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Evaluation of sperm DNA fragmentation and chromatin structure in infertile men with immotile short-tail sperm defect.
Andrologia. 2020 Feb; 52(1):e13445.A

Abstract

Teratozoospermia is characterised by the presence of spermatozoa with abnormal morphology. One of the morphological disorders that lead to male infertility is immotile short-tail sperm (ISTS) defect. In this study, we evaluated the levels of chromatin packing and DNA fragmentation in patients with immotile short-tail sperm defect. Semen samples were obtained from 31 infertile men with ISTS as case group and 31 normozoospermic men as a control group. Protamine status was evaluated using chromomycin A3 (CMA3) staining and sperm DNA fragmentation assessed by sperm chromatin structure assay (SCSA) and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick-end labelling (TUNEL). The percentage of positive CMA3 spermatozoa was significantly higher in patients' samples (22.6 ± 6.9) compared with controls (16.3 ± 4.2) (p < .05) and also mean (±SD) of sperm DNA fragmentation was significantly higher in patients compared with controls, as measured by TUNEL assay (10.45 ± 4.60 vs. 7.03 ± 2.86, p < .05) and SCSA (24.80 ± 13.1 vs. 15.2 ± 7.2, p < .05). According to our study, sperm DNA fragmentation and chromatin packing abnormality are significantly higher in the ISTS samples compared with normal samples. A possible explanation for this relationship is that sperm chromatin condensation and sperm flagellum formation occur during the same phase of spermatogenesis.

Authors+Show Affiliations

Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31657071

Citation

Atshan, Marzieh, et al. "Evaluation of Sperm DNA Fragmentation and Chromatin Structure in Infertile Men With Immotile Short-tail Sperm Defect." Andrologia, vol. 52, no. 1, 2020, pp. e13445.
Atshan M, Kakavand K, Hosseini SH, et al. Evaluation of sperm DNA fragmentation and chromatin structure in infertile men with immotile short-tail sperm defect. Andrologia. 2020;52(1):e13445.
Atshan, M., Kakavand, K., Hosseini, S. H., Sadighi Gilani, M. A., Mohseni Meybodi, A., & Sabbaghian, M. (2020). Evaluation of sperm DNA fragmentation and chromatin structure in infertile men with immotile short-tail sperm defect. Andrologia, 52(1), e13445. https://doi.org/10.1111/and.13445
Atshan M, et al. Evaluation of Sperm DNA Fragmentation and Chromatin Structure in Infertile Men With Immotile Short-tail Sperm Defect. Andrologia. 2020;52(1):e13445. PubMed PMID: 31657071.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of sperm DNA fragmentation and chromatin structure in infertile men with immotile short-tail sperm defect. AU - Atshan,Marzieh, AU - Kakavand,Kianoosh, AU - Hosseini,Seyedeh Hanieh, AU - Sadighi Gilani,Mohammad Ali, AU - Mohseni Meybodi,Anahita, AU - Sabbaghian,Marjan, Y1 - 2019/10/28/ PY - 2017/12/04/received PY - 2019/08/26/revised PY - 2019/09/09/accepted PY - 2019/10/28/pubmed PY - 2020/6/9/medline PY - 2019/10/29/entrez KW - CMA3 KW - DNA integrity KW - ISTS KW - SCSA KW - TUNEL SP - e13445 EP - e13445 JF - Andrologia JO - Andrologia VL - 52 IS - 1 N2 - Teratozoospermia is characterised by the presence of spermatozoa with abnormal morphology. One of the morphological disorders that lead to male infertility is immotile short-tail sperm (ISTS) defect. In this study, we evaluated the levels of chromatin packing and DNA fragmentation in patients with immotile short-tail sperm defect. Semen samples were obtained from 31 infertile men with ISTS as case group and 31 normozoospermic men as a control group. Protamine status was evaluated using chromomycin A3 (CMA3) staining and sperm DNA fragmentation assessed by sperm chromatin structure assay (SCSA) and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick-end labelling (TUNEL). The percentage of positive CMA3 spermatozoa was significantly higher in patients' samples (22.6 ± 6.9) compared with controls (16.3 ± 4.2) (p < .05) and also mean (±SD) of sperm DNA fragmentation was significantly higher in patients compared with controls, as measured by TUNEL assay (10.45 ± 4.60 vs. 7.03 ± 2.86, p < .05) and SCSA (24.80 ± 13.1 vs. 15.2 ± 7.2, p < .05). According to our study, sperm DNA fragmentation and chromatin packing abnormality are significantly higher in the ISTS samples compared with normal samples. A possible explanation for this relationship is that sperm chromatin condensation and sperm flagellum formation occur during the same phase of spermatogenesis. SN - 1439-0272 UR - https://www.unboundmedicine.com/medline/citation/31657071/Evaluation_of_sperm_DNA_fragmentation_and_chromatin_structure_in_infertile_men_with_immotile_short_tail_sperm_defect_ L2 - https://doi.org/10.1111/and.13445 DB - PRIME DP - Unbound Medicine ER -