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Dissemination Of t437-SCCmecIV And Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus aureus Isolated From Skin Infections And Diabetic Foot Ulcers.
Infect Drug Resist 2019; 12:3197-3203ID

Abstract

Background

In a recent decade, the occurrence of S. aureus isolates with low-level oxacillin resistance, have been reported increasingly. The aim of this study was to estimate the prevalence of S. aureus with low-level of oxacillin resistance and to determine their molecular characteristics, including spa types, SCCmec types and presence of toxin genes.

Methods

A total of 249 S. aureus strains were analyzed. Antimicrobial susceptibility was preliminarily tested by the disk diffusion method, and further was verified with the E-test and agar dilution methods. All borderline oxacillin-resistant strains (BORSA) were screened for the mecA gene and virulence factors, including Panton-Valentine leukocidin (PVL). Staphylococcal cassette chromosome mec (SCCmec) typing and spa typing were also carried out.

Results

Twelve (4.8%) borderline oxacillin-resistant strains with MIC ≤4 µg/mL were identified. Almost all strains (11/12) were oxacillin-susceptible methicillin resistant S. aureus carrying mecA gene (OS-MRSA). Among the 12 bordeline strains, five spa types (t437, t037, t015, t216, t267) and two SCCmec types (III, IV) were identified, with the most prevalent being t437-SCCmecIV pvl-positive. The second most frequent spa type, t037-SCCmecIII, was sea-positive and did not produce coagulase. The majority of borderline strains originated from skin infections and diabetic foot ulcers and were multidrug-resistant (macrolides, lincosamides and chloramphenicol).

Conclusion

This study demonstrated that S. aureus with borderline resistance to oxacillin represented primarily SCCmecIV spa type t437 and coagulase-negative SCCmecIII spa type t037 and were isolated from skin infections and diabetic foot ulcers.

Authors+Show Affiliations

Department of Oral Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland.Department of Oral Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland.Department of Medical Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland.Laboratory of Clinical Microbiology, University Clinical Center, Gdansk, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31632105

Citation

Stańkowska, Maria, et al. "Dissemination of t437-SCCmecIV and Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus Aureus Isolated From Skin Infections and Diabetic Foot Ulcers." Infection and Drug Resistance, vol. 12, 2019, pp. 3197-3203.
Stańkowska M, Garbacz K, Piechowicz L, et al. Dissemination Of t437-SCCmecIV And Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus aureus Isolated From Skin Infections And Diabetic Foot Ulcers. Infect Drug Resist. 2019;12:3197-3203.
Stańkowska, M., Garbacz, K., Piechowicz, L., & Bronk, M. (2019). Dissemination Of t437-SCCmecIV And Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus aureus Isolated From Skin Infections And Diabetic Foot Ulcers. Infection and Drug Resistance, 12, pp. 3197-3203. doi:10.2147/IDR.S219557.
Stańkowska M, et al. Dissemination of t437-SCCmecIV and Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus Aureus Isolated From Skin Infections and Diabetic Foot Ulcers. Infect Drug Resist. 2019;12:3197-3203. PubMed PMID: 31632105.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dissemination Of t437-SCCmecIV And Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus aureus Isolated From Skin Infections And Diabetic Foot Ulcers. AU - Stańkowska,Maria, AU - Garbacz,Katarzyna, AU - Piechowicz,Lidia, AU - Bronk,Marek, Y1 - 2019/10/10/ PY - 2019/06/14/received PY - 2019/09/07/accepted PY - 2019/10/22/entrez PY - 2019/10/22/pubmed PY - 2019/10/22/medline KW - MRSA KW - OS-MRSA KW - Staphylococcus aureus KW - borderline oxacillin-resistant S. aureus KW - low-level oxacillin resistance SP - 3197 EP - 3203 JF - Infection and drug resistance JO - Infect Drug Resist VL - 12 N2 - Background: In a recent decade, the occurrence of S. aureus isolates with low-level oxacillin resistance, have been reported increasingly. The aim of this study was to estimate the prevalence of S. aureus with low-level of oxacillin resistance and to determine their molecular characteristics, including spa types, SCCmec types and presence of toxin genes. Methods: A total of 249 S. aureus strains were analyzed. Antimicrobial susceptibility was preliminarily tested by the disk diffusion method, and further was verified with the E-test and agar dilution methods. All borderline oxacillin-resistant strains (BORSA) were screened for the mecA gene and virulence factors, including Panton-Valentine leukocidin (PVL). Staphylococcal cassette chromosome mec (SCCmec) typing and spa typing were also carried out. Results: Twelve (4.8%) borderline oxacillin-resistant strains with MIC ≤4 µg/mL were identified. Almost all strains (11/12) were oxacillin-susceptible methicillin resistant S. aureus carrying mecA gene (OS-MRSA). Among the 12 bordeline strains, five spa types (t437, t037, t015, t216, t267) and two SCCmec types (III, IV) were identified, with the most prevalent being t437-SCCmecIV pvl-positive. The second most frequent spa type, t037-SCCmecIII, was sea-positive and did not produce coagulase. The majority of borderline strains originated from skin infections and diabetic foot ulcers and were multidrug-resistant (macrolides, lincosamides and chloramphenicol). Conclusion: This study demonstrated that S. aureus with borderline resistance to oxacillin represented primarily SCCmecIV spa type t437 and coagulase-negative SCCmecIII spa type t037 and were isolated from skin infections and diabetic foot ulcers. SN - 1178-6973 UR - https://www.unboundmedicine.com/medline/citation/31632105/Dissemination_Of_t437-SCCmecIV_And_Coagulase-Negative_t037-SCCmecIII_Types_Among_Borderline_Oxacillin-Resistant_Staphylococcus_aureus_Isolated_From_Skin_Infections_And_Diabetic_Foot_Ulcers L2 - https://dx.doi.org/10.2147/IDR.S219557 DB - PRIME DP - Unbound Medicine ER -