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Synthesis and hemocompatibility of amino (di-) butyldeoxy modified hydroxyethyl starch.

Abstract

Cationic polymers are of interest as the basis for obtaining various biomaterials. Hydrophilic biopolymers and their modification products are of main interest. Biocompatibility is the prime criterion that makes this material usable for said purposes. In this research, hydroxyethyl starch (HES) was used as a basis for synthesis of aminodeoxy derivatives, containing n- butylamin (BA) and dibutylamin (DBA) fragments. Bromodeoxy HES was an intermediate compound. The structure of synthesized polymers was confirmed with NMR, elemental analysis and FTIR methods. The derivatives with 0.6 and 0.9 degree of substitution were tested for compatibility with blood. The research showed that HES base does not have an anticoagulation activity, does not affect human platelet aggregation and in concentrations up to 10 mg/mL of cell suspension in a buffer solution does not destroy red blood cell membrane, and therefore can be used as a component of drug delivery systems. Addition of aminodeoxy derivatives of HES hindered development of ADP-induced platelet aggregation. Derivatives of HES-DBA and HES-BA0.9 may also be of interest, but their concentration must not exceed 1*10-5 mg/mL of blood. Biodegradation of HES cationic derivatives were analyzed through identification of reducing sugars after treatment with amylase and pancreatin.

Authors+Show Affiliations

Institute of Chemistry of Federal Research Center "Komi Science Centre of the Ural Branch of the Russian Academy of Sciences", 48 Pervomayskaya, 167000 Syktyvkar, Komi Republic, Russian Federation.National Research Center for Hematology of the Ministry of Healthcare of the Russian Federation, Moscow, Russian Federation.Institute of Biology of Federal Research Center "Komi Science Centre of the Ural Branch of the Russian Academy of Sciences", 28 Kommunisticheskaya, 167982 Syktyvkar, Komi Republic, Russian Federation.Institute of Chemistry of Federal Research Center "Komi Science Centre of the Ural Branch of the Russian Academy of Sciences", 48 Pervomayskaya, 167000 Syktyvkar, Komi Republic, Russian Federation. Electronic address: udoratina-ev@chemi.komisc.ru.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31669272

Citation

Torlopov, Mikhail A., et al. "Synthesis and Hemocompatibility of Amino (di-) Butyldeoxy Modified Hydroxyethyl Starch." International Journal of Biological Macromolecules, 2019.
Torlopov MA, Drozd NN, Tarabukin DV, et al. Synthesis and hemocompatibility of amino (di-) butyldeoxy modified hydroxyethyl starch. Int J Biol Macromol. 2019.
Torlopov, M. A., Drozd, N. N., Tarabukin, D. V., & Udoratina, E. V. (2019). Synthesis and hemocompatibility of amino (di-) butyldeoxy modified hydroxyethyl starch. International Journal of Biological Macromolecules, doi:10.1016/j.ijbiomac.2019.09.184.
Torlopov MA, et al. Synthesis and Hemocompatibility of Amino (di-) Butyldeoxy Modified Hydroxyethyl Starch. Int J Biol Macromol. 2019 Oct 24; PubMed PMID: 31669272.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis and hemocompatibility of amino (di-) butyldeoxy modified hydroxyethyl starch. AU - Torlopov,Mikhail A, AU - Drozd,Natalia N, AU - Tarabukin,Dmitriy V, AU - Udoratina,Elena V, Y1 - 2019/10/24/ PY - 2019/07/02/received PY - 2019/09/27/revised PY - 2019/09/30/accepted PY - 2019/11/1/entrez KW - Aminodeoxypolysaccharides KW - Anticoagulation activity KW - Biodegradation KW - Hemocompatibility KW - Hydroxyethyl starch KW - RBS Hemolysis JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. N2 - Cationic polymers are of interest as the basis for obtaining various biomaterials. Hydrophilic biopolymers and their modification products are of main interest. Biocompatibility is the prime criterion that makes this material usable for said purposes. In this research, hydroxyethyl starch (HES) was used as a basis for synthesis of aminodeoxy derivatives, containing n- butylamin (BA) and dibutylamin (DBA) fragments. Bromodeoxy HES was an intermediate compound. The structure of synthesized polymers was confirmed with NMR, elemental analysis and FTIR methods. The derivatives with 0.6 and 0.9 degree of substitution were tested for compatibility with blood. The research showed that HES base does not have an anticoagulation activity, does not affect human platelet aggregation and in concentrations up to 10 mg/mL of cell suspension in a buffer solution does not destroy red blood cell membrane, and therefore can be used as a component of drug delivery systems. Addition of aminodeoxy derivatives of HES hindered development of ADP-induced platelet aggregation. Derivatives of HES-DBA and HES-BA0.9 may also be of interest, but their concentration must not exceed 1*10-5 mg/mL of blood. Biodegradation of HES cationic derivatives were analyzed through identification of reducing sugars after treatment with amylase and pancreatin. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/31669272/Synthesis_and_hemocompatibility_of_amino_(di-)_butyldeoxy_modified_hydroxyethyl_starch L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(19)35017-2 DB - PRIME DP - Unbound Medicine ER -