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Untargeted metabolomic profiling reveals that different responses to self and cross pollination in each flower morph of the heteromorphic plant Plumbago auriculata.
Plant Physiol Biochem 2019; 144:413-426PP

Abstract

Heteromorphic self-incompatibility (HetSI), which is regulated by sporophytes, occurs in some species as a strategy to promote cross-pollination. This research aimed to reveal metabolic changes occurring in HetSI. We used fluorescence microscopy as a tool to compare growth behavior in self-incompatible (SI) and self-compatible (SC) pollination in both pin and thrum flowers of Plumbago auriculata and to identify the ideal timepoint for sample collection for subsequent experiments. We also employed scanning electron microscopy (SEM) to evaluate intermorph structural differences in the pollen grains and stigmas in relation to HetSI. Importantly, UPLC-MS/MS was applied in this study to identify metabolites, compare metabolic differences between pin and thrum styles and monitor metabolic changes in SC and SI pollinations in the two types of flowers. The metabolites mainly included amino acids/peptides, flavonoids, glycosides/sugars, phenols, other organic acids, fatty acids (derivatives)/lipids, amines, aldehydes, alkaloids, alcohols and other compounds. Surprisingly, energy-related nutrients such as amino acids/peptides and tricarboxylic acid cycle-related metabolites were found at higher levels in SI pollinations than in SC pollinations. This result indicates that physiological changes in pollen-stigma interactions differ in pin and thrum styles and SC and SI pollinations and that energy deficiency is not one of the reasons for HetSI.

Authors+Show Affiliations

Landscape Research Institute, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China. Electronic address: gailhu@163.com.Landscape Research Institute, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China. Electronic address: liwenjilwj@126.com.Landscape Research Institute, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China. Electronic address: gao_suping@sicau.edu.cn.Landscape Research Institute, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China. Electronic address: ting_lei85@sicau.edu.cn.Landscape Research Institute, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China. Electronic address: huju918@yahoo.com.Landscape Research Institute, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China. Electronic address: 18428385614@163.com.Landscape Research Institute, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China. Electronic address: yellor326815@163.com.Landscape Research Institute, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China. Electronic address: zaq135@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31634809

Citation

Hu, Di, et al. "Untargeted Metabolomic Profiling Reveals That Different Responses to Self and Cross Pollination in Each Flower Morph of the Heteromorphic Plant Plumbago Auriculata." Plant Physiology and Biochemistry : PPB, vol. 144, 2019, pp. 413-426.
Hu D, Li W, Gao S, et al. Untargeted metabolomic profiling reveals that different responses to self and cross pollination in each flower morph of the heteromorphic plant Plumbago auriculata. Plant Physiol Biochem. 2019;144:413-426.
Hu, D., Li, W., Gao, S., Lei, T., Hu, J., Shen, P., ... Li, J. (2019). Untargeted metabolomic profiling reveals that different responses to self and cross pollination in each flower morph of the heteromorphic plant Plumbago auriculata. Plant Physiology and Biochemistry : PPB, 144, pp. 413-426. doi:10.1016/j.plaphy.2019.10.010.
Hu D, et al. Untargeted Metabolomic Profiling Reveals That Different Responses to Self and Cross Pollination in Each Flower Morph of the Heteromorphic Plant Plumbago Auriculata. Plant Physiol Biochem. 2019;144:413-426. PubMed PMID: 31634809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Untargeted metabolomic profiling reveals that different responses to self and cross pollination in each flower morph of the heteromorphic plant Plumbago auriculata. AU - Hu,Di, AU - Li,Wenji, AU - Gao,Suping, AU - Lei,Ting, AU - Hu,Ju, AU - Shen,Ping, AU - Li,Yurong, AU - Li,Jiani, Y1 - 2019/10/12/ PY - 2019/07/22/received PY - 2019/10/08/revised PY - 2019/10/08/accepted PY - 2019/10/22/pubmed PY - 2019/10/22/medline PY - 2019/10/22/entrez KW - Fluorescence microscopy KW - HetSI KW - Non-targeted metabolomic analysis KW - Plumbago auriculata lam KW - Pollen-stigma interaction KW - Scanning electron microscopy KW - UPLC-MS/MS SP - 413 EP - 426 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol. Biochem. VL - 144 N2 - Heteromorphic self-incompatibility (HetSI), which is regulated by sporophytes, occurs in some species as a strategy to promote cross-pollination. This research aimed to reveal metabolic changes occurring in HetSI. We used fluorescence microscopy as a tool to compare growth behavior in self-incompatible (SI) and self-compatible (SC) pollination in both pin and thrum flowers of Plumbago auriculata and to identify the ideal timepoint for sample collection for subsequent experiments. We also employed scanning electron microscopy (SEM) to evaluate intermorph structural differences in the pollen grains and stigmas in relation to HetSI. Importantly, UPLC-MS/MS was applied in this study to identify metabolites, compare metabolic differences between pin and thrum styles and monitor metabolic changes in SC and SI pollinations in the two types of flowers. The metabolites mainly included amino acids/peptides, flavonoids, glycosides/sugars, phenols, other organic acids, fatty acids (derivatives)/lipids, amines, aldehydes, alkaloids, alcohols and other compounds. Surprisingly, energy-related nutrients such as amino acids/peptides and tricarboxylic acid cycle-related metabolites were found at higher levels in SI pollinations than in SC pollinations. This result indicates that physiological changes in pollen-stigma interactions differ in pin and thrum styles and SC and SI pollinations and that energy deficiency is not one of the reasons for HetSI. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/31634809/Untargeted_metabolomic_profiling_reveals_that_different_responses_to_self_and_cross_pollination_in_each_flower_morph_of_the_heteromorphic_plant_Plumbago_auriculata L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(19)30411-5 DB - PRIME DP - Unbound Medicine ER -