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Roles of glycoprotein glycosylation in the pathogenesis and effectiveness evaluation of the sodium hyaluronate treatment of an endemic osteoarthritis Kashin-Beck disease.
Turk J Med Sci. 2019 Oct 27 [Online ahead of print]TJ

Abstract

BACKGROUND/AIM

We aimed to explore the roles of glycoproteins glycosylation in the pathogenesis of Kashin-Beck disease (KBD) and evaluated the effectiveness of sodium hyaluronate treatment.

MATERIALS AND METHODS

Blood and saliva were collected from KBD patients before and after the injection of sodium hyaluronate. Normal healthy subjects were included as controls. Saliva and serum lectin microarrays and saliva and serum microarray verifications were used to screen and confirm the differences in lectin levels among the three groups.

RESULTS

In saliva lectin microarray, bindings to Sophora Japonica Agglutinin (SJA), Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL-I), Griffonia (Bandeiraea) Simplicifolia Lectin I (EEL), Maackia Amurensis Lectin II (MAL-II), Sambucus Nigra Lectin (SNA), Hippeastrum Hybrid Lectin (HHL) and Aleuria Aurantia Lectin (AAL) were higher in the untreated KBD patients than in the control group. Increased levels for HHL, MAL-II and GSL-I in the untreated KBD patients discriminated them in particular from the treated ones. Jacalin was lower in the untreated KBD patients compared to the treated KBD and the normal groups. In serum lectin microarray, HHL and Peanut Agglutinin (PNA) were increased in the untreated KBD group in comparison to the control one. AAL, Phaseolus vulgaris Agglutinin(E+L) (PHA-E+L) and PsophocarpusTetragonolobus Lectin I (PTL-I) were lower in the untreated KBD patients compared to the treated KBD and the normal groups. Hyaluronate treatment appeared to normalize SNA, AAL and MAL-II levels in saliva, and HHL, PNA, AAL, PTL-I and PHA-E+L levels in serum. Saliva reversed microarray verification confirmed significant differences between groups in SNA and Jacalin, in particular, GSL-I levels, while serum reversed microarray verification indicated that HHL, PNA and AAL levels returned to normal level after the hyaluronate treatment. Lectin blot confirmed significant differences in HHL, AAL and Jaclin in saliva, and HHL, PNA, PHA-E+L and AAL in serum.

CONCLUSION

HHL in saliva and serum may be valuable diagnostic biomarker of KBD, and it may be used to follow-up of the hyaluronate treatment.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31655502

Citation

Wang, Sen, et al. "Roles of Glycoprotein Glycosylation in the Pathogenesis and Effectiveness Evaluation of the Sodium Hyaluronate Treatment of an Endemic Osteoarthritis Kashin-Beck Disease." Turkish Journal of Medical Sciences, 2019.
Wang S, Gao Z, Liu H, et al. Roles of glycoprotein glycosylation in the pathogenesis and effectiveness evaluation of the sodium hyaluronate treatment of an endemic osteoarthritis Kashin-Beck disease. Turk J Med Sci. 2019.
Wang, S., Gao, Z., Liu, H., Meng, P., Wu, C., Lammi, M., & Guo, X. (2019). Roles of glycoprotein glycosylation in the pathogenesis and effectiveness evaluation of the sodium hyaluronate treatment of an endemic osteoarthritis Kashin-Beck disease. Turkish Journal of Medical Sciences. https://doi.org/10.3906/sag-1903-137
Wang S, et al. Roles of Glycoprotein Glycosylation in the Pathogenesis and Effectiveness Evaluation of the Sodium Hyaluronate Treatment of an Endemic Osteoarthritis Kashin-Beck Disease. Turk J Med Sci. 2019 Oct 27; PubMed PMID: 31655502.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Roles of glycoprotein glycosylation in the pathogenesis and effectiveness evaluation of the sodium hyaluronate treatment of an endemic osteoarthritis Kashin-Beck disease. AU - Wang,Sen, AU - Gao,Zongqiang, AU - Liu,Huan, AU - Meng,Peilin, AU - Wu,Cuiyan, AU - Lammi,Mikko, AU - Guo,Xiong, Y1 - 2019/10/27/ PY - 2019/03/18/received PY - 2019/07/21/accepted PY - 2019/10/28/entrez PY - 2019/10/28/pubmed PY - 2019/10/28/medline KW - Kashin-Beck disease KW - carbohydrate chain KW - lectin microarray KW - saliva KW - serum JF - Turkish journal of medical sciences JO - Turk J Med Sci N2 - BACKGROUND/AIM: We aimed to explore the roles of glycoproteins glycosylation in the pathogenesis of Kashin-Beck disease (KBD) and evaluated the effectiveness of sodium hyaluronate treatment. MATERIALS AND METHODS: Blood and saliva were collected from KBD patients before and after the injection of sodium hyaluronate. Normal healthy subjects were included as controls. Saliva and serum lectin microarrays and saliva and serum microarray verifications were used to screen and confirm the differences in lectin levels among the three groups. RESULTS: In saliva lectin microarray, bindings to Sophora Japonica Agglutinin (SJA), Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL-I), Griffonia (Bandeiraea) Simplicifolia Lectin I (EEL), Maackia Amurensis Lectin II (MAL-II), Sambucus Nigra Lectin (SNA), Hippeastrum Hybrid Lectin (HHL) and Aleuria Aurantia Lectin (AAL) were higher in the untreated KBD patients than in the control group. Increased levels for HHL, MAL-II and GSL-I in the untreated KBD patients discriminated them in particular from the treated ones. Jacalin was lower in the untreated KBD patients compared to the treated KBD and the normal groups. In serum lectin microarray, HHL and Peanut Agglutinin (PNA) were increased in the untreated KBD group in comparison to the control one. AAL, Phaseolus vulgaris Agglutinin(E+L) (PHA-E+L) and PsophocarpusTetragonolobus Lectin I (PTL-I) were lower in the untreated KBD patients compared to the treated KBD and the normal groups. Hyaluronate treatment appeared to normalize SNA, AAL and MAL-II levels in saliva, and HHL, PNA, AAL, PTL-I and PHA-E+L levels in serum. Saliva reversed microarray verification confirmed significant differences between groups in SNA and Jacalin, in particular, GSL-I levels, while serum reversed microarray verification indicated that HHL, PNA and AAL levels returned to normal level after the hyaluronate treatment. Lectin blot confirmed significant differences in HHL, AAL and Jaclin in saliva, and HHL, PNA, PHA-E+L and AAL in serum. CONCLUSION: HHL in saliva and serum may be valuable diagnostic biomarker of KBD, and it may be used to follow-up of the hyaluronate treatment. SN - 1303-6165 UR - https://www.unboundmedicine.com/medline/citation/31655502/Roles_of_glycoprotein_glycosylation_in_the_pathogenesis_and_effectiveness_evaluation_of_the_sodium_hyaluronate_treatment_of_an_endemic_osteoarthritis_Kashin-Beck_disease L2 - https://doi.org/10.3906/sag-1903-137 DB - PRIME DP - Unbound Medicine ER -
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