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MiR-205 promotes endothelial progenitor cell angiogenesis and deep vein thrombosis recanalization and resolution by targeting PTEN to regulate Akt/autophagy pathway and MMP2 expression.
J Cell Mol Med 2019; 23(12):8493-8504JC

Abstract

MicroRNAs (MiRNAs, MiRs) represent a class of conserved small non-coding RNAs that affect post-transcriptional gene regulation and play a vital role in angiogenesis, proliferation, apoptosis, migration and invasion. They are essential for a wide range of physiological and pathological processes, especially for vascular diseases. However, data concerning miRNAs in endothelial progenitor cells (EPCs) and deep vein thrombosis (DVT) remain incomplete. We explored miRNAs that modulate angiogenesis in EPCs and thrombolysis, and analysed their underlying mechanisms using a DVT model, dual-luciferase reporter assay, qRT-PCR, Western blot, immunofluorescence staining, flow cytometry analysis, CCK-8 assay, angiogenesis assay, wound healing and Transwell assay. We found that miR-205 enhanced the homing ability of EPCs to DVT sites and promoted thrombosis resolution and recanalization, which significantly reduced venous thrombus. Additionally, we demonstrated that miR-205 overexpression significantly enhanced angiogenesis in vivo and in vitro, migration, invasion, F-actin filaments and proliferation in EPCs, and inhibited cell apoptosis. Conversely, down-regulation of miR-205 played the opposite role in EPCs. Importantly, this study demonstrated that miR-205 directly targeted PTEN to modulate the Akt/autophagy pathway and MMP2 expression, subsequently playing a key role in EPC function and DVT recanalization and resolution. These results elucidated the pro-angiogenesis effects of miR-205 in EPCs and established it as a potential target for DVT treatment.

Authors+Show Affiliations

Department of Vascular Surgery, The Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing, China. Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China.Department of Vascular Surgery, The Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.Department of Vascular Surgery, The Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China.Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China.Department of Vascular Surgery, Fengyang County People's Hospital, Chuzhou, China.Department of Vascular Surgery, The Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.Department of Vascular Surgery, The Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31633295

Citation

Sun, Li-Li, et al. "MiR-205 Promotes Endothelial Progenitor Cell Angiogenesis and Deep Vein Thrombosis Recanalization and Resolution By Targeting PTEN to Regulate Akt/autophagy Pathway and MMP2 Expression." Journal of Cellular and Molecular Medicine, vol. 23, no. 12, 2019, pp. 8493-8504.
Sun LL, Xiao L, Du XL, et al. MiR-205 promotes endothelial progenitor cell angiogenesis and deep vein thrombosis recanalization and resolution by targeting PTEN to regulate Akt/autophagy pathway and MMP2 expression. J Cell Mol Med. 2019;23(12):8493-8504.
Sun, L. L., Xiao, L., Du, X. L., Hong, L., Li, C. L., Jiao, J., ... Li, X. Q. (2019). MiR-205 promotes endothelial progenitor cell angiogenesis and deep vein thrombosis recanalization and resolution by targeting PTEN to regulate Akt/autophagy pathway and MMP2 expression. Journal of Cellular and Molecular Medicine, 23(12), pp. 8493-8504. doi:10.1111/jcmm.14739.
Sun LL, et al. MiR-205 Promotes Endothelial Progenitor Cell Angiogenesis and Deep Vein Thrombosis Recanalization and Resolution By Targeting PTEN to Regulate Akt/autophagy Pathway and MMP2 Expression. J Cell Mol Med. 2019;23(12):8493-8504. PubMed PMID: 31633295.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MiR-205 promotes endothelial progenitor cell angiogenesis and deep vein thrombosis recanalization and resolution by targeting PTEN to regulate Akt/autophagy pathway and MMP2 expression. AU - Sun,Li-Li, AU - Xiao,Lun, AU - Du,Xiao-Long, AU - Hong,Lei, AU - Li,Cheng-Long, AU - Jiao,Jian, AU - Li,Wen-Dong, AU - Li,Xiao-Qiang, Y1 - 2019/10/21/ PY - 2019/07/03/received PY - 2019/08/21/revised PY - 2019/09/13/accepted PY - 2019/12/01/pmc-release PY - 2019/10/22/pubmed PY - 2019/10/22/medline PY - 2019/10/22/entrez KW - angiogenesis KW - deep vein thrombosis KW - endothelial progenitor cell KW - microRNA KW - migration SP - 8493 EP - 8504 JF - Journal of cellular and molecular medicine JO - J. Cell. Mol. Med. VL - 23 IS - 12 N2 - MicroRNAs (MiRNAs, MiRs) represent a class of conserved small non-coding RNAs that affect post-transcriptional gene regulation and play a vital role in angiogenesis, proliferation, apoptosis, migration and invasion. They are essential for a wide range of physiological and pathological processes, especially for vascular diseases. However, data concerning miRNAs in endothelial progenitor cells (EPCs) and deep vein thrombosis (DVT) remain incomplete. We explored miRNAs that modulate angiogenesis in EPCs and thrombolysis, and analysed their underlying mechanisms using a DVT model, dual-luciferase reporter assay, qRT-PCR, Western blot, immunofluorescence staining, flow cytometry analysis, CCK-8 assay, angiogenesis assay, wound healing and Transwell assay. We found that miR-205 enhanced the homing ability of EPCs to DVT sites and promoted thrombosis resolution and recanalization, which significantly reduced venous thrombus. Additionally, we demonstrated that miR-205 overexpression significantly enhanced angiogenesis in vivo and in vitro, migration, invasion, F-actin filaments and proliferation in EPCs, and inhibited cell apoptosis. Conversely, down-regulation of miR-205 played the opposite role in EPCs. Importantly, this study demonstrated that miR-205 directly targeted PTEN to modulate the Akt/autophagy pathway and MMP2 expression, subsequently playing a key role in EPC function and DVT recanalization and resolution. These results elucidated the pro-angiogenesis effects of miR-205 in EPCs and established it as a potential target for DVT treatment. SN - 1582-4934 UR - https://www.unboundmedicine.com/medline/citation/31633295/MiR-205_promotes_endothelial_progenitor_cell_angiogenesis_and_deep_vein_thrombosis_recanalization_and_resolution_by_targeting_PTEN_to_regulate_Akt/autophagy_pathway_and_MMP2_expression L2 - https://doi.org/10.1111/jcmm.14739 DB - PRIME DP - Unbound Medicine ER -