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The effect of GnRH antagonist cetrorelix on Wnt signaling members in pubertal and adult mouse ovaries.

Abstract

Wide application of gonadotropin-releasing hormone (GnRH) agonists and antagonists for clinical purposes determines their effects on ovarian signaling pathways. Our study aimed to determine the localization, expression levels of Wnt signaling members in the pubertal and adult mouse ovary and the impact of GnRH antagonist cetrorelix on these signaling members. 0.5 mg/kg of cetrorelix was injected to 3-and 6-week-old mice for 2 weeks. At the end of injection, ovaries from 5 (5Ce)- to 8-week (8Ce)-old mice were embedded in paraffin for immunohistochemistry and homogenized for western blot to compare with control (5C-8C) and sham groups (5S-8S). WNT2 and WNT4 showed higher expression in thecal and stromal cells in adult mouse ovaries and only WNT4 expression was affected by cetrorelix. FZD1 was localized mainly in oocytes of pubertal ovaries and granulosa cells and oocytes of adult ovaries. FZD1 was reduced by cetrorelix in pubertal ovaries. FZD4 was abundantly localized in thecal and stromal cells of all groups and protein level was not affected by cetrorelix. LRP-6 was expressed mainly in oocytes and stromal cells of pubertal, oocytes of adult ovaries and its expression was reduced by cetrorelix in adult ovaries. CTNNB1 intensity in granulosa cells was the lowest in pubertal and the highest in adult ovaries and its expression was decreased by cetrorelix in adult ovaries. Cetrorelix affected the expression of specific members of the Wnt signaling depending on the developmental stage of mice, pointing out its possible interaction with gonadotropins during pubertal and adult stages.

Authors+Show Affiliations

Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Campus, 07070, Antalya, Turkey. Department of Histology and Embryology, Faculty of Medicine, Altinbas University, 34147, Istanbul, Turkey.Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Campus, 07070, Antalya, Turkey.Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Campus, 07070, Antalya, Turkey.Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Campus, 07070, Antalya, Turkey.Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Campus, 07070, Antalya, Turkey. gokhan_akkoyunlu@hotmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31630211

Citation

Tepekoy, Filiz, et al. "The Effect of GnRH Antagonist Cetrorelix On Wnt Signaling Members in Pubertal and Adult Mouse Ovaries." Histochemistry and Cell Biology, 2019.
Tepekoy F, Uysal F, Acar N, et al. The effect of GnRH antagonist cetrorelix on Wnt signaling members in pubertal and adult mouse ovaries. Histochem Cell Biol. 2019.
Tepekoy, F., Uysal, F., Acar, N., Ustunel, I., & Akkoyunlu, G. (2019). The effect of GnRH antagonist cetrorelix on Wnt signaling members in pubertal and adult mouse ovaries. Histochemistry and Cell Biology, doi:10.1007/s00418-019-01817-0.
Tepekoy F, et al. The Effect of GnRH Antagonist Cetrorelix On Wnt Signaling Members in Pubertal and Adult Mouse Ovaries. Histochem Cell Biol. 2019 Oct 19; PubMed PMID: 31630211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of GnRH antagonist cetrorelix on Wnt signaling members in pubertal and adult mouse ovaries. AU - Tepekoy,Filiz, AU - Uysal,Fatma, AU - Acar,Nuray, AU - Ustunel,Ismail, AU - Akkoyunlu,Gokhan, Y1 - 2019/10/19/ PY - 2019/09/10/accepted PY - 2019/10/21/entrez PY - 2019/10/21/pubmed PY - 2019/10/21/medline KW - CTNNB1 KW - FZD KW - LRP KW - Ovary KW - WNT JF - Histochemistry and cell biology JO - Histochem. Cell Biol. N2 - Wide application of gonadotropin-releasing hormone (GnRH) agonists and antagonists for clinical purposes determines their effects on ovarian signaling pathways. Our study aimed to determine the localization, expression levels of Wnt signaling members in the pubertal and adult mouse ovary and the impact of GnRH antagonist cetrorelix on these signaling members. 0.5 mg/kg of cetrorelix was injected to 3-and 6-week-old mice for 2 weeks. At the end of injection, ovaries from 5 (5Ce)- to 8-week (8Ce)-old mice were embedded in paraffin for immunohistochemistry and homogenized for western blot to compare with control (5C-8C) and sham groups (5S-8S). WNT2 and WNT4 showed higher expression in thecal and stromal cells in adult mouse ovaries and only WNT4 expression was affected by cetrorelix. FZD1 was localized mainly in oocytes of pubertal ovaries and granulosa cells and oocytes of adult ovaries. FZD1 was reduced by cetrorelix in pubertal ovaries. FZD4 was abundantly localized in thecal and stromal cells of all groups and protein level was not affected by cetrorelix. LRP-6 was expressed mainly in oocytes and stromal cells of pubertal, oocytes of adult ovaries and its expression was reduced by cetrorelix in adult ovaries. CTNNB1 intensity in granulosa cells was the lowest in pubertal and the highest in adult ovaries and its expression was decreased by cetrorelix in adult ovaries. Cetrorelix affected the expression of specific members of the Wnt signaling depending on the developmental stage of mice, pointing out its possible interaction with gonadotropins during pubertal and adult stages. SN - 1432-119X UR - https://www.unboundmedicine.com/medline/citation/31630211/The_effect_of_GnRH_antagonist_cetrorelix_on_Wnt_signaling_members_in_pubertal_and_adult_mouse_ovaries L2 - https://dx.doi.org/10.1007/s00418-019-01817-0 DB - PRIME DP - Unbound Medicine ER -