - Lu,Tingting, AU - Hu,Yang, AU - Wang,Xueli, AU - Wu,Yuangen, Y1 - 2019/10/13/ PY - 2019/08/02/received PY - 2019/10/02/revised PY - 2019/10/09/accepted PY - 2019/10/21/pubmed PY - 2019/10/21/medline PY - 2019/10/21/entrez KW - Antibiotics KW - Aptasensor KW - Carbon dots KW - Fluorescent spectroscopy KW - Inner filter effect SP - 117651 EP - 117651 JF - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JO - Spectrochim Acta A Mol Biomol Spectrosc VL - 226 N2 - A novel label-free aptasensor for kanamycin detection was constructed using gold nanoparticles (AuNPs) as absorber to quench the fluorescence of carbon dots (CDs) via the inner filter effect (IFE). The strategy was mainly relied on the fact that the absorption spectra of AuNPs overlapped with the fluorescence excitation spectra of fluorophores as well as the specific binding capacity of Ky2 aptamer to kanamycin. Upon adding kanamycin antibiotic, the free aptamer sequences are firstly exhausted to form some complexes, which leads to AuNPs aggregation in high salt concentration. Consequently, the absorber's absorption spectrum changes and no longer overlaps with the fluorescence emission spectrum of the CDs, which results in obvious fluorescence recovery of the aptasensor. Herein, the effects of some vital parameters like the type and number of nanoparticles on the fluorescent aptasensor have been investigated. Under optimal conditions, the proposed aptasensor can detect kanamycin in a linear range of 0.04-0.24 μM, with a limit of detection (LOD) as low as 18 nM. Moreover, the further studies also validate the applicability of the proposed aptasensor in milk samples, revealing that it may have enormous potential utility for practical kanamycin detection in food products in the future. SN - 1873-3557 UR - https://www.unboundmedicine.com/medline/citation/31629980/A_label-free_and_carbon_dots_based_fluorescent_aptasensor_for_the_detection_of_kanamycin_in_milk L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-1425(19)31041-8 DB - PRIME DP - Unbound Medicine ER -