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Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering.
Int J Mol Sci. 2019 Oct 18; 20(20)IJ

Abstract

Aloe vera (AV) and tetracycline hydrochloride (TCH) exhibit significant properties such as anti-inflammatory, antioxidant and anti-bacterial activities to facilitate skin tissue engineering. The present study aims to develop poly-ε-caprolactone (PCL)/ AV containing curcumin (CUR), and TCH loaded hybrid nanofibrous scaffolds to validate the synergistic effect on the fibroblast proliferation and antimicrobial activity against Gram-positive and Gram-negative bacteria for wound healing. PCL/AV, PCL/CUR, PCL/AV/CUR and PCL/AV/TCH hybrid nanofibrous mats were fabricated using an electrospinning technique and were characterized for surface morphology, the successful incorporation of active compounds, hydrophilicity and the mechanical property of nanofibers. SEM revealed that there was a decrease in the fiber diameter (ranging from 360 to 770 nm) upon the addition of AV, CUR and TCH in PCL nanofibers, which were randomly oriented with bead free morphology. FTIR spectra of various electrospun samples confirmed the successful incorporation of AV, CUR and TCH into the PCL nanofibers. The fabricated nanofibrous scaffolds possessed mechanical properties within the range of human skin. The biocompatibility of electrospun nanofibrous scaffolds were evaluated on primary human dermal fibroblasts (hDF) by MTS assay, CMFDA, Sirius red and F-actin stainings. The results showed that the fabricated PCL/AV/CUR and PCL/AV/TCH nanofibrous scaffolds were non-toxic and had the potential for wound healing applications. The disc diffusion assay confirmed that the electrospun nanofibrous scaffolds possessed antibacterial activity and provided an effective wound dressing for skin tissue engineering.

Authors+Show Affiliations

Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117594, Singapore. anthe@nus.edu.sg. Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore. anthe@nus.edu.sg.Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore. raghavendra8025@gmail.com.Anti-Infectives Research Group, Singapore Eye Research Institute, Singapore 169856, Singapore. Chetnachem24@gmail.com.Anti-Infectives Research Group, Singapore Eye Research Institute, Singapore 169856, Singapore. lakshminarayanan.rajamani@seri.com.sg.Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore. drancer.asif@gmail.com.Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore. gandhi@tll.org.sg.Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore. seeram@nus.edu.sg.Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117594, Singapore. antbaybh@nus.edu.sg.Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore. jrvgopal@gmail.com. Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Gambang 26300, Malaysia. jrvgopal@gmail.com.Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117594, Singapore. dineshkumar@nus.edu.sg.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31635374

Citation

Ezhilarasu, Hariharan, et al. "Biocompatible Aloe Vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering." International Journal of Molecular Sciences, vol. 20, no. 20, 2019.
Ezhilarasu H, Ramalingam R, Dhand C, et al. Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering. Int J Mol Sci. 2019;20(20).
Ezhilarasu, H., Ramalingam, R., Dhand, C., Lakshminarayanan, R., Sadiq, A., Gandhimathi, C., Ramakrishna, S., Bay, B. H., Venugopal, J. R., & Srinivasan, D. K. (2019). Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering. International Journal of Molecular Sciences, 20(20). https://doi.org/10.3390/ijms20205174
Ezhilarasu H, et al. Biocompatible Aloe Vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering. Int J Mol Sci. 2019 Oct 18;20(20) PubMed PMID: 31635374.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering. AU - Ezhilarasu,Hariharan, AU - Ramalingam,Raghavendra, AU - Dhand,Chetna, AU - Lakshminarayanan,Rajamani, AU - Sadiq,Asif, AU - Gandhimathi,Chinnasamy, AU - Ramakrishna,Seeram, AU - Bay,Boon Huat, AU - Venugopal,Jayarama Reddy, AU - Srinivasan,Dinesh Kumar, Y1 - 2019/10/18/ PY - 2019/09/20/received PY - 2019/10/16/revised PY - 2019/10/16/accepted PY - 2019/10/23/entrez PY - 2019/10/23/pubmed PY - 2019/10/23/medline KW - aloe vera KW - curcumin KW - electrospinning KW - fibroblasts KW - nanofibers KW - tetracycline hydrochloride KW - wound healing JF - International journal of molecular sciences JO - Int J Mol Sci VL - 20 IS - 20 N2 - Aloe vera (AV) and tetracycline hydrochloride (TCH) exhibit significant properties such as anti-inflammatory, antioxidant and anti-bacterial activities to facilitate skin tissue engineering. The present study aims to develop poly-ε-caprolactone (PCL)/ AV containing curcumin (CUR), and TCH loaded hybrid nanofibrous scaffolds to validate the synergistic effect on the fibroblast proliferation and antimicrobial activity against Gram-positive and Gram-negative bacteria for wound healing. PCL/AV, PCL/CUR, PCL/AV/CUR and PCL/AV/TCH hybrid nanofibrous mats were fabricated using an electrospinning technique and were characterized for surface morphology, the successful incorporation of active compounds, hydrophilicity and the mechanical property of nanofibers. SEM revealed that there was a decrease in the fiber diameter (ranging from 360 to 770 nm) upon the addition of AV, CUR and TCH in PCL nanofibers, which were randomly oriented with bead free morphology. FTIR spectra of various electrospun samples confirmed the successful incorporation of AV, CUR and TCH into the PCL nanofibers. The fabricated nanofibrous scaffolds possessed mechanical properties within the range of human skin. The biocompatibility of electrospun nanofibrous scaffolds were evaluated on primary human dermal fibroblasts (hDF) by MTS assay, CMFDA, Sirius red and F-actin stainings. The results showed that the fabricated PCL/AV/CUR and PCL/AV/TCH nanofibrous scaffolds were non-toxic and had the potential for wound healing applications. The disc diffusion assay confirmed that the electrospun nanofibrous scaffolds possessed antibacterial activity and provided an effective wound dressing for skin tissue engineering. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/31635374/Biocompatible_Aloe_vera_and_Tetracycline_Hydrochloride_Loaded_Hybrid_Nanofibrous_Scaffolds_for_Skin_Tissue_Engineering_ L2 - https://www.mdpi.com/resolver?pii=ijms20205174 DB - PRIME DP - Unbound Medicine ER -
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