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Conversion of xanthine dehydrogenase to oxidase in ischemic rat intestine: a reevaluation.
Am J Physiol. 1988 May; 254(5 Pt 1):G768-74.AJ

Abstract

Oxygen radicals derived from xanthine oxidase (XO) are important mediators of the cellular injury associated with reperfusion of ischemic intestine, stomach, liver, kidney, and pancreas. XO exists in nonischemic tissue predominantly as xanthine dehydrogenase (XDH) and converts to oxygen radical-producing XO with ischemia. Grinding intestine under liquid nitrogen and placing the powder in phosphate buffer (pH 7.0) containing thiol reductants and protease inhibitors adequately preserved total XDH + XO activity and the percentage in the oxidase form (%XO) for 24 h. Total activity in nonischemic intestine ranged from 374 nmol.min-1.g-1 in duodenum to 138 nmol.min-1.g-1 in ileum, while XO activity was approximately 19% of total activity throughout the entire small intestine. The rate of XDH conversion to XO during normothermic ischemia varied only slightly throughout the intestine, increasing 13% per hour to 34, 46, and 61% XO after 1, 2, and 3 h of ischemia, respectively. Our results contrast with previous reports where XDH conversion to XO occurred within 60 s ischemia but are consistent with physiological and morphological evidence of ischemic injury and provide further support for involvement of XO in intestinal injury associated with ischemia.

Authors+Show Affiliations

Department of Anesthesiology, University of Alabama, Birmingham 35233.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3163236

Citation

Parks, D A., et al. "Conversion of Xanthine Dehydrogenase to Oxidase in Ischemic Rat Intestine: a Reevaluation." The American Journal of Physiology, vol. 254, no. 5 Pt 1, 1988, pp. G768-74.
Parks DA, Williams TK, Beckman JS. Conversion of xanthine dehydrogenase to oxidase in ischemic rat intestine: a reevaluation. Am J Physiol. 1988;254(5 Pt 1):G768-74.
Parks, D. A., Williams, T. K., & Beckman, J. S. (1988). Conversion of xanthine dehydrogenase to oxidase in ischemic rat intestine: a reevaluation. The American Journal of Physiology, 254(5 Pt 1), G768-74.
Parks DA, Williams TK, Beckman JS. Conversion of Xanthine Dehydrogenase to Oxidase in Ischemic Rat Intestine: a Reevaluation. Am J Physiol. 1988;254(5 Pt 1):G768-74. PubMed PMID: 3163236.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Conversion of xanthine dehydrogenase to oxidase in ischemic rat intestine: a reevaluation. AU - Parks,D A, AU - Williams,T K, AU - Beckman,J S, PY - 1988/5/1/pubmed PY - 1988/5/1/medline PY - 1988/5/1/entrez SP - G768 EP - 74 JF - The American journal of physiology JO - Am J Physiol VL - 254 IS - 5 Pt 1 N2 - Oxygen radicals derived from xanthine oxidase (XO) are important mediators of the cellular injury associated with reperfusion of ischemic intestine, stomach, liver, kidney, and pancreas. XO exists in nonischemic tissue predominantly as xanthine dehydrogenase (XDH) and converts to oxygen radical-producing XO with ischemia. Grinding intestine under liquid nitrogen and placing the powder in phosphate buffer (pH 7.0) containing thiol reductants and protease inhibitors adequately preserved total XDH + XO activity and the percentage in the oxidase form (%XO) for 24 h. Total activity in nonischemic intestine ranged from 374 nmol.min-1.g-1 in duodenum to 138 nmol.min-1.g-1 in ileum, while XO activity was approximately 19% of total activity throughout the entire small intestine. The rate of XDH conversion to XO during normothermic ischemia varied only slightly throughout the intestine, increasing 13% per hour to 34, 46, and 61% XO after 1, 2, and 3 h of ischemia, respectively. Our results contrast with previous reports where XDH conversion to XO occurred within 60 s ischemia but are consistent with physiological and morphological evidence of ischemic injury and provide further support for involvement of XO in intestinal injury associated with ischemia. SN - 0002-9513 UR - https://www.unboundmedicine.com/medline/citation/3163236/Conversion_of_xanthine_dehydrogenase_to_oxidase_in_ischemic_rat_intestine:_a_reevaluation_ L2 - https://journals.physiology.org/doi/10.1152/ajpgi.1988.254.5.G768?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -