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The unique antimicrobial peptide repertoire of stick insects.
Dev Comp Immunol 2020; 103:103471DC

Abstract

The comparative analysis of innate immunity across different insect taxa has revealed unanticipated evolutionary plasticity, providing intriguing examples of immunity-related effector gene expansion and loss. Phasmatodea, the stick and leaf insects, is an order of hemimetabolous insects that can provide insight into ancestral innate immunity genes lost by later insect clades. We injected the stick insect Peruphasma schultei with a mixture of microbial elicitors to activate a strong immune response, followed by RNA-Seq analysis to screen for induced immunity-related effector genes. This revealed a highly diverse spectrum of antimicrobial peptides (AMPs) belonging to the attacin, coleoptericin, defensin, thaumatin, and tachystatin families. In addition, we identified a large group of short, cysteine-rich putative AMPs, some of which were strongly elicited. The immunity-related effector gene repertoire also included c-type and i-type lysozymes and several pattern-recognition proteins, such as proteins that recognize Gram-negative bacteria and peptidoglycans. Finally, we identified 45 hemolymph lipopolysaccharide-binding protein sequences, an unusually large number for insects. Taken together, our results indicate that at least some phasmids synthesize a broad spectrum of diverse AMPs that deserve further in-depth analysis.

Authors+Show Affiliations

Department of Entomology, National Taiwan University, Taipei, Taiwan. Electronic address: mshelomi@ntu.edu.tw.Max Planck Institute for Chemical Ecology, Jena, Germany.Institute for Insect Biotechnology, Justus Liebig University of Giessen, Giessen, Germany.Max Planck Institute for Chemical Ecology, Jena, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31634521

Citation

Shelomi, Matan, et al. "The Unique Antimicrobial Peptide Repertoire of Stick Insects." Developmental and Comparative Immunology, vol. 103, 2020, p. 103471.
Shelomi M, Jacobs C, Vilcinskas A, et al. The unique antimicrobial peptide repertoire of stick insects. Dev Comp Immunol. 2020;103:103471.
Shelomi, M., Jacobs, C., Vilcinskas, A., & Vogel, H. (2020). The unique antimicrobial peptide repertoire of stick insects. Developmental and Comparative Immunology, 103, p. 103471. doi:10.1016/j.dci.2019.103471.
Shelomi M, et al. The Unique Antimicrobial Peptide Repertoire of Stick Insects. Dev Comp Immunol. 2020;103:103471. PubMed PMID: 31634521.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The unique antimicrobial peptide repertoire of stick insects. AU - Shelomi,Matan, AU - Jacobs,Chris, AU - Vilcinskas,Andreas, AU - Vogel,Heiko, Y1 - 2019/10/18/ PY - 2019/07/24/received PY - 2019/08/17/revised PY - 2019/08/18/accepted PY - 2019/10/22/pubmed PY - 2019/10/22/medline PY - 2019/10/22/entrez SP - 103471 EP - 103471 JF - Developmental and comparative immunology JO - Dev. Comp. Immunol. VL - 103 N2 - The comparative analysis of innate immunity across different insect taxa has revealed unanticipated evolutionary plasticity, providing intriguing examples of immunity-related effector gene expansion and loss. Phasmatodea, the stick and leaf insects, is an order of hemimetabolous insects that can provide insight into ancestral innate immunity genes lost by later insect clades. We injected the stick insect Peruphasma schultei with a mixture of microbial elicitors to activate a strong immune response, followed by RNA-Seq analysis to screen for induced immunity-related effector genes. This revealed a highly diverse spectrum of antimicrobial peptides (AMPs) belonging to the attacin, coleoptericin, defensin, thaumatin, and tachystatin families. In addition, we identified a large group of short, cysteine-rich putative AMPs, some of which were strongly elicited. The immunity-related effector gene repertoire also included c-type and i-type lysozymes and several pattern-recognition proteins, such as proteins that recognize Gram-negative bacteria and peptidoglycans. Finally, we identified 45 hemolymph lipopolysaccharide-binding protein sequences, an unusually large number for insects. Taken together, our results indicate that at least some phasmids synthesize a broad spectrum of diverse AMPs that deserve further in-depth analysis. SN - 1879-0089 UR - https://www.unboundmedicine.com/medline/citation/31634521/The_unique_antimicrobial_peptide_repertoire_of_stick_insects L2 - https://linkinghub.elsevier.com/retrieve/pii/S0145-305X(19)30347-7 DB - PRIME DP - Unbound Medicine ER -