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Comparative study of host-associated and commercial probiotic effects on serum and mucosal immune parameters, intestinal microbiota, digestive enzymes activity and growth performance of roach (Rutilus rutilus caspicus) fingerlings.
Fish Shellfish Immunol. 2020 Mar; 98:661-669.FS

Abstract

The study aimed to isolate host-associated probiotic (HAP) lactic acid bacteria from intestine of adult Caspian roach and compare the efficacy of HAP with a commercially available probiotic strain (Pediococcus acidilactici) on the growth and feed utilisation, digestive enzymes and systemic and mucosal immune system of roach fingerling. The HAP strain isolated from roach intestine was Enterococcus faecium strain CGMCC1.2136. The experiment was a simple completely randomized design and lasted for eight weeks. Two hundred and seventy fish with an average weight of 12 g randomly distributed into nine tanks. The trial consisted of three treatments with three respective replications. During the experimental period, fish received basal diet without any bacterial supplementation (as the control group), basal diet enriched with 108 CFU g-1 HAP or 107 CFU g-1 CP. At the end of the experiment, serum immune parameters of those fish fed HAP including alkaline phosphatase activity, total protein content, total immunoglobulin level, lysozyme activity and complement activity (ACH50) were significantly higher that other experimental groups (P < 0.05). Similarly, dietary supplementation of HAP resulted in better mucosal immune parameters in comparison to control group and commercial probiotic administration (P < 0.05). Intestinal heterotrophic bacteria and autochthonous LAB counts of those fish fed HAP were significantly higher than other experimental groups at the end of the experiment as well as 15 days seizing probiotic administrations (P < 0.05). Fish fed with HAP containing diet presented significantly higher amylase, lipase and protease activity in comparison to the CP fed fish and the control group (P < 0.05). Growth indices of those fish fed HAP were significantly higher than other treatments (P < 0.05). The highest carcass protein and ash content along with the lowest body moisture content belonged to those fish received HAP (P < 0.05). In conclusion, the use host-HAP resulted in better immune competence and growth performance and it seems aquaculture sector should probably focus on the development of probiotics isolated from the cultured species instead of using terrestrial probiotics with greatly different requirements and environmental conditions.

Authors+Show Affiliations

Department of Fisheries, Faculty of Agriculture and Natural Resources, Urmia University, Iran.Department of Fisheries, Faculty of Agriculture and Natural Resources, Urmia University, Iran.Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Iran. Electronic address: hoseinifar@gau.ac.ir.Department of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Iran.Department of Fisheries, Faculty of Agriculture and Natural Resources, Urmia University, Iran.Department of Fisheries, Faculty of Agriculture and Natural Resources, Urmia University, Iran.Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand.ISEM, IRD, CNRS, EPHE, Institute of Evolution of Montpellier, Université de Montpellier, 34090, Montpellier, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31678185

Citation

Tarkhani, Reza, et al. "Comparative Study of Host-associated and Commercial Probiotic Effects On Serum and Mucosal Immune Parameters, Intestinal Microbiota, Digestive Enzymes Activity and Growth Performance of Roach (Rutilus Rutilus Caspicus) Fingerlings." Fish & Shellfish Immunology, vol. 98, 2020, pp. 661-669.
Tarkhani R, Imani A, Hoseinifar SH, et al. Comparative study of host-associated and commercial probiotic effects on serum and mucosal immune parameters, intestinal microbiota, digestive enzymes activity and growth performance of roach (Rutilus rutilus caspicus) fingerlings. Fish Shellfish Immunol. 2020;98:661-669.
Tarkhani, R., Imani, A., Hoseinifar, S. H., Ashayerizadeh, O., Sarvi Moghanlou, K., Manaffar, R., Van Doan, H., & Reverter, M. (2020). Comparative study of host-associated and commercial probiotic effects on serum and mucosal immune parameters, intestinal microbiota, digestive enzymes activity and growth performance of roach (Rutilus rutilus caspicus) fingerlings. Fish & Shellfish Immunology, 98, 661-669. https://doi.org/10.1016/j.fsi.2019.10.063
Tarkhani R, et al. Comparative Study of Host-associated and Commercial Probiotic Effects On Serum and Mucosal Immune Parameters, Intestinal Microbiota, Digestive Enzymes Activity and Growth Performance of Roach (Rutilus Rutilus Caspicus) Fingerlings. Fish Shellfish Immunol. 2020;98:661-669. PubMed PMID: 31678185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative study of host-associated and commercial probiotic effects on serum and mucosal immune parameters, intestinal microbiota, digestive enzymes activity and growth performance of roach (Rutilus rutilus caspicus) fingerlings. AU - Tarkhani,Reza, AU - Imani,Ahmad, AU - Hoseinifar,Seyed Hossein, AU - Ashayerizadeh,Omid, AU - Sarvi Moghanlou,Kourosh, AU - Manaffar,Ramin, AU - Van Doan,Hien, AU - Reverter,Miriam, Y1 - 2019/10/31/ PY - 2019/05/30/received PY - 2019/10/10/revised PY - 2019/10/28/accepted PY - 2019/11/5/pubmed PY - 2020/10/8/medline PY - 2019/11/4/entrez KW - Enterococcus faecium KW - Host-associated probiotic KW - Non-specific immunity KW - Roach SP - 661 EP - 669 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 98 N2 - The study aimed to isolate host-associated probiotic (HAP) lactic acid bacteria from intestine of adult Caspian roach and compare the efficacy of HAP with a commercially available probiotic strain (Pediococcus acidilactici) on the growth and feed utilisation, digestive enzymes and systemic and mucosal immune system of roach fingerling. The HAP strain isolated from roach intestine was Enterococcus faecium strain CGMCC1.2136. The experiment was a simple completely randomized design and lasted for eight weeks. Two hundred and seventy fish with an average weight of 12 g randomly distributed into nine tanks. The trial consisted of three treatments with three respective replications. During the experimental period, fish received basal diet without any bacterial supplementation (as the control group), basal diet enriched with 108 CFU g-1 HAP or 107 CFU g-1 CP. At the end of the experiment, serum immune parameters of those fish fed HAP including alkaline phosphatase activity, total protein content, total immunoglobulin level, lysozyme activity and complement activity (ACH50) were significantly higher that other experimental groups (P < 0.05). Similarly, dietary supplementation of HAP resulted in better mucosal immune parameters in comparison to control group and commercial probiotic administration (P < 0.05). Intestinal heterotrophic bacteria and autochthonous LAB counts of those fish fed HAP were significantly higher than other experimental groups at the end of the experiment as well as 15 days seizing probiotic administrations (P < 0.05). Fish fed with HAP containing diet presented significantly higher amylase, lipase and protease activity in comparison to the CP fed fish and the control group (P < 0.05). Growth indices of those fish fed HAP were significantly higher than other treatments (P < 0.05). The highest carcass protein and ash content along with the lowest body moisture content belonged to those fish received HAP (P < 0.05). In conclusion, the use host-HAP resulted in better immune competence and growth performance and it seems aquaculture sector should probably focus on the development of probiotics isolated from the cultured species instead of using terrestrial probiotics with greatly different requirements and environmental conditions. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/31678185/Comparative_study_of_host_associated_and_commercial_probiotic_effects_on_serum_and_mucosal_immune_parameters_intestinal_microbiota_digestive_enzymes_activity_and_growth_performance_of_roach__Rutilus_rutilus_caspicus__fingerlings_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(19)31031-9 DB - PRIME DP - Unbound Medicine ER -