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Expression of VEGFA-regulating miRNAs and mortality in wet AMD.
J Cell Mol Med 2019; 23(12):8464-8471JC

Abstract

MicroRNAs (miRNAs) regulate gene expression; many of them act in the retinal pigment epithelium (RPE), and RPE degeneration is known to be a critical factor in age-related macular degeneration (AMD). Repeated injections with anti-VEGFA (vascular endothelial growth factor A) are the only effective therapy in wet AMD. We investigated the correlation between the expression of 18 miRNAs involved in the regulation of the VEGFA gene in serum of 76 wet AMD patients and 70 controls. Efficacy of anti-VEGFA treatment was evaluated by counting the number of injections delivered up to 12 years. In addition, we compared the relative numbers of deaths in patient with AMD and control groups. We observed a decreased expression of miR-34-5p, miR-126-3p, miR-145-5p and miR-205-5p in wet AMD patients as compared with controls. These miRNAs are involved in the regulation of angiogenesis, cytoprotection and protein clearance. No miRNA was significantly correlated with the treatment outcome. Wet AMD patients had greater mortality than controls, and their survival was inversely associated with the number of anti-VEGFA injections per year. No association was observed between miRNA expression and mortality. Our study emphasizes the need to clarify the role of miRNA regulation in AMD pathogenesis.

Authors+Show Affiliations

Department of Molecular Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.Department of Haemostatic Disorders, Medical University, Lodz, Poland.Helsinki Retina Research Group, University of Helsinki, Helsinki, Finland. Department of Ophthalmology, Kymenlaakso Central Hospital, Kotka, Finland.Department of Ophthalmology, University of Eastern Finland, Kuopio, Finland.Department of Ophthalmology, University of Eastern Finland, Kuopio, Finland.Department of Ophthalmology, Tampere University Hospital, Tampere, Finland.Department of Ophthalmology, Tampere University Hospital, Tampere, Finland.Department of Vitreoretinal Surgery, Medical University of Lublin, Lublin, Poland.Department of Vitreoretinal Surgery, Medical University of Lublin, Lublin, Poland.Department of Cancer Genetics, Medical University of Lublin, Lublin, Poland.Department of Cancer Genetics, Medical University of Lublin, Lublin, Poland.Department of Ophthalmology, University of Eastern Finland, Kuopio, Finland. Department of Ophthalmology, Kuopio University Hospital, Kuopio, Finland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31633290

Citation

Blasiak, Janusz, et al. "Expression of VEGFA-regulating miRNAs and Mortality in Wet AMD." Journal of Cellular and Molecular Medicine, vol. 23, no. 12, 2019, pp. 8464-8471.
Blasiak J, Watala C, Tuuminen R, et al. Expression of VEGFA-regulating miRNAs and mortality in wet AMD. J Cell Mol Med. 2019;23(12):8464-8471.
Blasiak, J., Watala, C., Tuuminen, R., Kivinen, N., Koskela, A., Uusitalo-Järvinen, H., ... Kaarniranta, K. (2019). Expression of VEGFA-regulating miRNAs and mortality in wet AMD. Journal of Cellular and Molecular Medicine, 23(12), pp. 8464-8471. doi:10.1111/jcmm.14731.
Blasiak J, et al. Expression of VEGFA-regulating miRNAs and Mortality in Wet AMD. J Cell Mol Med. 2019;23(12):8464-8471. PubMed PMID: 31633290.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of VEGFA-regulating miRNAs and mortality in wet AMD. AU - Blasiak,Janusz, AU - Watala,Cezary, AU - Tuuminen,Raimo, AU - Kivinen,Niko, AU - Koskela,Ali, AU - Uusitalo-Järvinen,Hannele, AU - Tuulonen,Anja, AU - Winiarczyk,Mateusz, AU - Mackiewicz,Jerzy, AU - Zmorzyński,Szymon, AU - Filip,Agata, AU - Kaarniranta,Kai, Y1 - 2019/10/21/ PY - 2019/04/12/received PY - 2019/09/12/revised PY - 2019/09/23/accepted PY - 2019/12/01/pmc-release PY - 2019/10/22/pubmed PY - 2019/10/22/medline PY - 2019/10/22/entrez KW - AMD KW - age-related macular degeneration KW - anti-VEGFA injection KW - miRNA KW - mortality KW - wet AMD therapy SP - 8464 EP - 8471 JF - Journal of cellular and molecular medicine JO - J. Cell. Mol. Med. VL - 23 IS - 12 N2 - MicroRNAs (miRNAs) regulate gene expression; many of them act in the retinal pigment epithelium (RPE), and RPE degeneration is known to be a critical factor in age-related macular degeneration (AMD). Repeated injections with anti-VEGFA (vascular endothelial growth factor A) are the only effective therapy in wet AMD. We investigated the correlation between the expression of 18 miRNAs involved in the regulation of the VEGFA gene in serum of 76 wet AMD patients and 70 controls. Efficacy of anti-VEGFA treatment was evaluated by counting the number of injections delivered up to 12 years. In addition, we compared the relative numbers of deaths in patient with AMD and control groups. We observed a decreased expression of miR-34-5p, miR-126-3p, miR-145-5p and miR-205-5p in wet AMD patients as compared with controls. These miRNAs are involved in the regulation of angiogenesis, cytoprotection and protein clearance. No miRNA was significantly correlated with the treatment outcome. Wet AMD patients had greater mortality than controls, and their survival was inversely associated with the number of anti-VEGFA injections per year. No association was observed between miRNA expression and mortality. Our study emphasizes the need to clarify the role of miRNA regulation in AMD pathogenesis. SN - 1582-4934 UR - https://www.unboundmedicine.com/medline/citation/31633290/Expression_of_VEGFA-regulating_miRNAs_and_mortality_in_wet_AMD L2 - https://doi.org/10.1111/jcmm.14731 DB - PRIME DP - Unbound Medicine ER -