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Phenotypic and molecular characterization of Hessian fly resistance in diploid wheat, Aegilops tauschii.
BMC Plant Biol. 2019 Oct 22; 19(1):439.BP

Abstract

BACKGROUND

The Hessian fly (Mayetiola destructor), belonging to the gall midge family (Cecidomyiidae), is a devastating pest of wheat (Triticum aestivum) causing significant yield losses. Despite identification and characterization of numerous Hessian fly-responsive genes and associated biological pathways involved in wheat defense against this dipteran pest, their functional validation has been challenging. This is largely attributed to the large genome, polyploidy, repetitive DNA, and limited genetic resources in hexaploid wheat. The diploid progenitor Aegilops tauschii, D-genome donor of modern-day hexaploid wheat, offers an ideal surrogate eliminating the need to target all three homeologous chromosomes (A, B and D) individually, and thereby making the functional validation of candidate Hessian fly-responsive genes plausible. Furthermore, the well-annotated sequence of Ae. tauschii genome and availability of genetic resources amenable to manipulations makes the functional assays less tedious and time-consuming. However, prior to utilization of this diploid genome for downstream studies, it is imperative to characterize its physical and molecular responses to Hessian fly.

RESULTS

In this study we screened five Ae. tauschii accessions for their response to the Hessian fly biotypes L and vH13. Two lines were identified that exhibited a homozygous resistance response to feeding by both Hessian fly biotypes. Studies using physical measurements and neutral red staining showed that the resistant Ae. tauschii accessions resembled hexaploid wheat in their phenotypic responses to Hessian fly, that included similarities in larval developmental stages, leaf and plant growth, and cell wall permeability. Furthermore, molecular responses, characterized by gene expression profiling using quantitative real-time PCR, in select resistant Ae. tauschii lines also revealed similarities with resistant hexaploid wheat.

CONCLUSIONS

Phenotypic and molecular characterization of Ae. tauschii to Hessian fly infestation revealed resistant accessions that shared similarities to hexaploid wheat. Resembling the resistant hexaploid wheat, the Ae. tauschii accessions mount an early defense strategy involving defense proteins including lectins, secondary metabolites and reactive oxygen species (ROS) radicals. Our results reveal the suitability of the diploid progenitor for use as an ideal tool for functional genomics research in deciphering the wheat-Hessian fly molecular interactions.

Authors+Show Affiliations

USDA-ARS Crop Production and Pest Control Research Unit, West Lafayette, IN, 47907, USA. Department of Entomology, Purdue University, West Lafayette, IN, 47907, USA.USDA-ARS Crop Production and Pest Control Research Unit, West Lafayette, IN, 47907, USA. Department of Entomology, Purdue University, West Lafayette, IN, 47907, USA.USDA-ARS Crop Production and Pest Control Research Unit, West Lafayette, IN, 47907, USA. Department of Agronomy, Purdue University, West Lafayette, IN, 47907, USA.USDA-ARS Crop Production and Pest Control Research Unit, West Lafayette, IN, 47907, USA. subhashree.subramanyam@usda.gov. Department of Agronomy, Purdue University, West Lafayette, IN, 47907, USA. subhashree.subramanyam@usda.gov.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31640550

Citation

Nemacheck, Jill A., et al. "Phenotypic and Molecular Characterization of Hessian Fly Resistance in Diploid Wheat, Aegilops Tauschii." BMC Plant Biology, vol. 19, no. 1, 2019, p. 439.
Nemacheck JA, Schemerhorn BJ, Scofield SR, et al. Phenotypic and molecular characterization of Hessian fly resistance in diploid wheat, Aegilops tauschii. BMC Plant Biol. 2019;19(1):439.
Nemacheck, J. A., Schemerhorn, B. J., Scofield, S. R., & Subramanyam, S. (2019). Phenotypic and molecular characterization of Hessian fly resistance in diploid wheat, Aegilops tauschii. BMC Plant Biology, 19(1), 439. https://doi.org/10.1186/s12870-019-2058-6
Nemacheck JA, et al. Phenotypic and Molecular Characterization of Hessian Fly Resistance in Diploid Wheat, Aegilops Tauschii. BMC Plant Biol. 2019 Oct 22;19(1):439. PubMed PMID: 31640550.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic and molecular characterization of Hessian fly resistance in diploid wheat, Aegilops tauschii. AU - Nemacheck,Jill A, AU - Schemerhorn,Brandon J, AU - Scofield,Steven R, AU - Subramanyam,Subhashree, Y1 - 2019/10/22/ PY - 2019/06/24/received PY - 2019/09/27/accepted PY - 2019/10/24/entrez PY - 2019/10/24/pubmed PY - 2020/1/28/medline KW - Biotic stress KW - Functional genomics KW - Insect resistance KW - Lectins KW - Oxidative stress KW - Permeability KW - Secondary metabolites KW - Surrogate KW - qRT-PCR SP - 439 EP - 439 JF - BMC plant biology JO - BMC Plant Biol VL - 19 IS - 1 N2 - BACKGROUND: The Hessian fly (Mayetiola destructor), belonging to the gall midge family (Cecidomyiidae), is a devastating pest of wheat (Triticum aestivum) causing significant yield losses. Despite identification and characterization of numerous Hessian fly-responsive genes and associated biological pathways involved in wheat defense against this dipteran pest, their functional validation has been challenging. This is largely attributed to the large genome, polyploidy, repetitive DNA, and limited genetic resources in hexaploid wheat. The diploid progenitor Aegilops tauschii, D-genome donor of modern-day hexaploid wheat, offers an ideal surrogate eliminating the need to target all three homeologous chromosomes (A, B and D) individually, and thereby making the functional validation of candidate Hessian fly-responsive genes plausible. Furthermore, the well-annotated sequence of Ae. tauschii genome and availability of genetic resources amenable to manipulations makes the functional assays less tedious and time-consuming. However, prior to utilization of this diploid genome for downstream studies, it is imperative to characterize its physical and molecular responses to Hessian fly. RESULTS: In this study we screened five Ae. tauschii accessions for their response to the Hessian fly biotypes L and vH13. Two lines were identified that exhibited a homozygous resistance response to feeding by both Hessian fly biotypes. Studies using physical measurements and neutral red staining showed that the resistant Ae. tauschii accessions resembled hexaploid wheat in their phenotypic responses to Hessian fly, that included similarities in larval developmental stages, leaf and plant growth, and cell wall permeability. Furthermore, molecular responses, characterized by gene expression profiling using quantitative real-time PCR, in select resistant Ae. tauschii lines also revealed similarities with resistant hexaploid wheat. CONCLUSIONS: Phenotypic and molecular characterization of Ae. tauschii to Hessian fly infestation revealed resistant accessions that shared similarities to hexaploid wheat. Resembling the resistant hexaploid wheat, the Ae. tauschii accessions mount an early defense strategy involving defense proteins including lectins, secondary metabolites and reactive oxygen species (ROS) radicals. Our results reveal the suitability of the diploid progenitor for use as an ideal tool for functional genomics research in deciphering the wheat-Hessian fly molecular interactions. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/31640550/Phenotypic_and_molecular_characterization_of_Hessian_fly_resistance_in_diploid_wheat_Aegilops_tauschii_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-2058-6 DB - PRIME DP - Unbound Medicine ER -