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[Electroacupuncture alleviated incisional neck pain possibly by suppressing TNF-α expression and increasing IL-4/IL-4 receptor signaling in cervical dorsal part of spinal cord in incisio-nal neck pain rats].
Zhen Ci Yan Jiu. 2019 Oct 25; 44(10):703-8.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on incisional pain and expression of tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10) and interleukin-4 (IL-4) of cervical dorsal part of spinal cord in rats with incisional neck pain, so as to explore its analgesic mechanisms.

METHODS

Eighty-four male SD rats were randomly divided into normal control, model, EA-Futu(LI18) and EA-Zusanli(ST36)-Yanglingquan(GB34, EA-ST36-GB34) groups (n=21 in each group). The incisional neck pain model was established by making a longitudinal incision along the bilateral cervical thyroid regions and repeated mechanical separation stimulation. For rats of the EA groups, EA (2 Hz/100 Hz, 1 mA) was applied to bilateral LI18 or ST36-GB34 for 30 min/ time during the surgery, and 20 and 44 h after surgery, respectively. The thermal pain threshold (TPT) of the incisional region was detected. The immunoactivity of TNF-α and IL-10 of the dorsal portion of the cervical spinal cord (C2-C5) was detected by immunofluorescence, and the expression of TNF-α, IL-10, IL-4 and IL-4 receptor (IL-4R) mRNAs was determined by quantitative real-time PCR.

RESULTS

Compared with the normal group, the TPT of the incisional area was significantly decreased at 4, 24 and 48 h after neck-incision (P<0.05), the levels of TNF-α mRNA, IL-10 mRNA and TNF-α IL-10 immunoactivity at 24 h were remarkably increased (P<0.05), and the expression of IL-4R mRNA was considerably decreased at 24 h in the model group (P<0.05). Following EA intervention, the TPT, and expression levels of IL-4 mRNA and IL-4R mRNA were significantly increased at 24 h after surgery in the EA-LI18 group relevant to the model group (P<0.05), while the expression level of TNF-α(coexpressed with microgliacytes) in the EA-LI18 group, and TNF-α mRNA expression at 24 h in both EA-LI18 and EA-ST36-GB34 groups, as well as the expression of IL-10 and IL-10 mRNA at 24 h in both EA-LI18 and EA-ST36-GB34 groups were significantly decreased (P<0.05). The effect of EA LI18 was significantly superior to that of EA ST36-GB34 in up-regulating TPT and expression of IL-4 mRNA and IL-4R mRNA at 24 h (P<0.05).

CONCLUSION

EA of LI18 has an analgesic effect in incisional neck pain rats, which may be related to its effect in down-regulating the expression of TNF-α, IL-10 and promoting IL-4 /IL-4R signaling in dorsal portions of the cervical spinal cord. The analgesic effect of EA LI18 is better than that of EA ST36-GB34.

Authors+Show Affiliations

Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31657158

Citation

Wang, Jun-Ying, et al. "[Electroacupuncture Alleviated Incisional Neck Pain Possibly By Suppressing TNF-α Expression and Increasing IL-4/IL-4 Receptor Signaling in Cervical Dorsal Part of Spinal Cord in Incisio-nal Neck Pain Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 44, no. 10, 2019, pp. 703-8.
Wang JY, Gao YH, Qiao LN, et al. [Electroacupuncture alleviated incisional neck pain possibly by suppressing TNF-α expression and increasing IL-4/IL-4 receptor signaling in cervical dorsal part of spinal cord in incisio-nal neck pain rats]. Zhen Ci Yan Jiu. 2019;44(10):703-8.
Wang, J. Y., Gao, Y. H., Qiao, L. N., Zhang, J. L., Rong, P. J., & Liu, J. L. (2019). [Electroacupuncture alleviated incisional neck pain possibly by suppressing TNF-α expression and increasing IL-4/IL-4 receptor signaling in cervical dorsal part of spinal cord in incisio-nal neck pain rats]. Zhen Ci Yan Jiu = Acupuncture Research, 44(10), 703-8. https://doi.org/10.13702/j.1000-0607.190355
Wang JY, et al. [Electroacupuncture Alleviated Incisional Neck Pain Possibly By Suppressing TNF-α Expression and Increasing IL-4/IL-4 Receptor Signaling in Cervical Dorsal Part of Spinal Cord in Incisio-nal Neck Pain Rats]. Zhen Ci Yan Jiu. 2019 Oct 25;44(10):703-8. PubMed PMID: 31657158.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Electroacupuncture alleviated incisional neck pain possibly by suppressing TNF-α expression and increasing IL-4/IL-4 receptor signaling in cervical dorsal part of spinal cord in incisio-nal neck pain rats]. AU - Wang,Jun-Ying, AU - Gao,Yong-Hui, AU - Qiao,Li-Na, AU - Zhang,Jin-Ling, AU - Rong,Pei-Jing, AU - Liu,Jun-Ling, PY - 2019/10/29/entrez PY - 2019/10/28/pubmed PY - 2019/11/15/medline KW - Dorsal horns of cervical spinal cord KW - Electroacupuncture KW - Incisional neck pain KW - Inflammatory cytokines SP - 703 EP - 8 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 44 IS - 10 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) on incisional pain and expression of tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10) and interleukin-4 (IL-4) of cervical dorsal part of spinal cord in rats with incisional neck pain, so as to explore its analgesic mechanisms. METHODS: Eighty-four male SD rats were randomly divided into normal control, model, EA-Futu(LI18) and EA-Zusanli(ST36)-Yanglingquan(GB34, EA-ST36-GB34) groups (n=21 in each group). The incisional neck pain model was established by making a longitudinal incision along the bilateral cervical thyroid regions and repeated mechanical separation stimulation. For rats of the EA groups, EA (2 Hz/100 Hz, 1 mA) was applied to bilateral LI18 or ST36-GB34 for 30 min/ time during the surgery, and 20 and 44 h after surgery, respectively. The thermal pain threshold (TPT) of the incisional region was detected. The immunoactivity of TNF-α and IL-10 of the dorsal portion of the cervical spinal cord (C2-C5) was detected by immunofluorescence, and the expression of TNF-α, IL-10, IL-4 and IL-4 receptor (IL-4R) mRNAs was determined by quantitative real-time PCR. RESULTS: Compared with the normal group, the TPT of the incisional area was significantly decreased at 4, 24 and 48 h after neck-incision (P<0.05), the levels of TNF-α mRNA, IL-10 mRNA and TNF-α IL-10 immunoactivity at 24 h were remarkably increased (P<0.05), and the expression of IL-4R mRNA was considerably decreased at 24 h in the model group (P<0.05). Following EA intervention, the TPT, and expression levels of IL-4 mRNA and IL-4R mRNA were significantly increased at 24 h after surgery in the EA-LI18 group relevant to the model group (P<0.05), while the expression level of TNF-α(coexpressed with microgliacytes) in the EA-LI18 group, and TNF-α mRNA expression at 24 h in both EA-LI18 and EA-ST36-GB34 groups, as well as the expression of IL-10 and IL-10 mRNA at 24 h in both EA-LI18 and EA-ST36-GB34 groups were significantly decreased (P<0.05). The effect of EA LI18 was significantly superior to that of EA ST36-GB34 in up-regulating TPT and expression of IL-4 mRNA and IL-4R mRNA at 24 h (P<0.05). CONCLUSION: EA of LI18 has an analgesic effect in incisional neck pain rats, which may be related to its effect in down-regulating the expression of TNF-α, IL-10 and promoting IL-4 /IL-4R signaling in dorsal portions of the cervical spinal cord. The analgesic effect of EA LI18 is better than that of EA ST36-GB34. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/31657158/[Electroacupuncture_alleviated_incisional_neck_pain_possibly_by_suppressing_TNF_α_expression_and_increasing_IL_4/IL_4_receptor_signaling_in_cervical_dorsal_part_of_spinal_cord_in_incisio_nal_neck_pain_rats]_ DB - PRIME DP - Unbound Medicine ER -