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Recentrifugation of Lithium Heparin Gel Separator Tubes up to 8 h after Blood Collection Has No Relevant Influence on the Stability of 30 Routine Biochemical Analytes.
J Appl Lab Med 2019; 3(5):864-869JA

Abstract

BACKGROUND

Venipuncture for the purpose of blood analysis is often performed at remote locations, and samples may be centrifuged locally to preserve the integrity of analytes. At the central laboratory, these tubes may be centrifuged again in the routine process. However, limited research shows that >1 centrifugation cycle of gel separator tubes causes significant changes in analytes, in particular troponin I and potassium. These preanalytical test changes are undesirable and may lead to errors in diagnosis and treatment of patients.

METHODS

Ten volunteers donated blood in 10 lithium heparin gel tubes. Per volunteer, 5 tubes were centrifuged with Becton Dickinson centrifugation settings and 5 tubes with our local centrifugation settings. For each centrifugation setting, 1 tube was centrifuged directly after venipuncture; the second tube, directly after venipuncture and again after 4 h; the third tube, directly after venipuncture and again after 8 h; the fourth tube, 4 h after venipuncture; the last tube, 8 h after venipuncture. Thirty routine chemistry analyses were performed in plasma directly after the last centrifugation cycle. All tubes were kept at room temperature. Analytes were considered unstable when the mean percentage deviation exceeded the total allowable error.

RESULTS

Except for calcium, which slightly exceeded the predefined total allowable error limit, all the investigated analytes remained stable up to 8 h after a second centrifugation cycle with both centrifugation settings.

CONCLUSION

This study shows that recentrifugation up to 8 h after blood collection does not cause relevant deviations in test results and may be applied safely.

Authors+Show Affiliations

Laboratory for Clinical Chemistry and Haematology, Jeroen Bosch Hospital, 's-Hertogenbosch, the Netherlands; j.v.balveren@jbz.nl.Laboratory for Clinical Chemistry and Haematology, Jeroen Bosch Hospital, 's-Hertogenbosch, the Netherlands.Laboratory for Clinical Chemistry and Haematology, Jeroen Bosch Hospital, 's-Hertogenbosch, the Netherlands. Department of Health Technology and Services Research, MIRA Institute of Biomedical Technology and Technical Medicine, University of Twente, Enschede, the Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31639761

Citation

van Balveren, Jasmijn A., et al. "Recentrifugation of Lithium Heparin Gel Separator Tubes Up to 8 H After Blood Collection Has No Relevant Influence On the Stability of 30 Routine Biochemical Analytes." The Journal of Applied Laboratory Medicine, vol. 3, no. 5, 2019, pp. 864-869.
van Balveren JA, Gemen EFA, Kusters R. Recentrifugation of Lithium Heparin Gel Separator Tubes up to 8 h after Blood Collection Has No Relevant Influence on the Stability of 30 Routine Biochemical Analytes. J Appl Lab Med. 2019;3(5):864-869.
van Balveren, J. A., Gemen, E. F. A., & Kusters, R. (2019). Recentrifugation of Lithium Heparin Gel Separator Tubes up to 8 h after Blood Collection Has No Relevant Influence on the Stability of 30 Routine Biochemical Analytes. The Journal of Applied Laboratory Medicine, 3(5), pp. 864-869. doi:10.1373/jalm.2018.026567.
van Balveren JA, Gemen EFA, Kusters R. Recentrifugation of Lithium Heparin Gel Separator Tubes Up to 8 H After Blood Collection Has No Relevant Influence On the Stability of 30 Routine Biochemical Analytes. J Appl Lab Med. 2019;3(5):864-869. PubMed PMID: 31639761.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recentrifugation of Lithium Heparin Gel Separator Tubes up to 8 h after Blood Collection Has No Relevant Influence on the Stability of 30 Routine Biochemical Analytes. AU - van Balveren,Jasmijn A, AU - Gemen,Eugenie F A, AU - Kusters,Ron, Y1 - 2018/05/24/ PY - 2018/03/13/received PY - 2018/05/04/accepted PY - 2019/10/23/entrez PY - 2019/10/23/pubmed PY - 2019/10/23/medline SP - 864 EP - 869 JF - The journal of applied laboratory medicine JO - J Appl Lab Med VL - 3 IS - 5 N2 - BACKGROUND: Venipuncture for the purpose of blood analysis is often performed at remote locations, and samples may be centrifuged locally to preserve the integrity of analytes. At the central laboratory, these tubes may be centrifuged again in the routine process. However, limited research shows that >1 centrifugation cycle of gel separator tubes causes significant changes in analytes, in particular troponin I and potassium. These preanalytical test changes are undesirable and may lead to errors in diagnosis and treatment of patients. METHODS: Ten volunteers donated blood in 10 lithium heparin gel tubes. Per volunteer, 5 tubes were centrifuged with Becton Dickinson centrifugation settings and 5 tubes with our local centrifugation settings. For each centrifugation setting, 1 tube was centrifuged directly after venipuncture; the second tube, directly after venipuncture and again after 4 h; the third tube, directly after venipuncture and again after 8 h; the fourth tube, 4 h after venipuncture; the last tube, 8 h after venipuncture. Thirty routine chemistry analyses were performed in plasma directly after the last centrifugation cycle. All tubes were kept at room temperature. Analytes were considered unstable when the mean percentage deviation exceeded the total allowable error. RESULTS: Except for calcium, which slightly exceeded the predefined total allowable error limit, all the investigated analytes remained stable up to 8 h after a second centrifugation cycle with both centrifugation settings. CONCLUSION: This study shows that recentrifugation up to 8 h after blood collection does not cause relevant deviations in test results and may be applied safely. SN - 2475-7241 UR - https://www.unboundmedicine.com/medline/citation/31639761/Recentrifugation_of_Lithium_Heparin_Gel_Separator_Tubes_up_to_8_h_after_Blood_Collection_Has_No_Relevant_Influence_on_the_Stability_of_30_Routine_Biochemical_Analytes L2 - http://jalm.aaccjnls.org/cgi/pmidlookup?view=long&pmid=31639761 DB - PRIME DP - Unbound Medicine ER -