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Genetic polymorphism of drug metabolism enzymes (GSTM1, GSTT1 and GSTP1) in the healthy Malian population.
Mol Biol Rep. 2020 Jan; 47(1):393-400.MB

Abstract

Glutathione S-transferase genes, known to be highly polymorphic, are implicated in the process of phase II metabolism of many substrates, including xenobiotics, anticancer and anti-infective drugs. The detoxification activity is linked to individual genetic makeup. Therefore, the identification of alleles and genotypes in these genes within a population may help to better design genetic susceptibility and pharmacogenetic studies. We performed the present study to establish the frequencies of the GSTM1, GSTT1, and GSTP1 c. 313A > G (rs1695) polymorphisms in 206 individuals of the Malian healthy population. GSTM1 and GSTT1 were genotyped by using multiplex polymerase chain reaction, whereas genotypes of GSTP1 were identified by polymerase chain reaction followed by restriction fragment length polymorphism. The frequencies of GSTM1-null and GSTT1-null genotypes were respectively 24.3 and 41.3%. The observed genotype frequencies for GSTP1 were 25.73% homozygous wild-type AA, 49.03% heterozygous AG and 25.24% homozygous mutant GG. The frequency of GSTP1-A allele was 50.24% versus 49.76% for the GSTP1-G allele. The distribution of these three genes was homogeneous between men and women (p > 0.05). We found no statistical association between the presence of a particular profile of GSTM1 or GSTT1 with the genotypes of GSTP1 (p > 0.05). Nevertheless, we noticed that the majority of the individuals harboring the GSTM1-present or the GSTT1-present harbor also the GSTP1-AG genotype. In addition, the triple genotype GSTM1-present/GSTT1-present/AG was the most frequent with 25.2%. Our findings will facilitate future studies regarding genetic associations of multifactorial diseases and pharmacogenetic, thus opening the way to personalized medicine in our population.

Authors+Show Affiliations

Faculty of Medicine and Odontostomatology, University of Sciences Techniques and Technologies of Bamako, Point G, BP. 1805, Bamako, Mali. kassoy2@yahoo.fr.Faculty of Medicine and Odontostomatology, University of Sciences Techniques and Technologies of Bamako, Point G, BP. 1805, Bamako, Mali.Faculty of Medicine and Odontostomatology, University of Sciences Techniques and Technologies of Bamako, Point G, BP. 1805, Bamako, Mali.Faculty of Medicine and Odontostomatology, University of Sciences Techniques and Technologies of Bamako, Point G, BP. 1805, Bamako, Mali.Faculty of Medicine and Odontostomatology, University of Sciences Techniques and Technologies of Bamako, Point G, BP. 1805, Bamako, Mali.Center of Listening, Care, Animation and Counseling, Bamako, Mali.Genetics and Molecular Pathology Laboratory, Medical School of Casablanca, University Hassan II, Casablanca, Morocco.Genetics and Molecular Pathology Laboratory, Medical School of Casablanca, University Hassan II, Casablanca, Morocco.Faculty of Medicine and Odontostomatology, University of Sciences Techniques and Technologies of Bamako, Point G, BP. 1805, Bamako, Mali.Faculty of Medicine and Odontostomatology, University of Sciences Techniques and Technologies of Bamako, Point G, BP. 1805, Bamako, Mali.Faculty of Medicine and Odontostomatology, University of Sciences Techniques and Technologies of Bamako, Point G, BP. 1805, Bamako, Mali.Faculty of Medicine and Odontostomatology, University of Sciences Techniques and Technologies of Bamako, Point G, BP. 1805, Bamako, Mali.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31650384

Citation

Kassogue, Yaya, et al. "Genetic Polymorphism of Drug Metabolism Enzymes (GSTM1, GSTT1 and GSTP1) in the Healthy Malian Population." Molecular Biology Reports, vol. 47, no. 1, 2020, pp. 393-400.
Kassogue Y, Diakite B, Kassogue O, et al. Genetic polymorphism of drug metabolism enzymes (GSTM1, GSTT1 and GSTP1) in the healthy Malian population. Mol Biol Rep. 2020;47(1):393-400.
Kassogue, Y., Diakite, B., Kassogue, O., Konate, I., Tamboura, K., Diarra, Z., Dehbi, H., Nadifi, S., Traore, C. B., Dao, S., Doumbia, S., & Dolo, G. (2020). Genetic polymorphism of drug metabolism enzymes (GSTM1, GSTT1 and GSTP1) in the healthy Malian population. Molecular Biology Reports, 47(1), 393-400. https://doi.org/10.1007/s11033-019-05143-5
Kassogue Y, et al. Genetic Polymorphism of Drug Metabolism Enzymes (GSTM1, GSTT1 and GSTP1) in the Healthy Malian Population. Mol Biol Rep. 2020;47(1):393-400. PubMed PMID: 31650384.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic polymorphism of drug metabolism enzymes (GSTM1, GSTT1 and GSTP1) in the healthy Malian population. AU - Kassogue,Yaya, AU - Diakite,Brehima, AU - Kassogue,Oumar, AU - Konate,Issa, AU - Tamboura,Kadidiatou, AU - Diarra,Zoumana, AU - Dehbi,Hind, AU - Nadifi,Sellama, AU - Traore,Cheick Bougadari, AU - Dao,Sounkalo, AU - Doumbia,Seydou, AU - Dolo,Guimogo, Y1 - 2019/10/24/ PY - 2019/08/29/received PY - 2019/10/15/accepted PY - 2019/10/28/pubmed PY - 2020/6/2/medline PY - 2019/10/26/entrez KW - GSTM1 KW - GSTP1 c. 313A > G KW - GSTT1 KW - Genotype KW - Mali SP - 393 EP - 400 JF - Molecular biology reports JO - Mol Biol Rep VL - 47 IS - 1 N2 - Glutathione S-transferase genes, known to be highly polymorphic, are implicated in the process of phase II metabolism of many substrates, including xenobiotics, anticancer and anti-infective drugs. The detoxification activity is linked to individual genetic makeup. Therefore, the identification of alleles and genotypes in these genes within a population may help to better design genetic susceptibility and pharmacogenetic studies. We performed the present study to establish the frequencies of the GSTM1, GSTT1, and GSTP1 c. 313A > G (rs1695) polymorphisms in 206 individuals of the Malian healthy population. GSTM1 and GSTT1 were genotyped by using multiplex polymerase chain reaction, whereas genotypes of GSTP1 were identified by polymerase chain reaction followed by restriction fragment length polymorphism. The frequencies of GSTM1-null and GSTT1-null genotypes were respectively 24.3 and 41.3%. The observed genotype frequencies for GSTP1 were 25.73% homozygous wild-type AA, 49.03% heterozygous AG and 25.24% homozygous mutant GG. The frequency of GSTP1-A allele was 50.24% versus 49.76% for the GSTP1-G allele. The distribution of these three genes was homogeneous between men and women (p > 0.05). We found no statistical association between the presence of a particular profile of GSTM1 or GSTT1 with the genotypes of GSTP1 (p > 0.05). Nevertheless, we noticed that the majority of the individuals harboring the GSTM1-present or the GSTT1-present harbor also the GSTP1-AG genotype. In addition, the triple genotype GSTM1-present/GSTT1-present/AG was the most frequent with 25.2%. Our findings will facilitate future studies regarding genetic associations of multifactorial diseases and pharmacogenetic, thus opening the way to personalized medicine in our population. SN - 1573-4978 UR - https://www.unboundmedicine.com/medline/citation/31650384/Genetic_polymorphism_of_drug_metabolism_enzymes__GSTM1_GSTT1_and_GSTP1__in_the_healthy_Malian_population_ L2 - https://doi.org/10.1007/s11033-019-05143-5 DB - PRIME DP - Unbound Medicine ER -