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Elevated level of Galectin-1 in bronchoalveolar lavage of patients with idiopathic pulmonary fibrosis.
Respir Physiol Neurobiol. 2020 02; 273:103323.RP

Abstract

RATIONALE

Galectin-1 is a carbohydrate-binding protein involved in apoptosis, cell-proliferation and differentiation, implicated in T-cell homeostasis and survival. The aim of the present study was to determine concentrations of galectin-1 in BAL fluid from patients with IPF and other interstitial lung diseases in order to validate proteomic previous findings.

METHODS

36 IPF patients (16 females, mean age of 64.8 ± 8.9 years), 24 sarcoidosis patients (15 females, mean age of 56.3 ± 13.4), 7 interstitial lung diseases associated to systemic sclerosis (ILD-SSc) patients (5 females, mean age of 55.5 ± 16.4) and six healthy controls (4 females, mean age 47.8 ± 15.2) were included. Galectin-1 concentrations were determined in BAL samples by an ELISA assay.

RESULTS

Galectin-1 concentrations were significantly higher in BAL of IPF patients than in sarcoidosis and ILD-SSc patients and healthy controls. In IPF patients, galectin-1 levels showed significant inverse correlations with DLCO%, KCO% and BAL lymphocyte percentages and a positive correlation with BAL macrophage percentages. Former IPF smokers had higher concentrations of this protein compared with non-smoker IPF patients.

CONCLUSION

Galectin-1 was confirmed a protein of interest in idiopathic pulmonary fibrosis. Its BAL concentrations were higher in IPF patients than in controls and correlated with disease severity. Galectin-1 was suggested to have a role in the pathogenesis of IPF, principally through the ERK/MAPK pathway and the inhibition of galectin-1 is a potential therapeutic target worthy of research.

Authors+Show Affiliations

Respiratory Diseases Unit, Department of Medical, Surgical and Neurological Sciences, University of Siena, Italy; University Hospital of Siena (Azienda Ospedaliera Universitaria Senese, AOUS), Siena, Italy. Electronic address: david.btt@gmail.com.Respiratory Diseases Unit, Department of Medical, Surgical and Neurological Sciences, University of Siena, Italy; University Hospital of Siena (Azienda Ospedaliera Universitaria Senese, AOUS), Siena, Italy.Respiratory Diseases Unit, Department of Medical, Surgical and Neurological Sciences, University of Siena, Italy; University Hospital of Siena (Azienda Ospedaliera Universitaria Senese, AOUS), Siena, Italy.Respiratory Diseases Unit, Department of Medical, Surgical and Neurological Sciences, University of Siena, Italy; University Hospital of Siena (Azienda Ospedaliera Universitaria Senese, AOUS), Siena, Italy.Respiratory Diseases Unit, Department of Medical, Surgical and Neurological Sciences, University of Siena, Italy; University Hospital of Siena (Azienda Ospedaliera Universitaria Senese, AOUS), Siena, Italy.Respiratory Diseases Unit, Department of Medical, Surgical and Neurological Sciences, University of Siena, Italy; University Hospital of Siena (Azienda Ospedaliera Universitaria Senese, AOUS), Siena, Italy.Respiratory Diseases Unit, Department of Medical, Surgical and Neurological Sciences, University of Siena, Italy; University Hospital of Siena (Azienda Ospedaliera Universitaria Senese, AOUS), Siena, Italy.Respiratory Diseases Unit, Department of Medical, Surgical and Neurological Sciences, University of Siena, Italy; University Hospital of Siena (Azienda Ospedaliera Universitaria Senese, AOUS), Siena, Italy.Respiratory Diseases Unit, Department of Medical, Surgical and Neurological Sciences, University of Siena, Italy; University Hospital of Siena (Azienda Ospedaliera Universitaria Senese, AOUS), Siena, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31678459

Citation

Bennett, David, et al. "Elevated Level of Galectin-1 in Bronchoalveolar Lavage of Patients With Idiopathic Pulmonary Fibrosis." Respiratory Physiology & Neurobiology, vol. 273, 2020, p. 103323.
Bennett D, Bargagli E, Bianchi N, et al. Elevated level of Galectin-1 in bronchoalveolar lavage of patients with idiopathic pulmonary fibrosis. Respir Physiol Neurobiol. 2020;273:103323.
Bennett, D., Bargagli, E., Bianchi, N., Landi, C., Fossi, A., Fui, A., Sestini, P., Refini, R. M., & Rottoli, P. (2020). Elevated level of Galectin-1 in bronchoalveolar lavage of patients with idiopathic pulmonary fibrosis. Respiratory Physiology & Neurobiology, 273, 103323. https://doi.org/10.1016/j.resp.2019.103323
Bennett D, et al. Elevated Level of Galectin-1 in Bronchoalveolar Lavage of Patients With Idiopathic Pulmonary Fibrosis. Respir Physiol Neurobiol. 2020;273:103323. PubMed PMID: 31678459.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Elevated level of Galectin-1 in bronchoalveolar lavage of patients with idiopathic pulmonary fibrosis. AU - Bennett,David, AU - Bargagli,Elena, AU - Bianchi,Nicola, AU - Landi,Claudia, AU - Fossi,Antonella, AU - Fui,Annalisa, AU - Sestini,Piersante, AU - Refini,Rosa Metella, AU - Rottoli,Paola, Y1 - 2019/10/31/ PY - 2019/08/08/received PY - 2019/10/04/revised PY - 2019/10/14/accepted PY - 2019/11/5/pubmed PY - 2021/1/29/medline PY - 2019/11/4/entrez KW - Biomarker KW - Bronchoalveolar lavage KW - Galectin-1 KW - Idiopathic pulmonary fibrosis KW - Sarcoidosis KW - Systemic sclerosis SP - 103323 EP - 103323 JF - Respiratory physiology & neurobiology JO - Respir Physiol Neurobiol VL - 273 N2 - RATIONALE: Galectin-1 is a carbohydrate-binding protein involved in apoptosis, cell-proliferation and differentiation, implicated in T-cell homeostasis and survival. The aim of the present study was to determine concentrations of galectin-1 in BAL fluid from patients with IPF and other interstitial lung diseases in order to validate proteomic previous findings. METHODS: 36 IPF patients (16 females, mean age of 64.8 ± 8.9 years), 24 sarcoidosis patients (15 females, mean age of 56.3 ± 13.4), 7 interstitial lung diseases associated to systemic sclerosis (ILD-SSc) patients (5 females, mean age of 55.5 ± 16.4) and six healthy controls (4 females, mean age 47.8 ± 15.2) were included. Galectin-1 concentrations were determined in BAL samples by an ELISA assay. RESULTS: Galectin-1 concentrations were significantly higher in BAL of IPF patients than in sarcoidosis and ILD-SSc patients and healthy controls. In IPF patients, galectin-1 levels showed significant inverse correlations with DLCO%, KCO% and BAL lymphocyte percentages and a positive correlation with BAL macrophage percentages. Former IPF smokers had higher concentrations of this protein compared with non-smoker IPF patients. CONCLUSION: Galectin-1 was confirmed a protein of interest in idiopathic pulmonary fibrosis. Its BAL concentrations were higher in IPF patients than in controls and correlated with disease severity. Galectin-1 was suggested to have a role in the pathogenesis of IPF, principally through the ERK/MAPK pathway and the inhibition of galectin-1 is a potential therapeutic target worthy of research. SN - 1878-1519 UR - https://www.unboundmedicine.com/medline/citation/31678459/Elevated_level_of_Galectin_1_in_bronchoalveolar_lavage_of_patients_with_idiopathic_pulmonary_fibrosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1569-9048(19)30279-4 DB - PRIME DP - Unbound Medicine ER -