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[Determination of benzimidazoles residue markers in chicken tissue by high performance liquid chromatography].
Se Pu. 2019 Oct 08; 37(10):1112-1117.SP

Abstract

A method was developed for the simultaneous determination of 11 benzimildazoles residue markers in chicken meat, liver and kidney samples by high performance liquid chromatography (HPLC). The chicken tissue sample was extracted with acetonitrile, defatted with n-hexane, and purified with an MCX solid-phase extraction cartridge. Chromatographic separation was performed on an Atlantis T3 column with the gradient elution of methyl alcohol-1% (v/v) acetic acid aqueous solution as the mobile phases at a flow rate of 1.0 mL/min. The detection wavelength was set at 292 nm. Analytes were quantified by the external standard method. The linearities of the calibration curves were well in the range of 25-1000 μg/L, and the limit of quantification was 50 μg/kg. The recoveries at different spiked levels in all the three matrices ranged from 70.91% to 103.21%, and the relative standard deviations (RSDs) were between 1.48% and 10.08% (n=6). The developed method is characterized by an excellent purification effect, sensitivity and accuracy, and can be used for the determination of benzimidazoles residue markers in chicken tissue samples.

Authors+Show Affiliations

Guangdong Inspection and Quarantine Technology Center, Guangzhou 510623, China.Guangdong Inspection and Quarantine Technology Center, Guangzhou 510623, China.Guangdong Inspection and Quarantine Technology Center, Guangzhou 510623, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31642291

Citation

Zou, You, et al. "[Determination of Benzimidazoles Residue Markers in Chicken Tissue By High Performance Liquid Chromatography]." Se Pu = Chinese Journal of Chromatography, vol. 37, no. 10, 2019, pp. 1112-1117.
Zou Y, Shao L, Wu Y. [Determination of benzimidazoles residue markers in chicken tissue by high performance liquid chromatography]. Se Pu. 2019;37(10):1112-1117.
Zou, Y., Shao, L., & Wu, Y. (2019). [Determination of benzimidazoles residue markers in chicken tissue by high performance liquid chromatography]. Se Pu = Chinese Journal of Chromatography, 37(10), 1112-1117. https://doi.org/10.3724/SP.J.1123.2019.03031
Zou Y, Shao L, Wu Y. [Determination of Benzimidazoles Residue Markers in Chicken Tissue By High Performance Liquid Chromatography]. Se Pu. 2019 Oct 8;37(10):1112-1117. PubMed PMID: 31642291.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of benzimidazoles residue markers in chicken tissue by high performance liquid chromatography]. AU - Zou,You, AU - Shao,Linzhi, AU - Wu,Yingxuan, PY - 2019/10/24/entrez PY - 2019/10/24/pubmed PY - 2019/10/28/medline KW - benzimidazoles residue markers KW - chicken tissue KW - high performance liquid chromatography (HPLC) KW - solid phase extraction(SPE) SP - 1112 EP - 1117 JF - Se pu = Chinese journal of chromatography JO - Se Pu VL - 37 IS - 10 N2 - A method was developed for the simultaneous determination of 11 benzimildazoles residue markers in chicken meat, liver and kidney samples by high performance liquid chromatography (HPLC). The chicken tissue sample was extracted with acetonitrile, defatted with n-hexane, and purified with an MCX solid-phase extraction cartridge. Chromatographic separation was performed on an Atlantis T3 column with the gradient elution of methyl alcohol-1% (v/v) acetic acid aqueous solution as the mobile phases at a flow rate of 1.0 mL/min. The detection wavelength was set at 292 nm. Analytes were quantified by the external standard method. The linearities of the calibration curves were well in the range of 25-1000 μg/L, and the limit of quantification was 50 μg/kg. The recoveries at different spiked levels in all the three matrices ranged from 70.91% to 103.21%, and the relative standard deviations (RSDs) were between 1.48% and 10.08% (n=6). The developed method is characterized by an excellent purification effect, sensitivity and accuracy, and can be used for the determination of benzimidazoles residue markers in chicken tissue samples. SN - 1000-8713 UR - https://www.unboundmedicine.com/medline/citation/31642291/[Determination_of_benzimidazoles_residue_markers_in_chicken_tissue_by_high_performance_liquid_chromatography]_ DB - PRIME DP - Unbound Medicine ER -