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Reoxygenation injury in isolated hepatocytes: cell death precedes conversion of xanthine dehydrogenase to xanthine oxidase.
Biochem Biophys Res Commun. 1988 Aug 30; 155(1):278-82.BB

Abstract

Reoxygenation of isolated hepatocytes from fed rats after 3 h of anaerobic incubation led to a significantly enhanced loss of cell viability. No evidence for the participation of reactive oxygen species generated by xanthine oxidase in this reoxygenation injury was found. Conversion of xanthine dehydrogenase to xanthine oxidase occurred at a time when almost all of the hepatocytes had lost their viability. Furthermore, xanthine dehydrogenase was first released from the severely injured cells and then converted to the oxidase form. The results suggest that in the intact organ participation of reactive oxygen species, generated by xanthine oxidase, in reoxygenation injury may only occur when, upon reoxygenation, hypoxic cell injury in part of the tissue has progressed to such an extent that there is a significant conversion of xanthine dehydrogenase to xanthine oxidase.

Authors+Show Affiliations

Institut für Physiologische Chemie I, Universität Düsseldorf, F.R.G.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3166376

Citation

de Groot, H, and A Littauer. "Reoxygenation Injury in Isolated Hepatocytes: Cell Death Precedes Conversion of Xanthine Dehydrogenase to Xanthine Oxidase." Biochemical and Biophysical Research Communications, vol. 155, no. 1, 1988, pp. 278-82.
de Groot H, Littauer A. Reoxygenation injury in isolated hepatocytes: cell death precedes conversion of xanthine dehydrogenase to xanthine oxidase. Biochem Biophys Res Commun. 1988;155(1):278-82.
de Groot, H., & Littauer, A. (1988). Reoxygenation injury in isolated hepatocytes: cell death precedes conversion of xanthine dehydrogenase to xanthine oxidase. Biochemical and Biophysical Research Communications, 155(1), 278-82.
de Groot H, Littauer A. Reoxygenation Injury in Isolated Hepatocytes: Cell Death Precedes Conversion of Xanthine Dehydrogenase to Xanthine Oxidase. Biochem Biophys Res Commun. 1988 Aug 30;155(1):278-82. PubMed PMID: 3166376.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reoxygenation injury in isolated hepatocytes: cell death precedes conversion of xanthine dehydrogenase to xanthine oxidase. AU - de Groot,H, AU - Littauer,A, PY - 1988/8/30/pubmed PY - 1988/8/30/medline PY - 1988/8/30/entrez SP - 278 EP - 82 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 155 IS - 1 N2 - Reoxygenation of isolated hepatocytes from fed rats after 3 h of anaerobic incubation led to a significantly enhanced loss of cell viability. No evidence for the participation of reactive oxygen species generated by xanthine oxidase in this reoxygenation injury was found. Conversion of xanthine dehydrogenase to xanthine oxidase occurred at a time when almost all of the hepatocytes had lost their viability. Furthermore, xanthine dehydrogenase was first released from the severely injured cells and then converted to the oxidase form. The results suggest that in the intact organ participation of reactive oxygen species, generated by xanthine oxidase, in reoxygenation injury may only occur when, upon reoxygenation, hypoxic cell injury in part of the tissue has progressed to such an extent that there is a significant conversion of xanthine dehydrogenase to xanthine oxidase. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/3166376/Reoxygenation_injury_in_isolated_hepatocytes:_cell_death_precedes_conversion_of_xanthine_dehydrogenase_to_xanthine_oxidase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(88)81080-5 DB - PRIME DP - Unbound Medicine ER -