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Assessment of hematological, hepato-renal, antioxidant, and hormonal responses of Clarias gariepinus exposed to sub-lethal concentrations of oxyfluorfen.
Aquat Toxicol. 2019 Dec; 217:105329.AT

Abstract

Little is known about the effects of oxyfluorfen, a diphenyl ether herbicide, exposure on the African catfish (Clarias gariepinus) health. Consequently, the existing investigation was designed to highlight the impacts of oxyfluorfen exposure on C. gariepinus hematological indices, liver and kidney functions, reproductive hormones, and oxidative status. Furthermore, a consequent 10-day depuration period was adopted to evaluate the recovery of the disturbed indicators to normal values. In the first experiment, the 96-h lethal concentration 50 (LC50) of oxyfluorfen for C. gariepinus was determined using probit analysis. Next, in a second experiment, 180 healthy fish (average initial body weight: 164.23 ± 0.24) were randomly assigned to 4 experimental groups exposed to 0, 1/10, 1/8, or 1/5 96-h LC50 of oxyfluorfen. The hematological profile, hepatic enzymes, kidney damage byproducts, reproductive hormones, oxidative stress, and lipid peroxidation indicators together with acetylcholinesterase (AChE) content were assessed. A histopathological examination of the hepatic, renal, brain, and testicular tissues was accomplished. Moreover, the expression of the oxidative stress-related gene was carried out. The results showed that 96-h LC50 of oxyfluorfen for C. gariepinus was 11.698 mg/L. Exposure to sublethal levels of oxyfluorfen induced macrocytic hypochromic anemia, leukopenia, lymphopenia, monocytopenia, and eosinopenia. Also, a concentration-dependent increase in alanine transaminase, alkaline phosphatase, aspartate transaminase, urea, creatinine, catalase, and malondialdehyde was detected following oxyfluorfen exposure together with upregulation of catalase gene. But, significant concentration-dependent reductions in AChE, glutathione transferase, reduced to oxidized glutathione ratio, estradiol, and testosterone activities were recorded. These biochemical alterations were accompanied by pathological perturbations in hepatic, renal, brain, and testicular tissues. Following 10 days of recovery, only the hematological impairments were abolished. Conclusively, the herbicides oxyfluorfen could induce multiple negative impacts on C. gariepinus with oxidative stress as a probable underlying mechanism. Additionally, a recovery period of 10 days was not enough to restore these impairments.

Authors+Show Affiliations

Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt.Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt.Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt.Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt. Electronic address: yasmina_forensic@hotmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31648108

Citation

Abd El-Rahman, Ghada I., et al. "Assessment of Hematological, Hepato-renal, Antioxidant, and Hormonal Responses of Clarias Gariepinus Exposed to Sub-lethal Concentrations of Oxyfluorfen." Aquatic Toxicology (Amsterdam, Netherlands), vol. 217, 2019, p. 105329.
Abd El-Rahman GI, Ahmed SAA, Khalil AA, et al. Assessment of hematological, hepato-renal, antioxidant, and hormonal responses of Clarias gariepinus exposed to sub-lethal concentrations of oxyfluorfen. Aquat Toxicol. 2019;217:105329.
Abd El-Rahman, G. I., Ahmed, S. A. A., Khalil, A. A., & Abd-Elhakim, Y. M. (2019). Assessment of hematological, hepato-renal, antioxidant, and hormonal responses of Clarias gariepinus exposed to sub-lethal concentrations of oxyfluorfen. Aquatic Toxicology (Amsterdam, Netherlands), 217, 105329. https://doi.org/10.1016/j.aquatox.2019.105329
Abd El-Rahman GI, et al. Assessment of Hematological, Hepato-renal, Antioxidant, and Hormonal Responses of Clarias Gariepinus Exposed to Sub-lethal Concentrations of Oxyfluorfen. Aquat Toxicol. 2019;217:105329. PubMed PMID: 31648108.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of hematological, hepato-renal, antioxidant, and hormonal responses of Clarias gariepinus exposed to sub-lethal concentrations of oxyfluorfen. AU - Abd El-Rahman,Ghada I, AU - Ahmed,Shaimaa A A, AU - Khalil,Alshimaa A, AU - Abd-Elhakim,Yasmina M, Y1 - 2019/10/12/ PY - 2019/08/26/received PY - 2019/10/04/revised PY - 2019/10/05/accepted PY - 2019/10/28/pubmed PY - 2019/10/28/medline PY - 2019/10/25/entrez KW - Acetylchloinestrase KW - Clarias gariepinus KW - Hepatorenal damage KW - LC(50) KW - Oxidative stress KW - Oxyfluorfen SP - 105329 EP - 105329 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat. Toxicol. VL - 217 N2 - Little is known about the effects of oxyfluorfen, a diphenyl ether herbicide, exposure on the African catfish (Clarias gariepinus) health. Consequently, the existing investigation was designed to highlight the impacts of oxyfluorfen exposure on C. gariepinus hematological indices, liver and kidney functions, reproductive hormones, and oxidative status. Furthermore, a consequent 10-day depuration period was adopted to evaluate the recovery of the disturbed indicators to normal values. In the first experiment, the 96-h lethal concentration 50 (LC50) of oxyfluorfen for C. gariepinus was determined using probit analysis. Next, in a second experiment, 180 healthy fish (average initial body weight: 164.23 ± 0.24) were randomly assigned to 4 experimental groups exposed to 0, 1/10, 1/8, or 1/5 96-h LC50 of oxyfluorfen. The hematological profile, hepatic enzymes, kidney damage byproducts, reproductive hormones, oxidative stress, and lipid peroxidation indicators together with acetylcholinesterase (AChE) content were assessed. A histopathological examination of the hepatic, renal, brain, and testicular tissues was accomplished. Moreover, the expression of the oxidative stress-related gene was carried out. The results showed that 96-h LC50 of oxyfluorfen for C. gariepinus was 11.698 mg/L. Exposure to sublethal levels of oxyfluorfen induced macrocytic hypochromic anemia, leukopenia, lymphopenia, monocytopenia, and eosinopenia. Also, a concentration-dependent increase in alanine transaminase, alkaline phosphatase, aspartate transaminase, urea, creatinine, catalase, and malondialdehyde was detected following oxyfluorfen exposure together with upregulation of catalase gene. But, significant concentration-dependent reductions in AChE, glutathione transferase, reduced to oxidized glutathione ratio, estradiol, and testosterone activities were recorded. These biochemical alterations were accompanied by pathological perturbations in hepatic, renal, brain, and testicular tissues. Following 10 days of recovery, only the hematological impairments were abolished. Conclusively, the herbicides oxyfluorfen could induce multiple negative impacts on C. gariepinus with oxidative stress as a probable underlying mechanism. Additionally, a recovery period of 10 days was not enough to restore these impairments. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/31648108/Assessment_of_hematological_hepato_renal_antioxidant_and_hormonal_responses_of_Clarias_gariepinus_exposed_to_sub_lethal_concentrations_of_oxyfluorfen_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(19)30681-2 DB - PRIME DP - Unbound Medicine ER -