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Screening for differentially expressed circRNA between Kashin-Beck disease and osteoarthritis patients based on circRNA chips.
Clin Chim Acta. 2020 Feb; 501:92-101.CC

Abstract

OBJECTIVE

This research aims to explore differentially expressed circRNA between OA and KBD and potential diagnostic biomarkers.

METHODS

Total RNA was extracted from 5 pairs of KBD and OA knee joint cartilage specimens, and the expression of circRNAs was analyzed by Chip Scanning Analysis. The microarray data was verified by quantitative polymerase chain reaction (qRT-PCR). CircRNA-miRNA network was constructed to predict targeting microRNAs of circRNA genes. Peripheral blood samples from 25 KBD patients and 25 OA patients were collected for verification by qRT-PCR. Diagnostic value was evaluated by the area under the receiver operator characteristic (ROC) curve.

RESULTS

A total of 1627 circRNAs were differentially expressed between OA and KBD. Five bone and joint disease-related circRNAs were chosen for qRT-PCR validation. The difference in expression profile of hsa_circRNA_0020014 was confirmed by qRT-PCR, and its circRNA-miRNA regulation network was set up. The ROC curve demonstrated that hsa_circ_0020014_CBC1 in peripheral blood could distinguish patients with KBD and OA.

CONCLUSION

The expression profiles of circRNA were significantly different between OA and KBD. hsa_circRNA_0020014 is a potential biomarker for differential diagnosis between these two diseases.

Authors+Show Affiliations

Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiao Tong University, No. 277 Yanta West Road, Xi'an, Shaanxi 710061, People's Republic of China.School of Public Health, Health Science Center of Xi'an Jiaotong University, NHC Key Laboratory of Trace Elements and Endemic Diseases (Xi'an Jiaotong University), Xi'an, Shaanxi No. 76 Yanta West Road, Xi'an 710061, People's Republic of China.School of Public Health, Health Science Center of Xi'an Jiaotong University, NHC Key Laboratory of Trace Elements and Endemic Diseases (Xi'an Jiaotong University), Xi'an, Shaanxi No. 76 Yanta West Road, Xi'an 710061, People's Republic of China.Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiao Tong University, No. 277 Yanta West Road, Xi'an, Shaanxi 710061, People's Republic of China.Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiao Tong University, No. 277 Yanta West Road, Xi'an, Shaanxi 710061, People's Republic of China.School of Public Health, Health Science Center of Xi'an Jiaotong University, NHC Key Laboratory of Trace Elements and Endemic Diseases (Xi'an Jiaotong University), Xi'an, Shaanxi No. 76 Yanta West Road, Xi'an 710061, People's Republic of China; Department of Integrative Medical Biology, University of Umeå, Umeå, Sweden. Electronic address: mikko.lammi@umu.se.School of Public Health, Health Science Center of Xi'an Jiaotong University, NHC Key Laboratory of Trace Elements and Endemic Diseases (Xi'an Jiaotong University), Xi'an, Shaanxi No. 76 Yanta West Road, Xi'an 710061, People's Republic of China. Electronic address: guox@xjtu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31678276

Citation

Wang, Ying, et al. "Screening for Differentially Expressed circRNA Between Kashin-Beck Disease and Osteoarthritis Patients Based On circRNA Chips." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 501, 2020, pp. 92-101.
Wang Y, Wu C, Zhang Y, et al. Screening for differentially expressed circRNA between Kashin-Beck disease and osteoarthritis patients based on circRNA chips. Clin Chim Acta. 2020;501:92-101.
Wang, Y., Wu, C., Zhang, Y., Yang, Y., Ren, Z., Lammi, M. J., & Guo, X. (2020). Screening for differentially expressed circRNA between Kashin-Beck disease and osteoarthritis patients based on circRNA chips. Clinica Chimica Acta; International Journal of Clinical Chemistry, 501, 92-101. https://doi.org/10.1016/j.cca.2019.10.026
Wang Y, et al. Screening for Differentially Expressed circRNA Between Kashin-Beck Disease and Osteoarthritis Patients Based On circRNA Chips. Clin Chim Acta. 2020;501:92-101. PubMed PMID: 31678276.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening for differentially expressed circRNA between Kashin-Beck disease and osteoarthritis patients based on circRNA chips. AU - Wang,Ying, AU - Wu,Cuiyan, AU - Zhang,Yanan, AU - Yang,Yimin, AU - Ren,Zhiwei, AU - Lammi,Mikko J, AU - Guo,Xiong, Y1 - 2019/10/31/ PY - 2019/09/14/received PY - 2019/10/22/accepted PY - 2019/11/5/pubmed PY - 2020/7/30/medline PY - 2019/11/4/entrez KW - Biomarkers KW - Kashin–Beck disease KW - Osteoarthritis KW - circRNAs expression profiles SP - 92 EP - 101 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin. Chim. Acta VL - 501 N2 - OBJECTIVE: This research aims to explore differentially expressed circRNA between OA and KBD and potential diagnostic biomarkers. METHODS: Total RNA was extracted from 5 pairs of KBD and OA knee joint cartilage specimens, and the expression of circRNAs was analyzed by Chip Scanning Analysis. The microarray data was verified by quantitative polymerase chain reaction (qRT-PCR). CircRNA-miRNA network was constructed to predict targeting microRNAs of circRNA genes. Peripheral blood samples from 25 KBD patients and 25 OA patients were collected for verification by qRT-PCR. Diagnostic value was evaluated by the area under the receiver operator characteristic (ROC) curve. RESULTS: A total of 1627 circRNAs were differentially expressed between OA and KBD. Five bone and joint disease-related circRNAs were chosen for qRT-PCR validation. The difference in expression profile of hsa_circRNA_0020014 was confirmed by qRT-PCR, and its circRNA-miRNA regulation network was set up. The ROC curve demonstrated that hsa_circ_0020014_CBC1 in peripheral blood could distinguish patients with KBD and OA. CONCLUSION: The expression profiles of circRNA were significantly different between OA and KBD. hsa_circRNA_0020014 is a potential biomarker for differential diagnosis between these two diseases. SN - 1873-3492 UR - https://www.unboundmedicine.com/medline/citation/31678276/Screening_for_differentially_expressed_circRNA_between_Kashin-Beck_disease_and_osteoarthritis_patients_based_on_circRNA_chips L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-8981(19)32092-3 DB - PRIME DP - Unbound Medicine ER -