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[Effects of Histone Methyltransferase Inhibitors on the Survival, Apoptosis and Cell Cycle of Raji Cells].
Sichuan Da Xue Xue Bao Yi Xue Ban 2019; 50(3):305-310SD

Abstract

Objective

To determine the effects of three histone methylase inhibitors UNC1999, DZNep and GSK343 on the survival, apoptosis and cell cycle of non-hodgkin's lymphoma Raji cells.

Methods

PCR amplified 16 and 18 exons of enhancer of zeste homolog 2 (EZH2) gene were detected. The expression of EZH2 in normal adult lymphocytes and Raji cells was detected by Western blot. The Raji cells were treated by UNC1999, DZNep and GSK343, followed by CCK-8 assays analyzing cell survival, flow cytometry detecting cell apoptosis and cell cycle, and Western blot detecting the expressions of EZH2 and H3K27 me3.

Results

The Sanger sequencing results showed that the Raji cells did not carry Y641 and A677 mutation sites of EZH2. The Western blot results showed high expressions of EZH2 in the Raji cells. The results of CCK-8 showed that UNC1999, DZNep and GSK343 inhibited cell survival, and the weakest effect was from DZNep. The flow cytometric assay showed that UNC1999, DZNep and GSK343 promoted apoptosis of the Raji cells, and the effect of UNC1999 was stronger than that of GSK343 and DZNep. The cell cycle was arrested at phase G 1/G 0 after treatment of the Raji cells with the three inhibitors, with UNC1999 triggering the most significant changes. The Western blot showed that UNC1999 and GSK343 inhibited the histone methylase activity of EZH2 and significantly reduced the expression of H3K27 me3.

Conclusion

EZH2 inhibitors can inhibit cell survival, promote cell apoptosis and arrest cell cycle at phase G 1/G 0 of Raji cells through reducing the expression of H3K27me3. UNC1999 has a stronger effect than GSK343 and DZNep.

Authors+Show Affiliations

Research Laboratory of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China.Research Laboratory of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China. Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China.Research Laboratory of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China. Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China.Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China.Research Laboratory of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China. Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China.Research Laboratory of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China. Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31631594

Citation

Xiao, Li-Rong, et al. "[Effects of Histone Methyltransferase Inhibitors On the Survival, Apoptosis and Cell Cycle of Raji Cells]." Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition, vol. 50, no. 3, 2019, pp. 305-310.
Xiao LR, Hou C, Zheng SJ, et al. [Effects of Histone Methyltransferase Inhibitors on the Survival, Apoptosis and Cell Cycle of Raji Cells]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2019;50(3):305-310.
Xiao, L. R., Hou, C., Zheng, S. J., Guo, B., Chen, D. N., & Yan, N. H. (2019). [Effects of Histone Methyltransferase Inhibitors on the Survival, Apoptosis and Cell Cycle of Raji Cells]. Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition, 50(3), pp. 305-310.
Xiao LR, et al. [Effects of Histone Methyltransferase Inhibitors On the Survival, Apoptosis and Cell Cycle of Raji Cells]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2019;50(3):305-310. PubMed PMID: 31631594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of Histone Methyltransferase Inhibitors on the Survival, Apoptosis and Cell Cycle of Raji Cells]. AU - Xiao,Li-Rong, AU - Hou,Chen, AU - Zheng,Shi-Jie, AU - Guo,Bo, AU - Chen,Da-Nian, AU - Yan,Nai-Hong, PY - 2019/10/22/entrez PY - 2019/10/22/pubmed PY - 2019/11/15/medline KW - Cell apoptosis KW - Cell cycle KW - EZH2 inhibitors KW - Raji lymphoma cells SP - 305 EP - 310 JF - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition JO - Sichuan Da Xue Xue Bao Yi Xue Ban VL - 50 IS - 3 N2 - Objective: To determine the effects of three histone methylase inhibitors UNC1999, DZNep and GSK343 on the survival, apoptosis and cell cycle of non-hodgkin's lymphoma Raji cells. Methods: PCR amplified 16 and 18 exons of enhancer of zeste homolog 2 (EZH2) gene were detected. The expression of EZH2 in normal adult lymphocytes and Raji cells was detected by Western blot. The Raji cells were treated by UNC1999, DZNep and GSK343, followed by CCK-8 assays analyzing cell survival, flow cytometry detecting cell apoptosis and cell cycle, and Western blot detecting the expressions of EZH2 and H3K27 me3. Results: The Sanger sequencing results showed that the Raji cells did not carry Y641 and A677 mutation sites of EZH2. The Western blot results showed high expressions of EZH2 in the Raji cells. The results of CCK-8 showed that UNC1999, DZNep and GSK343 inhibited cell survival, and the weakest effect was from DZNep. The flow cytometric assay showed that UNC1999, DZNep and GSK343 promoted apoptosis of the Raji cells, and the effect of UNC1999 was stronger than that of GSK343 and DZNep. The cell cycle was arrested at phase G 1/G 0 after treatment of the Raji cells with the three inhibitors, with UNC1999 triggering the most significant changes. The Western blot showed that UNC1999 and GSK343 inhibited the histone methylase activity of EZH2 and significantly reduced the expression of H3K27 me3. Conclusion: EZH2 inhibitors can inhibit cell survival, promote cell apoptosis and arrest cell cycle at phase G 1/G 0 of Raji cells through reducing the expression of H3K27me3. UNC1999 has a stronger effect than GSK343 and DZNep. SN - 1672-173X UR - https://www.unboundmedicine.com/medline/citation/31631594/[Effects_of_Histone_Methyltransferase_Inhibitors_on_the_Survival,_Apoptosis_and_Cell_Cycle_of_Raji_Cells] DB - PRIME DP - Unbound Medicine ER -