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Denitrifying Microbial Community Structure and bamA Gene Diversity of Phenol Degraders in Soil Contaminated from the Coking Process.

Abstract

Phenolic compounds are the dominant pollutants in soils contaminated by the coking industry. Ring opening by the hydroxylase gene (bamA) is the key step in the benzoyl-CoA degradation pathway under anaerobic conditions, and a broad spectrum of microorganisms possesses this functional gene, including denitrifiers. The present study analyzed the community structure of denitrifying bacteria and the diversity of the bamA gene for mixed cultures enriched from soil collected at a coking industrial site and then grown under nitrate-reducing conditions on phenol or p-hydroxybenzoate (4HBA), a key intermediate product of anaerobic phenol degradation. Illumina sequencing of the 16S rRNA gene showed different bacterial compositions between the two cultures. The dominant phyla were Proteobacteria, Armatimonadetes, and Planctomycetes in the phenol culture and Proteobacteria and Bacteroidetes in the 4HBA culture. Phylogenetic analysis further demonstrated that bamA genes were associated with four clusters of bacteria, three of known bacteria and one of uncultured bacteria. The diversity of the bamA gene differed from that reported in anaerobic aromatic degradation cultures, suggesting that these enriched cultures may contain new strains unique to coking-contaminated soils. The present study further validates the potential application of this functional gene as a marker for anaerobic biodegradation processes in enrichment cultures from contaminated soil.

Authors+Show Affiliations

College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, China. wangguoying@tyut.edu.cn.College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.Department of Environmental Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, 08901, USA.Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Sciences & Technology, Guangzhou, 510650, China. Department of Environmental Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, 08901, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31637583

Citation

Li, Yanan, et al. "Denitrifying Microbial Community Structure and bamA Gene Diversity of Phenol Degraders in Soil Contaminated From the Coking Process." Applied Biochemistry and Biotechnology, 2019.
Li Y, Li J, Wang D, et al. Denitrifying Microbial Community Structure and bamA Gene Diversity of Phenol Degraders in Soil Contaminated from the Coking Process. Appl Biochem Biotechnol. 2019.
Li, Y., Li, J., Wang, D., Wang, G., Yue, X., Kong, X., ... Huang, W. (2019). Denitrifying Microbial Community Structure and bamA Gene Diversity of Phenol Degraders in Soil Contaminated from the Coking Process. Applied Biochemistry and Biotechnology, doi:10.1007/s12010-019-03144-5.
Li Y, et al. Denitrifying Microbial Community Structure and bamA Gene Diversity of Phenol Degraders in Soil Contaminated From the Coking Process. Appl Biochem Biotechnol. 2019 Oct 22; PubMed PMID: 31637583.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Denitrifying Microbial Community Structure and bamA Gene Diversity of Phenol Degraders in Soil Contaminated from the Coking Process. AU - Li,Yanan, AU - Li,Jing, AU - Wang,Di, AU - Wang,Guoying, AU - Yue,Xiuping, AU - Kong,Xin, AU - Young,Lily, AU - Huang,Weilin, Y1 - 2019/10/22/ PY - 2019/03/11/received PY - 2019/09/12/accepted PY - 2019/10/23/entrez KW - Azoarcus KW - Denitrifiers KW - Illumina sequencing KW - Phenol KW - bamA gene KW - p-Hydroxybenzoate JF - Applied biochemistry and biotechnology JO - Appl. Biochem. Biotechnol. N2 - Phenolic compounds are the dominant pollutants in soils contaminated by the coking industry. Ring opening by the hydroxylase gene (bamA) is the key step in the benzoyl-CoA degradation pathway under anaerobic conditions, and a broad spectrum of microorganisms possesses this functional gene, including denitrifiers. The present study analyzed the community structure of denitrifying bacteria and the diversity of the bamA gene for mixed cultures enriched from soil collected at a coking industrial site and then grown under nitrate-reducing conditions on phenol or p-hydroxybenzoate (4HBA), a key intermediate product of anaerobic phenol degradation. Illumina sequencing of the 16S rRNA gene showed different bacterial compositions between the two cultures. The dominant phyla were Proteobacteria, Armatimonadetes, and Planctomycetes in the phenol culture and Proteobacteria and Bacteroidetes in the 4HBA culture. Phylogenetic analysis further demonstrated that bamA genes were associated with four clusters of bacteria, three of known bacteria and one of uncultured bacteria. The diversity of the bamA gene differed from that reported in anaerobic aromatic degradation cultures, suggesting that these enriched cultures may contain new strains unique to coking-contaminated soils. The present study further validates the potential application of this functional gene as a marker for anaerobic biodegradation processes in enrichment cultures from contaminated soil. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/31637583/Denitrifying_Microbial_Community_Structure_and_bamA_Gene_Diversity_of_Phenol_Degraders_in_Soil_Contaminated_from_the_Coking_Process L2 - https://dx.doi.org/10.1007/s12010-019-03144-5 DB - PRIME DP - Unbound Medicine ER -