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Cytokine profile of CD4+CD25-FoxP3+ T cells in tumor-draining lymph nodes from patients with breast cancer.
Mol Immunol 2019; 116:90-97MI

Abstract

BACKGROUND

A T cell subtype with the CD4+CD25-FoxP3+ phenotype was recently described. We aimed to investigate the frequency of these cells and their ability to produce cytokines in tumor-draining lymph nodes from patients with breast cancer (BC).

MATERIALS AND METHODS

Mononuclear cells from lymph nodes of 20 patients with BC were activated and stained for appropriate markers. The cells were assayed with four-color flow cytometry.

RESULTS

A very small fraction of CD4+CD25-FoxP3+ cells produced cytokines at levels that were significantly lower than in the regulatory (CD4+CD25+FoxP3+) and effector cell (CD4+CD25+FoxP3-) subpopulations. The expression of IFNγ and IL-2 in the CD4+CD25-FoxP3+ subset was significantly higher than in Treg cells, but lower than in the effector subset. Conversely, IL-22 expression in Treg cells was significantly higher than in the CD4+CD25-FoxP3+ subpopulation. The expression of IL-10 in the CD4+CD25-FoxP3+ subset was also significantly higher than in effector cells.

CONCLUSION

We suggest that CD4+CD25-FoxP3+ cells in patients with BC are exhausted cells with an intermediate phenotype between effector and regulatory cells.

Authors+Show Affiliations

Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Breast Disease Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: ghaderia@sums.ac.ir.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31630080

Citation

Niakan, Andisheh, et al. "Cytokine Profile of CD4+CD25-FoxP3+ T Cells in Tumor-draining Lymph Nodes From Patients With Breast Cancer." Molecular Immunology, vol. 116, 2019, pp. 90-97.
Niakan A, Faghih Z, Talei AR, et al. Cytokine profile of CD4+CD25-FoxP3+ T cells in tumor-draining lymph nodes from patients with breast cancer. Mol Immunol. 2019;116:90-97.
Niakan, A., Faghih, Z., Talei, A. R., & Ghaderi, A. (2019). Cytokine profile of CD4+CD25-FoxP3+ T cells in tumor-draining lymph nodes from patients with breast cancer. Molecular Immunology, 116, pp. 90-97. doi:10.1016/j.molimm.2019.10.007.
Niakan A, et al. Cytokine Profile of CD4+CD25-FoxP3+ T Cells in Tumor-draining Lymph Nodes From Patients With Breast Cancer. Mol Immunol. 2019 Oct 17;116:90-97. PubMed PMID: 31630080.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytokine profile of CD4+CD25-FoxP3+ T cells in tumor-draining lymph nodes from patients with breast cancer. AU - Niakan,Andisheh, AU - Faghih,Zahra, AU - Talei,Abdol-Rasoul, AU - Ghaderi,Abbas, Y1 - 2019/10/17/ PY - 2019/07/07/received PY - 2019/10/07/revised PY - 2019/10/08/accepted PY - 2019/10/21/pubmed PY - 2019/10/21/medline PY - 2019/10/21/entrez KW - Breast cancer KW - CD4(+)CD25(−)FoxP3(+)cells KW - Treg KW - Tumor-draining lymph nodes SP - 90 EP - 97 JF - Molecular immunology JO - Mol. Immunol. VL - 116 N2 - BACKGROUND: A T cell subtype with the CD4+CD25-FoxP3+ phenotype was recently described. We aimed to investigate the frequency of these cells and their ability to produce cytokines in tumor-draining lymph nodes from patients with breast cancer (BC). MATERIALS AND METHODS: Mononuclear cells from lymph nodes of 20 patients with BC were activated and stained for appropriate markers. The cells were assayed with four-color flow cytometry. RESULTS: A very small fraction of CD4+CD25-FoxP3+ cells produced cytokines at levels that were significantly lower than in the regulatory (CD4+CD25+FoxP3+) and effector cell (CD4+CD25+FoxP3-) subpopulations. The expression of IFNγ and IL-2 in the CD4+CD25-FoxP3+ subset was significantly higher than in Treg cells, but lower than in the effector subset. Conversely, IL-22 expression in Treg cells was significantly higher than in the CD4+CD25-FoxP3+ subpopulation. The expression of IL-10 in the CD4+CD25-FoxP3+ subset was also significantly higher than in effector cells. CONCLUSION: We suggest that CD4+CD25-FoxP3+ cells in patients with BC are exhausted cells with an intermediate phenotype between effector and regulatory cells. SN - 1872-9142 UR - https://www.unboundmedicine.com/medline/citation/31630080/Cytokine_profile_of_CD4+CD25-FoxP3+_T_cells_in_tumor-draining_lymph_nodes_from_patients_with_breast_cancer L2 - https://linkinghub.elsevier.com/retrieve/pii/S0161-5890(19)30464-X DB - PRIME DP - Unbound Medicine ER -