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A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations.
Fungal Genet Biol 2019; 134:103284FG

Abstract

The ability of Saccharomyces cerevisiae to reconstitute viability after strong peroxide-induced oxidative stress during liquid holding (LH) in non-nutrient medium has been compared with that of Ustilago maydis. It was found that like U. maydis, S. cerevisiae was capable of reconstituting viability through multiplication of the survivors. However, differences were observed in the pattern of their response: (i) the reconstitution of viability was slower in S. cerevisiae; (ii) before the viability was progressively increasing the treated samples of this fungus reproducibly passed through a phase of additional decrease of the surviving fraction and (iii) the final yields of viable cells attained in S. cerevisiae were below those achieved by U. maydis. The reason for the relative superiority of U. maydis is twofold: (1) early initiated and faster degradation and leakage of the intracellular biomolecules and (2) greater ability of U. maydis cells to recycle damaged and released intracellular compounds. Conceptually similar studies extended to another oxidative-stress-inducing condition, namely desiccation, indicated that the marked differences between these fungi in their patterns of the post-stress regrowth, cellular leakage and macromolecule decomposition are reproduced during LH of desiccated cells. The concordance of the findings obtained upon these two approaches was also corroborated by an analysis of the post-desiccation LH response of U. maydis mutants (adr1, did4, kel1 and tbp1) that were previously identified as defective in post-peroxide LH restitution of viability. We discuss the findings in terms of their possible relevance to the mechanisms of the ecological and evolutionary adaptation of free-living microorganisms to fluctuating and severely inhospitable environments.

Authors+Show Affiliations

Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Serbia.Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Serbia. Electronic address: mdkojic@imgge.bg.ac.rs.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31634563

Citation

Milisavljevic, Mira, and Milorad Kojic. "A Comparative Study of Liquid Holding Restitution of Viability After Oxidative Stress in Ustilago Maydis and Saccharomyces Cerevisiae Cell Populations." Fungal Genetics and Biology : FG & B, vol. 134, 2019, p. 103284.
Milisavljevic M, Kojic M. A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations. Fungal Genet Biol. 2019;134:103284.
Milisavljevic, M., & Kojic, M. (2019). A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations. Fungal Genetics and Biology : FG & B, 134, p. 103284. doi:10.1016/j.fgb.2019.103284.
Milisavljevic M, Kojic M. A Comparative Study of Liquid Holding Restitution of Viability After Oxidative Stress in Ustilago Maydis and Saccharomyces Cerevisiae Cell Populations. Fungal Genet Biol. 2019 Oct 18;134:103284. PubMed PMID: 31634563.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations. AU - Milisavljevic,Mira, AU - Kojic,Milorad, Y1 - 2019/10/18/ PY - 2019/08/01/received PY - 2019/10/03/revised PY - 2019/10/15/accepted PY - 2019/10/22/pubmed PY - 2019/10/22/medline PY - 2019/10/22/entrez KW - Adaptation KW - Desiccation KW - Liquid holding KW - Oxidative stress KW - Regrowth SP - 103284 EP - 103284 JF - Fungal genetics and biology : FG & B JO - Fungal Genet. Biol. VL - 134 N2 - The ability of Saccharomyces cerevisiae to reconstitute viability after strong peroxide-induced oxidative stress during liquid holding (LH) in non-nutrient medium has been compared with that of Ustilago maydis. It was found that like U. maydis, S. cerevisiae was capable of reconstituting viability through multiplication of the survivors. However, differences were observed in the pattern of their response: (i) the reconstitution of viability was slower in S. cerevisiae; (ii) before the viability was progressively increasing the treated samples of this fungus reproducibly passed through a phase of additional decrease of the surviving fraction and (iii) the final yields of viable cells attained in S. cerevisiae were below those achieved by U. maydis. The reason for the relative superiority of U. maydis is twofold: (1) early initiated and faster degradation and leakage of the intracellular biomolecules and (2) greater ability of U. maydis cells to recycle damaged and released intracellular compounds. Conceptually similar studies extended to another oxidative-stress-inducing condition, namely desiccation, indicated that the marked differences between these fungi in their patterns of the post-stress regrowth, cellular leakage and macromolecule decomposition are reproduced during LH of desiccated cells. The concordance of the findings obtained upon these two approaches was also corroborated by an analysis of the post-desiccation LH response of U. maydis mutants (adr1, did4, kel1 and tbp1) that were previously identified as defective in post-peroxide LH restitution of viability. We discuss the findings in terms of their possible relevance to the mechanisms of the ecological and evolutionary adaptation of free-living microorganisms to fluctuating and severely inhospitable environments. SN - 1096-0937 UR - https://www.unboundmedicine.com/medline/citation/31634563/A_comparative_study_of_liquid_holding_restitution_of_viability_after_oxidative_stress_in_Ustilago_maydis_and_Saccharomyces_cerevisiae_cell_populations L2 - https://linkinghub.elsevier.com/retrieve/pii/S1087-1845(19)30241-5 DB - PRIME DP - Unbound Medicine ER -