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Diphteria-tetanus-pertussis vaccine reduces specific IgE, inflammation and remodelling in an animal model of mite-induced respiratory allergy.
Vaccine 2019V

Abstract

BACKGROUND

Adjuvants, such as bacterial lipopolysaccharides, have been studied to improve the efficacy of allergen-specific immunotherapy. The Bordetella pertussis (Pw) vaccine has been shown to have a protective role in ovalbumin-induced asthma models. However, its role in allergy to mites is unknown. We evaluated the effects of the diphtheria-tetanus-pertussis (DTPw) vaccine on a murine model of respiratory allergy induced by Dermatophagoides pteronyssinus (Derp).

METHODS

In a 30-day protocol, BALB/c mice were immunized subcutaneously with saline or Derp, alone or in combination with diphtheria-tetanus (DT) or DTPw vaccines (days 0, 7 and 14). Subsequently, they underwent a daily intranasal challenge with saline or Derp (days 22-28) and were then sacrificed (day 29). We evaluated serum-specific immunoglobulins, bronchoalveolar lavage (BAL) cellularity, remodelling of the lower airways, density of polymorphonuclear leukocytes (PMNs) and acidic nasal mucus content.

RESULTS

The animals sensitized with Derp produced high levels of specific immunoglobulins, increased density of PMNs and nasal mucus content, and elevated BAL cellularity and remodelling. Vaccines led to a reduction in IgE levels, with the Derp-DTPw group being similar to the saline groups. The vaccinated groups had reductions of BAL cellularity and remodelling, with more expressive results in the Derp-DTPw group compared to the Derp-DT group. The DT and DTPw vaccines inhibited the nasal PMN infiltrate, and DTPw modulated the production of acidic nasal mucus.

CONCLUSIONS

The DTPw vaccine reduced serum specific IgE, nasal and pulmonary inflammation and remodelling of the lower airways.

Authors+Show Affiliations

Clinical Immunology and Allergy Division, University of Sao Paulo, Sao Paulo, Brazil; Laboratory of Experimental Therapeutics (LIM20), Department of Internal Medicine, University of Sao Paulo, Sao Paulo, Brazil. Electronic address: marcelovivoloaun@gmail.com.Laboratory of Experimental Therapeutics (LIM20), Department of Internal Medicine, University of Sao Paulo, Sao Paulo, Brazil.Laboratory of Experimental Therapeutics (LIM20), Department of Internal Medicine, University of Sao Paulo, Sao Paulo, Brazil.Laboratory of Experimental Therapeutics (LIM20), Department of Internal Medicine, University of Sao Paulo, Sao Paulo, Brazil.Clinical Immunology and Allergy Division, University of Sao Paulo, Sao Paulo, Brazil.Laboratory of Experimental Therapeutics (LIM20), Department of Internal Medicine, University of Sao Paulo, Sao Paulo, Brazil.Clinical Immunology and Allergy Division, University of Sao Paulo, Sao Paulo, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31630941

Citation

Aun, Marcelo Vivolo, et al. "Diphteria-tetanus-pertussis Vaccine Reduces Specific IgE, Inflammation and Remodelling in an Animal Model of Mite-induced Respiratory Allergy." Vaccine, 2019.
Aun MV, Almeida FM, Saraiva-Romanholo BM, et al. Diphteria-tetanus-pertussis vaccine reduces specific IgE, inflammation and remodelling in an animal model of mite-induced respiratory allergy. Vaccine. 2019.
Aun, M. V., Almeida, F. M., Saraiva-Romanholo, B. M., Martins, M. A., Kalil, J., Arantes-Costa, F. M., & Giavina-Bianchi, P. (2019). Diphteria-tetanus-pertussis vaccine reduces specific IgE, inflammation and remodelling in an animal model of mite-induced respiratory allergy. Vaccine, doi:10.1016/j.vaccine.2019.09.095.
Aun MV, et al. Diphteria-tetanus-pertussis Vaccine Reduces Specific IgE, Inflammation and Remodelling in an Animal Model of Mite-induced Respiratory Allergy. Vaccine. 2019 Oct 17; PubMed PMID: 31630941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diphteria-tetanus-pertussis vaccine reduces specific IgE, inflammation and remodelling in an animal model of mite-induced respiratory allergy. AU - Aun,Marcelo Vivolo, AU - Almeida,Francine Maria de, AU - Saraiva-Romanholo,Beatriz Mangueira, AU - Martins,Mílton de Arruda, AU - Kalil,Jorge, AU - Arantes-Costa,Fernanda Magalhães, AU - Giavina-Bianchi,Pedro, Y1 - 2019/10/17/ PY - 2018/10/02/received PY - 2019/08/20/revised PY - 2019/09/30/accepted PY - 2019/10/22/entrez KW - Animal model KW - Asthma KW - Bordetella pertussis KW - IgE KW - Remodelling KW - Vaccine JF - Vaccine JO - Vaccine N2 - BACKGROUND: Adjuvants, such as bacterial lipopolysaccharides, have been studied to improve the efficacy of allergen-specific immunotherapy. The Bordetella pertussis (Pw) vaccine has been shown to have a protective role in ovalbumin-induced asthma models. However, its role in allergy to mites is unknown. We evaluated the effects of the diphtheria-tetanus-pertussis (DTPw) vaccine on a murine model of respiratory allergy induced by Dermatophagoides pteronyssinus (Derp). METHODS: In a 30-day protocol, BALB/c mice were immunized subcutaneously with saline or Derp, alone or in combination with diphtheria-tetanus (DT) or DTPw vaccines (days 0, 7 and 14). Subsequently, they underwent a daily intranasal challenge with saline or Derp (days 22-28) and were then sacrificed (day 29). We evaluated serum-specific immunoglobulins, bronchoalveolar lavage (BAL) cellularity, remodelling of the lower airways, density of polymorphonuclear leukocytes (PMNs) and acidic nasal mucus content. RESULTS: The animals sensitized with Derp produced high levels of specific immunoglobulins, increased density of PMNs and nasal mucus content, and elevated BAL cellularity and remodelling. Vaccines led to a reduction in IgE levels, with the Derp-DTPw group being similar to the saline groups. The vaccinated groups had reductions of BAL cellularity and remodelling, with more expressive results in the Derp-DTPw group compared to the Derp-DT group. The DT and DTPw vaccines inhibited the nasal PMN infiltrate, and DTPw modulated the production of acidic nasal mucus. CONCLUSIONS: The DTPw vaccine reduced serum specific IgE, nasal and pulmonary inflammation and remodelling of the lower airways. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/31630941/Diphteria-tetanus-pertussis_vaccine_reduces_specific_IgE,_inflammation_and_remodelling_in_an_animal_model_of_mite-induced_respiratory_allergy L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(19)31338-6 DB - PRIME DP - Unbound Medicine ER -