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Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor.
Biomed Pharmacother 2019; 120:109533BP

Abstract

Nasopharyngeal carcinoma (NPC) is a malignant tumor in South China, characterized with high death rate. If untreated, NPC cells will be invasiveness and then spread to other tissues. In clinical practice, however, lack of early effective screening to prevent the NPC development. Therefore, candidate biomarker for detecting NPC is developing urgently. In current study, human NPC data and samples were collected for tests, followed by cell culture study. As results, Epstein-Barr virus (EBV)-based human NPC sections showed increased expressions of heat shock protein 90 (Hsp90), protein kinase B (AKT), and Hsp90 levels were positively expressed than those in cytokeratin 19 (CK19). The clinical data showed unchanged contents of blood cancer markers. In cell line study, Hsp90-treated cells (CNE1, 5-8 F) resulted in promoted cellular growth and proliferation. Additionally, proliferative proteins of cellular extracellular regulated protein kinase (Erk1/2), phospho-Erk1/2 (Thr202+Tyr204), B-cell lymphoma-2 (Bcl-2), AKT, phospho-AKT (Ser473), Ki-67 were up-regulated in Hsp90 treatments, while the apoptotic protease activating factor-1 (Apaf1) were down-regulated. Followed by treatment with Hsp90 inhibitor, the NPC cells exhibited inhibited cellular proliferation and growth, induced cell apoptosis, reduced proliferative proteins of Erk1/2, phospho-Erk1/2 (Thr202+Tyr204), AKT, phospho-AKT (Ser473), Bcl-2, Ki-67, and elevated Apaf1 expression. In conclusion, the current findings obtained from this study demonstrate that Hsp90 effectively promotes cell proliferation through activating carcinomatous ERK1/2, phospho-Erk1/2 (Thr202+Tyr204), AKT, phospho-AKT (Ser473) expressions in NPC cells. Briefly, Hsp90 may be a promising biomarker to screen NPC, including early stage.

Authors+Show Affiliations

Department of Otolaryngology Head and Neck Surgery, The affiliated Hospital of Guilin Medical University, Guilin, Guangxi, PR China.Department of Otolaryngology Head and Neck Surgery, The affiliated Hospital of Guilin Medical University, Guilin, Guangxi, PR China.Department of Otolaryngology Head and Neck Surgery, The affiliated Hospital of Guilin Medical University, Guilin, Guangxi, PR China.Department of Otolaryngology Head and Neck Surgery, The affiliated Hospital of Guilin Medical University, Guilin, Guangxi, PR China.Guangxi Key Laboratory of Tumor Immunology and Microenvironmental Regulation, Guilin Medical University, Guilin, PR China.Guangxi Key Laboratory of Tumor Immunology and Microenvironmental Regulation, Guilin Medical University, Guilin, PR China. Electronic address: college_sumin@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31634779

Citation

Liu, Fangxian, et al. "Clinical and Biological Significances of Heat Shock Protein 90 (Hsp90) in Human Nasopharyngeal Carcinoma Cells and Anti-cancer Effects of Hsp90 Inhibitor." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 120, 2019, p. 109533.
Liu F, Wang L, Yi S, et al. Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor. Biomed Pharmacother. 2019;120:109533.
Liu, F., Wang, L., Yi, S., Liu, Q., Xu, X., & Su, M. (2019). Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 120, p. 109533. doi:10.1016/j.biopha.2019.109533.
Liu F, et al. Clinical and Biological Significances of Heat Shock Protein 90 (Hsp90) in Human Nasopharyngeal Carcinoma Cells and Anti-cancer Effects of Hsp90 Inhibitor. Biomed Pharmacother. 2019;120:109533. PubMed PMID: 31634779.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor. AU - Liu,Fangxian, AU - Wang,Liangliang, AU - Yi,Shijiang, AU - Liu,Qianghe, AU - Xu,Xiaoxiao, AU - Su,Min, Y1 - 2019/10/18/ PY - 2019/06/08/received PY - 2019/10/02/revised PY - 2019/10/02/accepted PY - 2019/10/22/pubmed PY - 2019/10/22/medline PY - 2019/10/22/entrez KW - Heat shock protein 90 KW - Inhibitor KW - Marker KW - Nasopharyngeal carcinoma KW - Proliferation SP - 109533 EP - 109533 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed. Pharmacother. VL - 120 N2 - Nasopharyngeal carcinoma (NPC) is a malignant tumor in South China, characterized with high death rate. If untreated, NPC cells will be invasiveness and then spread to other tissues. In clinical practice, however, lack of early effective screening to prevent the NPC development. Therefore, candidate biomarker for detecting NPC is developing urgently. In current study, human NPC data and samples were collected for tests, followed by cell culture study. As results, Epstein-Barr virus (EBV)-based human NPC sections showed increased expressions of heat shock protein 90 (Hsp90), protein kinase B (AKT), and Hsp90 levels were positively expressed than those in cytokeratin 19 (CK19). The clinical data showed unchanged contents of blood cancer markers. In cell line study, Hsp90-treated cells (CNE1, 5-8 F) resulted in promoted cellular growth and proliferation. Additionally, proliferative proteins of cellular extracellular regulated protein kinase (Erk1/2), phospho-Erk1/2 (Thr202+Tyr204), B-cell lymphoma-2 (Bcl-2), AKT, phospho-AKT (Ser473), Ki-67 were up-regulated in Hsp90 treatments, while the apoptotic protease activating factor-1 (Apaf1) were down-regulated. Followed by treatment with Hsp90 inhibitor, the NPC cells exhibited inhibited cellular proliferation and growth, induced cell apoptosis, reduced proliferative proteins of Erk1/2, phospho-Erk1/2 (Thr202+Tyr204), AKT, phospho-AKT (Ser473), Bcl-2, Ki-67, and elevated Apaf1 expression. In conclusion, the current findings obtained from this study demonstrate that Hsp90 effectively promotes cell proliferation through activating carcinomatous ERK1/2, phospho-Erk1/2 (Thr202+Tyr204), AKT, phospho-AKT (Ser473) expressions in NPC cells. Briefly, Hsp90 may be a promising biomarker to screen NPC, including early stage. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/31634779/Clinical_and_biological_significances_of_heat_shock_protein_90_(Hsp90)_in_human_nasopharyngeal_carcinoma_cells_and_anti-cancer_effects_of_Hsp90_inhibitor L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(19)32593-4 DB - PRIME DP - Unbound Medicine ER -