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RETRACTED ARTICLE

The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas.
Oxid Med Cell Longev. 2019; 2019:1857086.OM

Abstract

Our previous research revealed that trehalose, a nonreducing disaccharide of glucose and an important stress responsive factor, proved to have anti-inflammatory, antiapoptotic, and particularly antioxidant properties in UVB-irradiated corneas. Trehalose reduced oxidative stress in corneas induced by UVB irradiation, by means of a decrease in the antioxidant/prooxidant imbalance in the corneal epithelium. In this study, we demonstrate that trehalose of 3% or 6% concentration in eye drops directly decreases oxidative stress in UVB-irradiated corneas, by removing the excessive amount of reactive oxygen species (ROS). Trehalose drops applied on corneas during UVB irradiation once daily for four days resulted in a reduction or even absence of the oxidative stress, DNA damage, and peroxynitrite formation (detected by nitrotyrosine residues), seen in buffer-treated corneas. Furthermore, trehalose treatment applied curatively after repeated irradiation for the subsequent fourteen days led to the renewal of corneal transparency and significant suppression or even absence of neovascularization. This was in contrast to buffer-treated irradiated corneas, where the intracorneal inflammation was developed and the untransparent corneas were vascularized. In conclusion, the treatment of UVB-irradiated corneas with trehalose eye drops removed the excessive amount of ROS in the corneal epithelium, leading to the suppression of oxidative stress and favorable corneal healing. The 6% trehalose showed a higher intensive antioxidant effect.

Authors+Show Affiliations

Institute of Experimental Medicine of the Czech Academy of Sciences, Vídenska 1083, 14220 Prague 4, Czech Republic.Institute of Experimental Medicine of the Czech Academy of Sciences, Vídenska 1083, 14220 Prague 4, Czech Republic. Faculty of Natural Science, Charles University, Vinicna 7, 12843 Prague 2, Czech Republic.Institute of Experimental Medicine of the Czech Academy of Sciences, Vídenska 1083, 14220 Prague 4, Czech Republic. Faculty of Natural Science, Charles University, Vinicna 7, 12843 Prague 2, Czech Republic.Institute of Experimental Medicine of the Czech Academy of Sciences, Vídenska 1083, 14220 Prague 4, Czech Republic. Faculty of Natural Science, Charles University, Vinicna 7, 12843 Prague 2, Czech Republic.Institute of Experimental Medicine of the Czech Academy of Sciences, Vídenska 1083, 14220 Prague 4, Czech Republic.Laboratoires Thea (Thea-Pharma), 12 Rue Louis-Bleriot, 63000 Clermont-Ferrand, France.Institute of Experimental Medicine of the Czech Academy of Sciences, Vídenska 1083, 14220 Prague 4, Czech Republic.

Pub Type(s)

Journal Article
Retracted Publication

Language

eng

PubMed ID

31641422

Citation

Cejka, Cestmir, et al. "The Healing of Oxidative Injuries With Trehalose in UVB-Irradiated Rabbit Corneas." Oxidative Medicine and Cellular Longevity, vol. 2019, 2019, p. 1857086.
Cejka C, Kossl J, Hermankova B, et al. The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas. Oxid Med Cell Longev. 2019;2019:1857086.
Cejka, C., Kossl, J., Hermankova, B., Holan, V., Kubinova, S., Olmiere, C., & Cejkova, J. (2019). The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas. Oxidative Medicine and Cellular Longevity, 2019, 1857086. https://doi.org/10.1155/2019/1857086
Cejka C, et al. The Healing of Oxidative Injuries With Trehalose in UVB-Irradiated Rabbit Corneas. Oxid Med Cell Longev. 2019;2019:1857086. PubMed PMID: 31641422.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas. AU - Cejka,Cestmir, AU - Kossl,Jan, AU - Hermankova,Barbora, AU - Holan,Vladimir, AU - Kubinova,Sarka, AU - Olmiere,Céline, AU - Cejkova,Jitka, Y1 - 2019/09/19/ PY - 2019/03/27/received PY - 2019/06/25/revised PY - 2019/08/20/accepted PY - 2019/10/24/entrez PY - 2019/10/24/pubmed PY - 2020/3/27/medline SP - 1857086 EP - 1857086 JF - Oxidative medicine and cellular longevity JO - Oxid Med Cell Longev VL - 2019 N2 - Our previous research revealed that trehalose, a nonreducing disaccharide of glucose and an important stress responsive factor, proved to have anti-inflammatory, antiapoptotic, and particularly antioxidant properties in UVB-irradiated corneas. Trehalose reduced oxidative stress in corneas induced by UVB irradiation, by means of a decrease in the antioxidant/prooxidant imbalance in the corneal epithelium. In this study, we demonstrate that trehalose of 3% or 6% concentration in eye drops directly decreases oxidative stress in UVB-irradiated corneas, by removing the excessive amount of reactive oxygen species (ROS). Trehalose drops applied on corneas during UVB irradiation once daily for four days resulted in a reduction or even absence of the oxidative stress, DNA damage, and peroxynitrite formation (detected by nitrotyrosine residues), seen in buffer-treated corneas. Furthermore, trehalose treatment applied curatively after repeated irradiation for the subsequent fourteen days led to the renewal of corneal transparency and significant suppression or even absence of neovascularization. This was in contrast to buffer-treated irradiated corneas, where the intracorneal inflammation was developed and the untransparent corneas were vascularized. In conclusion, the treatment of UVB-irradiated corneas with trehalose eye drops removed the excessive amount of ROS in the corneal epithelium, leading to the suppression of oxidative stress and favorable corneal healing. The 6% trehalose showed a higher intensive antioxidant effect. SN - 1942-0994 UR - https://www.unboundmedicine.com/medline/citation/31641422/The_Healing_of_Oxidative_Injuries_with_Trehalose_in_UVB_Irradiated_Rabbit_Corneas_ L2 - https://doi.org/10.1155/2019/1857086 DB - PRIME DP - Unbound Medicine ER -