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Comparative Analysis of Number and Competence in Meiotic Maturation of Preovulatory Oocytes of C57Bl/6J Mice and Their F1 Hybrids after Stimulation with Gonadotropin.
Bull Exp Biol Med 2019; 167(5):702-705BE

Abstract

The study examined maturation of preovulatory germinal vesicles oocytes (GV oocytes) induced by gonadotropic hormone PMSG in the inbred C57Bl/6J mice (viewed as a gold standard for diverse biomedical studies) as well as in the first generation hybrid C57Bl/6J×СВА/lac and СВА/lac×C57Bl/6J mice at various ages. The most effective donors of GV oocytes were СВА/lac×C57Bl/6J mice (F1 hybrids) yielding 25±2 oocyte/mouse. In contrast, a significantly smaller number of GV oocytes can be isolated from the ovaries of female C57Bl/6J or C57Bl/6J×СВА/lac mice under the same conditions. At this, the greatest number of GV oocytes (42±4) can be retrieved from the ovaries of immature hybrid СВА/lac×C57Bl/6J mice aged 4 weeks. These mice demonstrated the largest share of GV oocytes, which attained MII stage during in vitro culturing. The data conclude that F1 hybrid СВА/lac×C57Bl/6J mice can be viewed as a handy experimental source yielding a large number of GV oocytes capable of meiotic maturation in a culture.

Authors+Show Affiliations

M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. zatsepina_olga@mail.ru.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31630300

Citation

Riabukha, Ya A., et al. "Comparative Analysis of Number and Competence in Meiotic Maturation of Preovulatory Oocytes of C57Bl/6J Mice and Their F1 Hybrids After Stimulation With Gonadotropin." Bulletin of Experimental Biology and Medicine, vol. 167, no. 5, 2019, pp. 702-705.
Riabukha YA, Petrova DA, Zatsepina OV. Comparative Analysis of Number and Competence in Meiotic Maturation of Preovulatory Oocytes of C57Bl/6J Mice and Their F1 Hybrids after Stimulation with Gonadotropin. Bull Exp Biol Med. 2019;167(5):702-705.
Riabukha, Y. A., Petrova, D. A., & Zatsepina, O. V. (2019). Comparative Analysis of Number and Competence in Meiotic Maturation of Preovulatory Oocytes of C57Bl/6J Mice and Their F1 Hybrids after Stimulation with Gonadotropin. Bulletin of Experimental Biology and Medicine, 167(5), pp. 702-705. doi:10.1007/s10517-019-04603-z.
Riabukha YA, Petrova DA, Zatsepina OV. Comparative Analysis of Number and Competence in Meiotic Maturation of Preovulatory Oocytes of C57Bl/6J Mice and Their F1 Hybrids After Stimulation With Gonadotropin. Bull Exp Biol Med. 2019;167(5):702-705. PubMed PMID: 31630300.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative Analysis of Number and Competence in Meiotic Maturation of Preovulatory Oocytes of C57Bl/6J Mice and Their F1 Hybrids after Stimulation with Gonadotropin. AU - Riabukha,Ya A, AU - Petrova,D A, AU - Zatsepina,O V, Y1 - 2019/10/19/ PY - 2018/07/31/received PY - 2019/10/21/pubmed PY - 2019/10/21/medline PY - 2019/10/21/entrez KW - C57Bl/6J, C57Bl/6J×СВА/lac, СВА/lac×C57Bl/6J mice KW - gonadotropin PMSG (eCG) KW - preovulatory (GV) oocytes SP - 702 EP - 705 JF - Bulletin of experimental biology and medicine JO - Bull. Exp. Biol. Med. VL - 167 IS - 5 N2 - The study examined maturation of preovulatory germinal vesicles oocytes (GV oocytes) induced by gonadotropic hormone PMSG in the inbred C57Bl/6J mice (viewed as a gold standard for diverse biomedical studies) as well as in the first generation hybrid C57Bl/6J×СВА/lac and СВА/lac×C57Bl/6J mice at various ages. The most effective donors of GV oocytes were СВА/lac×C57Bl/6J mice (F1 hybrids) yielding 25±2 oocyte/mouse. In contrast, a significantly smaller number of GV oocytes can be isolated from the ovaries of female C57Bl/6J or C57Bl/6J×СВА/lac mice under the same conditions. At this, the greatest number of GV oocytes (42±4) can be retrieved from the ovaries of immature hybrid СВА/lac×C57Bl/6J mice aged 4 weeks. These mice demonstrated the largest share of GV oocytes, which attained MII stage during in vitro culturing. The data conclude that F1 hybrid СВА/lac×C57Bl/6J mice can be viewed as a handy experimental source yielding a large number of GV oocytes capable of meiotic maturation in a culture. SN - 1573-8221 UR - https://www.unboundmedicine.com/medline/citation/31630300/Comparative_Analysis_of_Number_and_Competence_in_Meiotic_Maturation_of_Preovulatory_Oocytes_of_C57Bl/6J_Mice_and_Their_F1_Hybrids_after_Stimulation_with_Gonadotropin L2 - https://dx.doi.org/10.1007/s10517-019-04603-z DB - PRIME DP - Unbound Medicine ER -