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ICP-MS and HPLC analyses, enzyme inhibition and antioxidant potential of Achillea schischkinii Sosn.
Bioorg Chem 2020; 94:103333BC

Abstract

Achillea schischkinii Sosn. is an endemic plant species and it belongs to Asteraceae family. It is distributed widely in the Central and East Anatolia. This study was carried out for evaluation of the antioxidant activity, enzyme inhibition effect, elemental and phenolic content of A. schischkinii. Briefly, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-glycosidase (α-Gly), and glutathione S-transferase (GST) enzymes were strongly inhibited by A. schischkinii. IC50 values for AChE, BChE, α-Gly, and GST enzymes were found as 19.3 mg/mL, 15.4 mg/mL, 69.3 mg/mL, and 34.7 mg/mL respectively. The antioxidant activity of the sample was evaluated by four different in vitro bioanalytical methods. Besides, the concentrations of twelve elements in A. schischkinii were analyzed by ICP-MS technique. Zn (50.6 ppm), Mn (23.0 ppm), and Cu (12.7 ppm) were found as major elements. Furthermore, catechin (20.8 µg/mg extract), trans-ferulic acid (18.3 µg/mg extract), and gallic acid (11.2 µg/mg extract) were characterized as major phenolic compounds by using HPLC. PRACTICAL APPLICATIONS: Acetylcholinesterase, butyrylcholinesterase, α-glycosidase, and glutathione s-transferase enzymes have crucial functions on metabolism. Enzyme inhibition or activation mostly attributed to some health disorders such as Alzheimer's disease, Diabetes mellitus, cancer and hyperglycemia. Phenolic contents are responsible for effective biological activity. This study evaluated the phenolic content and antioxidant activity of Achillea schischkinii as well as the inhibition effect against four metabolic enzymes. The results would be beneficial for using the plant in the food industry and pharmacological process.

Authors+Show Affiliations

Department of Medical Services and Techniques, Health Services Vocational School, Igdır University, Igdır, Turkey.Department of Biochemistry, Faculty of Science and Arts, Igdır University, Igdır, Turkey.Department of Biochemistry, Faculty of Science and Arts, Igdır University, Igdır, Turkey.Department of Chemistry, Faculty of Science, Ataturk University, Erzurum, Turkey.Department of Nursing, School of Health, Mus Alparslan University, Mus, Turkey. Electronic address: e.bursal@alparslan.edu.tr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31677859

Citation

Türkan, Fikret, et al. "ICP-MS and HPLC Analyses, Enzyme Inhibition and Antioxidant Potential of Achillea Schischkinii Sosn." Bioorganic Chemistry, vol. 94, 2020, p. 103333.
Türkan F, Atalar MN, Aras A, et al. ICP-MS and HPLC analyses, enzyme inhibition and antioxidant potential of Achillea schischkinii Sosn. Bioorg Chem. 2020;94:103333.
Türkan, F., Atalar, M. N., Aras, A., Gülçin, İ., & Bursal, E. (2020). ICP-MS and HPLC analyses, enzyme inhibition and antioxidant potential of Achillea schischkinii Sosn. Bioorganic Chemistry, 94, p. 103333. doi:10.1016/j.bioorg.2019.103333.
Türkan F, et al. ICP-MS and HPLC Analyses, Enzyme Inhibition and Antioxidant Potential of Achillea Schischkinii Sosn. Bioorg Chem. 2020;94:103333. PubMed PMID: 31677859.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ICP-MS and HPLC analyses, enzyme inhibition and antioxidant potential of Achillea schischkinii Sosn. AU - Türkan,Fikret, AU - Atalar,Mehmet Nuri, AU - Aras,Abdülmelik, AU - Gülçin,İlhami, AU - Bursal,Ercan, Y1 - 2019/10/01/ PY - 2019/06/19/received PY - 2019/09/27/revised PY - 2019/09/29/accepted PY - 2019/11/5/pubmed PY - 2019/11/5/medline PY - 2019/11/4/entrez KW - Achillea schischkinii KW - Antioxidant KW - Elemental analysis KW - Enzymes KW - Phenolic compounds SP - 103333 EP - 103333 JF - Bioorganic chemistry JO - Bioorg. Chem. VL - 94 N2 - Achillea schischkinii Sosn. is an endemic plant species and it belongs to Asteraceae family. It is distributed widely in the Central and East Anatolia. This study was carried out for evaluation of the antioxidant activity, enzyme inhibition effect, elemental and phenolic content of A. schischkinii. Briefly, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-glycosidase (α-Gly), and glutathione S-transferase (GST) enzymes were strongly inhibited by A. schischkinii. IC50 values for AChE, BChE, α-Gly, and GST enzymes were found as 19.3 mg/mL, 15.4 mg/mL, 69.3 mg/mL, and 34.7 mg/mL respectively. The antioxidant activity of the sample was evaluated by four different in vitro bioanalytical methods. Besides, the concentrations of twelve elements in A. schischkinii were analyzed by ICP-MS technique. Zn (50.6 ppm), Mn (23.0 ppm), and Cu (12.7 ppm) were found as major elements. Furthermore, catechin (20.8 µg/mg extract), trans-ferulic acid (18.3 µg/mg extract), and gallic acid (11.2 µg/mg extract) were characterized as major phenolic compounds by using HPLC. PRACTICAL APPLICATIONS: Acetylcholinesterase, butyrylcholinesterase, α-glycosidase, and glutathione s-transferase enzymes have crucial functions on metabolism. Enzyme inhibition or activation mostly attributed to some health disorders such as Alzheimer's disease, Diabetes mellitus, cancer and hyperglycemia. Phenolic contents are responsible for effective biological activity. This study evaluated the phenolic content and antioxidant activity of Achillea schischkinii as well as the inhibition effect against four metabolic enzymes. The results would be beneficial for using the plant in the food industry and pharmacological process. SN - 1090-2120 UR - https://www.unboundmedicine.com/medline/citation/31677859/ICP-MS_and_HPLC_analyses,_enzyme_inhibition_and_antioxidant_potential_of_Achillea_schischkinii_Sosn L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-2068(19)30982-4 DB - PRIME DP - Unbound Medicine ER -