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Construction of a Competitive Endogenous RNA Network in Uterine Corpus Endometrial Carcinoma.
Med Sci Monit. 2019 Oct 25; 25:7998-8010.MS

Abstract

BACKGROUND

Long non-coding RNAs (lncRNAs) affect post-transcriptional regulation by interfering with microRNAs (miRNAs), and by acting as competitive endogenous RNAs (ceRNAs). The roles and mechanisms of lncRNAs as ceRNAs in the progression and prognosis of uterine corpus endometrial carcinoma are not well understood. MATERIAL AND

METHODS

We analyzed high-throughput transcriptome data downloaded from The Cancer Genome Atlas database for 548 patients with uterine corpus endometrial carcinoma, and the we constructed a ceRNA network. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses of differentially expressed messenger RNAs (DE-mRNAs) were performed using R software. Kaplan-Meier survival curves were generated for all RNAs in the ceRNA network.

RESULTS

We identified 2612 messenger RNAs (mRNAs), 1111 lncRNAs, and 187 miRNAs that were differentially expressed in uterine corpus endometrial carcinoma. We then identified mutual regulatory relationships between lncRNA-miRNA pairs and miRNA-mRNA pairs. A ceRNA regulatory network for uterine corpus endometrial carcinoma was successfully constructed, and consisted of 87 lncRNAs, 74 mRNAs, and 20 miRNAs. Nine lncRNAs, 3 miRNAs, and 22 mRNAs were associated with prognosis of uterine corpus endometrial carcinoma. We also analyzed the linear relationships between the expression of the 9 DE-lncRNAs and 22 DE-mRNAs with prognostic value.

CONCLUSIONS

Our study showed that the lncRNAs C2orf48 and LINC00261 might be key regulators of uterine corpus endometrial carcinoma and might serve as prognostic indicators. Our study contributes to the understanding of the molecular mechanisms of uterine corpus endometrial carcinoma, and it identifies lncRNAs that might serve as prognostic markers and therapeutic targets.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Akesu Hospital of Traditional Chinese Medicine, Akesu, Xinjiang, China (mainland).Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).Department of Obstetrics and Gynecology, Akesu Hospital of Traditional Chinese Medicine, Akesu, Xinjiang, China (mainland).Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31650984

Citation

Ouyang, Dong, et al. "Construction of a Competitive Endogenous RNA Network in Uterine Corpus Endometrial Carcinoma." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 25, 2019, pp. 7998-8010.
Ouyang D, Li R, Li Y, et al. Construction of a Competitive Endogenous RNA Network in Uterine Corpus Endometrial Carcinoma. Med Sci Monit. 2019;25:7998-8010.
Ouyang, D., Li, R., Li, Y., & Zhu, X. (2019). Construction of a Competitive Endogenous RNA Network in Uterine Corpus Endometrial Carcinoma. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 7998-8010. https://doi.org/10.12659/MSM.915798
Ouyang D, et al. Construction of a Competitive Endogenous RNA Network in Uterine Corpus Endometrial Carcinoma. Med Sci Monit. 2019 Oct 25;25:7998-8010. PubMed PMID: 31650984.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of a Competitive Endogenous RNA Network in Uterine Corpus Endometrial Carcinoma. AU - Ouyang,Dong, AU - Li,Ruyi, AU - Li,Yaxian, AU - Zhu,Xueqiong, Y1 - 2019/10/25/ PY - 2019/10/26/entrez PY - 2019/10/28/pubmed PY - 2020/3/3/medline SP - 7998 EP - 8010 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med. Sci. Monit. VL - 25 N2 - BACKGROUND Long non-coding RNAs (lncRNAs) affect post-transcriptional regulation by interfering with microRNAs (miRNAs), and by acting as competitive endogenous RNAs (ceRNAs). The roles and mechanisms of lncRNAs as ceRNAs in the progression and prognosis of uterine corpus endometrial carcinoma are not well understood. MATERIAL AND METHODS We analyzed high-throughput transcriptome data downloaded from The Cancer Genome Atlas database for 548 patients with uterine corpus endometrial carcinoma, and the we constructed a ceRNA network. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses of differentially expressed messenger RNAs (DE-mRNAs) were performed using R software. Kaplan-Meier survival curves were generated for all RNAs in the ceRNA network. RESULTS We identified 2612 messenger RNAs (mRNAs), 1111 lncRNAs, and 187 miRNAs that were differentially expressed in uterine corpus endometrial carcinoma. We then identified mutual regulatory relationships between lncRNA-miRNA pairs and miRNA-mRNA pairs. A ceRNA regulatory network for uterine corpus endometrial carcinoma was successfully constructed, and consisted of 87 lncRNAs, 74 mRNAs, and 20 miRNAs. Nine lncRNAs, 3 miRNAs, and 22 mRNAs were associated with prognosis of uterine corpus endometrial carcinoma. We also analyzed the linear relationships between the expression of the 9 DE-lncRNAs and 22 DE-mRNAs with prognostic value. CONCLUSIONS Our study showed that the lncRNAs C2orf48 and LINC00261 might be key regulators of uterine corpus endometrial carcinoma and might serve as prognostic indicators. Our study contributes to the understanding of the molecular mechanisms of uterine corpus endometrial carcinoma, and it identifies lncRNAs that might serve as prognostic markers and therapeutic targets. SN - 1643-3750 UR - https://www.unboundmedicine.com/medline/citation/31650984/Construction_of_a_Competitive_Endogenous_RNA_Network_in_Uterine_Corpus_Endometrial_Carcinoma_ L2 - https://www.medscimonit.com/download/index/idArt/915798 DB - PRIME DP - Unbound Medicine ER -