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Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass.
J Therm Biol. 2019 Oct; 85:102422.JT

Abstract

The responses of European sea bass to temperature increase and salinity decrease were investigated measuring mRNA expression levels of main genes involved in ion transport. Juvenile fish were pre-acclimated to seawater (SW) at 18 °C (temperate) or 24 °C (warm) for two weeks and then transferred for two weeks to either fresh water (FW) or SW at the respective temperature. Unlike temperate conditions, there is no change in Na+/K+-ATPase α1a (nka α1a) and Na+/H+ exchanger 3 (nhe3) mRNA expression following FW transfer in warm conditions. This is linked to the high expression of these genes in warm SW compared to temperate SW. Na+/Cl--cotransporter (ncc2a) expression however is increased following FW transfer in temperate and warm conditions. Main transporters involved in ion excretion (Na+/K+/2Cl--1 cotransporter, nkcc1 and cystic fibrosis transmembrane conductance regulator, cftr) as well as nitrogen excretion (Rh-glycoproteins, rhcg1 and rhbg) and acid-base regulation (V-H+-ATPase, vha-a and b) are highly expressed in SW warm conditions vs FW warm. Overall, our results suggest a higher activation of ion transport processes in warm conditions and more strikingly in SW. This is linked to a strong interplay between diverse ion transporters in order to coordinate physiological responses at the gill level.

Authors+Show Affiliations

Univ Montpellier, CNRS, IFREMER, IRD, UM, MARBEC, France.Univ Montpellier, CNRS, IFREMER, IRD, UM, MARBEC, France.Univ Montpellier, CNRS, IFREMER, IRD, UM, MARBEC, France.Centre Scientifique de Monaco, Marine Biology Department, 8 Quai Antoine 1er, 98000, Monaco.Centre Scientifique de Monaco, Marine Biology Department, 8 Quai Antoine 1er, 98000, Monaco.Univ Montpellier, CNRS, IFREMER, IRD, UM, MARBEC, France. Electronic address: catherine.lorin@umontpellier.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31657763

Citation

Masroor, Waliullah, et al. "Effect of Salinity and Temperature On the Expression of Genes Involved in Branchial Ion Transport Processes in European Sea Bass." Journal of Thermal Biology, vol. 85, 2019, p. 102422.
Masroor W, Farcy E, Blondeau-Bidet E, et al. Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass. J Therm Biol. 2019;85:102422.
Masroor, W., Farcy, E., Blondeau-Bidet, E., Venn, A., Tambutté, E., & Lorin-Nebel, C. (2019). Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass. Journal of Thermal Biology, 85, 102422. https://doi.org/10.1016/j.jtherbio.2019.102422
Masroor W, et al. Effect of Salinity and Temperature On the Expression of Genes Involved in Branchial Ion Transport Processes in European Sea Bass. J Therm Biol. 2019;85:102422. PubMed PMID: 31657763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass. AU - Masroor,Waliullah, AU - Farcy,Emilie, AU - Blondeau-Bidet,Eva, AU - Venn,Alexander, AU - Tambutté,Eric, AU - Lorin-Nebel,Catherine, Y1 - 2019/09/20/ PY - 2019/04/08/received PY - 2019/09/05/revised PY - 2019/09/17/accepted PY - 2019/10/29/entrez PY - 2019/10/29/pubmed PY - 2020/3/19/medline KW - Dicentrarchus labrax KW - Gill KW - Ion transporter KW - Salinity KW - Thermal acclimation KW - mRNA expression SP - 102422 EP - 102422 JF - Journal of thermal biology JO - J Therm Biol VL - 85 N2 - The responses of European sea bass to temperature increase and salinity decrease were investigated measuring mRNA expression levels of main genes involved in ion transport. Juvenile fish were pre-acclimated to seawater (SW) at 18 °C (temperate) or 24 °C (warm) for two weeks and then transferred for two weeks to either fresh water (FW) or SW at the respective temperature. Unlike temperate conditions, there is no change in Na+/K+-ATPase α1a (nka α1a) and Na+/H+ exchanger 3 (nhe3) mRNA expression following FW transfer in warm conditions. This is linked to the high expression of these genes in warm SW compared to temperate SW. Na+/Cl--cotransporter (ncc2a) expression however is increased following FW transfer in temperate and warm conditions. Main transporters involved in ion excretion (Na+/K+/2Cl--1 cotransporter, nkcc1 and cystic fibrosis transmembrane conductance regulator, cftr) as well as nitrogen excretion (Rh-glycoproteins, rhcg1 and rhbg) and acid-base regulation (V-H+-ATPase, vha-a and b) are highly expressed in SW warm conditions vs FW warm. Overall, our results suggest a higher activation of ion transport processes in warm conditions and more strikingly in SW. This is linked to a strong interplay between diverse ion transporters in order to coordinate physiological responses at the gill level. SN - 0306-4565 UR - https://www.unboundmedicine.com/medline/citation/31657763/Effect_of_salinity_and_temperature_on_the_expression_of_genes_involved_in_branchial_ion_transport_processes_in_European_sea_bass_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4565(19)30195-0 DB - PRIME DP - Unbound Medicine ER -