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Sulfurimonas crateris sp. nov., a facultative anaerobic sulfur-oxidizing chemolithoautotrophic bacterium isolated from a terrestrial mud volcano.

Abstract

A novel mesophilic facultative anaerobic bacterium, strain SN118T, was isolated from a terrestrial mud volcano in Taman Peninsula, Russia. The cells were Gram-negative, motile, short, straight or curved rods with a single polar flagellum. Growth was observed at 5-40 °C (optimum, 30 °C) and pH 5.5-9.5 (optimum, pH 8.0). Growth of strain SN118T was observed in NaCl concentrations ranging from 0.5 to 8.0 % (w/v) with an optimum at 2.0-3.0 % (w/v). The isolate grew chemolithoautotrophically with sulfide, elemental sulfur or thiosulfate as electron donor, oxygen, nitrate or nitrite as an electron acceptor and CO2/HCO3 - as a carbon source. Molecular hydrogen or organic substances did not support growth. Nitrate was reduced to N2. The dominant fatty acids were C16 : 1ω7c, C16 : 0 and C18 : 1ω7c. The total size of the genome of the novel isolate was 2 209 279 bp and the genomic DNA G+C content was 38.8 mol%. Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolate belonged to the genus Sulfurimonas and was most closely related to Sulfurimonas denitrificans DSM 1251T (96.74 %). Based on its physiological properties and results from phylogenetic analyses, including average nucleotide identity and in silico DNA-DNA hybridization values, the isolate is considered to represent a novel species of the genus Sulfurimonas, for which the name Sulfurimonas crateris sp. nov. is proposed. The type strain is SN118T (=DSM 109248T=VKM B-3378T).

Authors+Show Affiliations

Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect, 33, bld. 2, 119071, Moscow, Russia.Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect, 33, bld. 2, 119071, Moscow, Russia.Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect, 33, bld. 2, 119071, Moscow, Russia.Gubkin University, Leninsky Prospect, 65/1, 119991, Moscow, Russia.Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect, 33, bld. 2, 119071, Moscow, Russia.Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect, 33, bld. 2, 119071, Moscow, Russia.Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect, 33, bld. 2, 119071, Moscow, Russia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31639074

Citation

Ratnikova, N M., et al. "Sulfurimonas Crateris Sp. Nov., a Facultative Anaerobic Sulfur-oxidizing Chemolithoautotrophic Bacterium Isolated From a Terrestrial Mud Volcano." International Journal of Systematic and Evolutionary Microbiology, 2019.
Ratnikova NM, Slobodkin AI, Merkel AY, et al. Sulfurimonas crateris sp. nov., a facultative anaerobic sulfur-oxidizing chemolithoautotrophic bacterium isolated from a terrestrial mud volcano. Int J Syst Evol Microbiol. 2019.
Ratnikova, N. M., Slobodkin, A. I., Merkel, A. Y., Kopitsyn, D. S., Kevbrin, V. V., Bonch-Osmolovskaya, E. A., & Slobodkina, G. B. (2019). Sulfurimonas crateris sp. nov., a facultative anaerobic sulfur-oxidizing chemolithoautotrophic bacterium isolated from a terrestrial mud volcano. International Journal of Systematic and Evolutionary Microbiology, doi:10.1099/ijsem.0.003779.
Ratnikova NM, et al. Sulfurimonas Crateris Sp. Nov., a Facultative Anaerobic Sulfur-oxidizing Chemolithoautotrophic Bacterium Isolated From a Terrestrial Mud Volcano. Int J Syst Evol Microbiol. 2019 Oct 22; PubMed PMID: 31639074.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sulfurimonas crateris sp. nov., a facultative anaerobic sulfur-oxidizing chemolithoautotrophic bacterium isolated from a terrestrial mud volcano. AU - Ratnikova,N M, AU - Slobodkin,A I, AU - Merkel,A Y, AU - Kopitsyn,D S, AU - Kevbrin,V V, AU - Bonch-Osmolovskaya,E A, AU - Slobodkina,G B, Y1 - 2019/10/22/ PY - 2019/10/23/entrez PY - 2019/10/23/pubmed PY - 2019/10/23/medline KW - CO2 fixation KW - Epsilonproteobacteria KW - Sox pathway KW - denitrification JF - International journal of systematic and evolutionary microbiology JO - Int. J. Syst. Evol. Microbiol. N2 - A novel mesophilic facultative anaerobic bacterium, strain SN118T, was isolated from a terrestrial mud volcano in Taman Peninsula, Russia. The cells were Gram-negative, motile, short, straight or curved rods with a single polar flagellum. Growth was observed at 5-40 °C (optimum, 30 °C) and pH 5.5-9.5 (optimum, pH 8.0). Growth of strain SN118T was observed in NaCl concentrations ranging from 0.5 to 8.0 % (w/v) with an optimum at 2.0-3.0 % (w/v). The isolate grew chemolithoautotrophically with sulfide, elemental sulfur or thiosulfate as electron donor, oxygen, nitrate or nitrite as an electron acceptor and CO2/HCO3 - as a carbon source. Molecular hydrogen or organic substances did not support growth. Nitrate was reduced to N2. The dominant fatty acids were C16 : 1ω7c, C16 : 0 and C18 : 1ω7c. The total size of the genome of the novel isolate was 2 209 279 bp and the genomic DNA G+C content was 38.8 mol%. Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolate belonged to the genus Sulfurimonas and was most closely related to Sulfurimonas denitrificans DSM 1251T (96.74 %). Based on its physiological properties and results from phylogenetic analyses, including average nucleotide identity and in silico DNA-DNA hybridization values, the isolate is considered to represent a novel species of the genus Sulfurimonas, for which the name Sulfurimonas crateris sp. nov. is proposed. The type strain is SN118T (=DSM 109248T=VKM B-3378T). SN - 1466-5034 UR - https://www.unboundmedicine.com/medline/citation/31639074/Sulfurimonas_crateris_sp._nov.,_a_facultative_anaerobic_sulfur-oxidizing_chemolithoautotrophic_bacterium_isolated_from_a_terrestrial_mud_volcano DB - PRIME DP - Unbound Medicine ER -