TY - JOUR T1 - Antioxidant, Xanthine Oxidase, α-Amylase and α-Glucosidase Inhibitory Activities of Bioactive Compounds from Rumex crispus L. Root. AU - Minh,Truong Ngoc, AU - Van,Truong Mai, AU - Andriana,Yusuf, AU - Vinh,Le The, AU - Hau,Dang Viet, AU - Duyen,Dang Hong, AU - Guzman-Gelani,Chona de, Y1 - 2019/10/29/ PY - 2019/09/19/received PY - 2019/10/19/revised PY - 2019/10/22/accepted PY - 2019/11/2/entrez PY - 2019/11/2/pubmed PY - 2020/3/21/medline KW - Rumex crispus KW - anti-diabetic KW - anti-gout KW - anti-radical KW - bioactive compound KW - chrysophanol KW - physcion JF - Molecules (Basel, Switzerland) JO - Molecules VL - 24 IS - 21 N2 - The root of Rumex crispus L. has been shown to possess anti-gout and anti-diabetic properties, but the compounds responsible for these pharmaceutical effects have not yet been reported. In this study, we aimed to isolate and purify active components from the root of R. crispus, and to evaluate their anti-radical, anti-gout and anti-diabetic capacities. From the ethyl acetate (EtOAc) extract, two compounds, chrysophanol (1) and physcion (2), were isolated by column chromatography with an elution of hexane and EtOAc at a 9:1 ratio. Their structures were identified by spectrometric techniques including gas chromatography-mass spectrometry (GC-MS), electrospray ionization-mass spectrometry (ESI-MS), X-ray diffraction analyses and nuclear magnetic resonance (NMR). The results of bioassays indicated that (1) showed stronger activities than (2). For antioxidant activity, (1) and (2) exhibited remarkable DPPH radical scavenging capacity (IC50 = 9.8 and 12.1 µg/mL), which was about two times stronger than BHT (IC50 = 19.4 µg/mL). The anti-gout property of (1) and (2) were comparable to the positive control allopurinol, these compounds exerted strong inhibition against the activity of xanthine oxidase (IC50 = 36.4 and 45.0 µg/mL, respectively). In the anti-diabetic assay, (1) and (2) displayed considerable inhibitory ability on α-glucosidase, their IC50 values (IC50 = 20.1 and 18.9 µg/mL, respectively) were higher than that of standard acarbose (IC50 = 143.4 µg/mL). Findings of this study highlight that (1) and (2) may be promising agents to treat gout and diabetes, which may greatly contribute to the medicinal properties of Rumex crispus root. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/31671906/Antioxidant_Xanthine_Oxidase_α_Amylase_and_α_Glucosidase_Inhibitory_Activities_of_Bioactive_Compounds_from_Rumex_crispus_L__Root_ DB - PRIME DP - Unbound Medicine ER -