Tags

Type your tag names separated by a space and hit enter

Gas Chromatography-Mass Spectrometry Analysis of Urinary Steroid Metabolomics for Detection of Early Signs of Adrenal Neoplasm Malignancy in Patients with Cushing's Syndrome.
Bull Exp Biol Med 2019; 167(5):676-680BE

Abstract

The metabolomics of urinary steroids was studied by gas chromatography-mass spectrometry in 25 patients with Cushing's syndrome without malignant potential and in 12 patients with malignant potential of adrenal neoplasms (Weiss score 1-3). Patients with adrenocortical adenoma (N=24) constituted the control group. In patients with Cushing's syndrome and malignant potential, increased urinary excretion of 16-oxo-androstendiol, tetrahydro-11-deoxycortisol, and 16-hydroxypregnendiol, which had 100% specificity and sensitivity >90% for the diagnosis of malignant potential. Additionally, non-classical 5-ene-pregnenes (16-OHpregnenolone, 21-OH-pregnenolone, 3β,16,20-pregnentriol, and 3β,17,20-pregnentriol) were identified. The revealed changes in the metabolomics of steroids can be early signs of malignant potential in patients with Cushing's syndrome. In patients with malignant potential, three signs of reduced activity of 11β-hydroxysteroid dehydrogenase type 2 were detected and in patients without malignant potential, one sign was found. In patients with and without malignant potential, three signs increased activity of 5β-reductase were found.

Authors+Show Affiliations

I. I. Mechnikov North-Western State Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia. velikanova46@gmail.com.I. I. Mechnikov North-Western State Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia.I. I. Mechnikov North-Western State Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia.I. I. Mechnikov North-Western State Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31630306

Citation

Velikanova, L I., et al. "Gas Chromatography-Mass Spectrometry Analysis of Urinary Steroid Metabolomics for Detection of Early Signs of Adrenal Neoplasm Malignancy in Patients With Cushing's Syndrome." Bulletin of Experimental Biology and Medicine, vol. 167, no. 5, 2019, pp. 676-680.
Velikanova LI, Shafigullina ZR, Vorokhobina NV, et al. Gas Chromatography-Mass Spectrometry Analysis of Urinary Steroid Metabolomics for Detection of Early Signs of Adrenal Neoplasm Malignancy in Patients with Cushing's Syndrome. Bull Exp Biol Med. 2019;167(5):676-680.
Velikanova, L. I., Shafigullina, Z. R., Vorokhobina, N. V., & Malevanaya, E. V. (2019). Gas Chromatography-Mass Spectrometry Analysis of Urinary Steroid Metabolomics for Detection of Early Signs of Adrenal Neoplasm Malignancy in Patients with Cushing's Syndrome. Bulletin of Experimental Biology and Medicine, 167(5), pp. 676-680. doi:10.1007/s10517-019-04597-8.
Velikanova LI, et al. Gas Chromatography-Mass Spectrometry Analysis of Urinary Steroid Metabolomics for Detection of Early Signs of Adrenal Neoplasm Malignancy in Patients With Cushing's Syndrome. Bull Exp Biol Med. 2019;167(5):676-680. PubMed PMID: 31630306.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gas Chromatography-Mass Spectrometry Analysis of Urinary Steroid Metabolomics for Detection of Early Signs of Adrenal Neoplasm Malignancy in Patients with Cushing's Syndrome. AU - Velikanova,L I, AU - Shafigullina,Z R, AU - Vorokhobina,N V, AU - Malevanaya,E V, Y1 - 2019/10/19/ PY - 2018/10/29/received PY - 2019/10/21/pubmed PY - 2019/10/21/medline PY - 2019/10/21/entrez KW - Cushing’s syndrome KW - gas chromatography-mass spectrometry KW - malignant potential KW - steroid profile in urine SP - 676 EP - 680 JF - Bulletin of experimental biology and medicine JO - Bull. Exp. Biol. Med. VL - 167 IS - 5 N2 - The metabolomics of urinary steroids was studied by gas chromatography-mass spectrometry in 25 patients with Cushing's syndrome without malignant potential and in 12 patients with malignant potential of adrenal neoplasms (Weiss score 1-3). Patients with adrenocortical adenoma (N=24) constituted the control group. In patients with Cushing's syndrome and malignant potential, increased urinary excretion of 16-oxo-androstendiol, tetrahydro-11-deoxycortisol, and 16-hydroxypregnendiol, which had 100% specificity and sensitivity >90% for the diagnosis of malignant potential. Additionally, non-classical 5-ene-pregnenes (16-OHpregnenolone, 21-OH-pregnenolone, 3β,16,20-pregnentriol, and 3β,17,20-pregnentriol) were identified. The revealed changes in the metabolomics of steroids can be early signs of malignant potential in patients with Cushing's syndrome. In patients with malignant potential, three signs of reduced activity of 11β-hydroxysteroid dehydrogenase type 2 were detected and in patients without malignant potential, one sign was found. In patients with and without malignant potential, three signs increased activity of 5β-reductase were found. SN - 1573-8221 UR - https://www.unboundmedicine.com/medline/citation/31630306/Gas_Chromatography-Mass_Spectrometry_Analysis_of_Urinary_Steroid_Metabolomics_for_Detection_of_Early_Signs_of_Adrenal_Neoplasm_Malignancy_in_Patients_with_Cushing's_Syndrome L2 - https://dx.doi.org/10.1007/s10517-019-04597-8 DB - PRIME DP - Unbound Medicine ER -