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AgNPs from Nigella sativa Control Breast Cancer: An In Vitro Study.
J Environ Pathol Toxicol Oncol. 2019; 38(2):185-194.JE

Abstract

In our current study, we synthesized silver nanoparticles (AgNPs) from an aqueous seed extract of Nigella sativa. The seed extract contains phytochemical compounds including phenols, terpenoids, and flavonoids that may act as reducing agents and are able to convert metal ions to metal nanoparticles. The formation of synthesized AgNPs was characterized using UV-visible spectroscopy, Fourier transform infra-red spectroscopy (FT-IR), scanning electron microscopy (SEM) and energy dispersive analysis of X-rays (EDX). The efficacy of N-AgNPs against human breast cancer (MCF-7) cells was tested. The synthesized AgNPs displayed dose-dependent cytotoxicity (1-200 µg/mL) against MCF-7 cells. Morphological alterations of the cells also appeared as bright field images. Treatment of synthesized AgNPs altered the expression of Bax and Bcl-2 (apoptotic proteins) and COX-2 (inflammatory marker) in MCF-7 cells. To our knowledge, this is the first report demonstrating that N-AgNPs from Nigella sativa can induce apoptosis in MCF-7 cells.

Authors+Show Affiliations

School of Life Sciences, BS Abdur Rahman Crescent Institute of Science & Technology, Chennai, India.School of Life Sciences, BS Abdur Rahman Crescent Institute of Science & Technology, Chennai, India.School of Life Sciences, BS Abdur Rahman Crescent Institute of Science & Technology, Chennai, India.Department of Toxicology, JamiaHamdard, New Delhi, India.Department of Toxicology, JamiaHamdard, New Delhi, India.School of Life Sciences, BS Abdur Rahman Crescent Institute of Science & Technology, Chennai, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31679281

Citation

Rohini, Baradhan, et al. "AgNPs From Nigella Sativa Control Breast Cancer: an in Vitro Study." Journal of Environmental Pathology, Toxicology and Oncology : Official Organ of the International Society for Environmental Toxicology and Cancer, vol. 38, no. 2, 2019, pp. 185-194.
Rohini B, Akther T, Waseem M, et al. AgNPs from Nigella sativa Control Breast Cancer: An In Vitro Study. J Environ Pathol Toxicol Oncol. 2019;38(2):185-194.
Rohini, B., Akther, T., Waseem, M., Khan, J., Kashif, M., & Hemalatha, S. (2019). AgNPs from Nigella sativa Control Breast Cancer: An In Vitro Study. Journal of Environmental Pathology, Toxicology and Oncology : Official Organ of the International Society for Environmental Toxicology and Cancer, 38(2), 185-194. https://doi.org/10.1615/JEnvironPatholToxicolOncol.2019027318
Rohini B, et al. AgNPs From Nigella Sativa Control Breast Cancer: an in Vitro Study. J Environ Pathol Toxicol Oncol. 2019;38(2):185-194. PubMed PMID: 31679281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - AgNPs from Nigella sativa Control Breast Cancer: An In Vitro Study. AU - Rohini,Baradhan, AU - Akther,Tahira, AU - Waseem,Mohammad, AU - Khan,Jasim, AU - Kashif,Mohammad, AU - Hemalatha,Srinivasan, PY - 2019/11/4/entrez PY - 2019/11/5/pubmed PY - 2019/11/8/medline SP - 185 EP - 194 JF - Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer JO - J Environ Pathol Toxicol Oncol VL - 38 IS - 2 N2 - In our current study, we synthesized silver nanoparticles (AgNPs) from an aqueous seed extract of Nigella sativa. The seed extract contains phytochemical compounds including phenols, terpenoids, and flavonoids that may act as reducing agents and are able to convert metal ions to metal nanoparticles. The formation of synthesized AgNPs was characterized using UV-visible spectroscopy, Fourier transform infra-red spectroscopy (FT-IR), scanning electron microscopy (SEM) and energy dispersive analysis of X-rays (EDX). The efficacy of N-AgNPs against human breast cancer (MCF-7) cells was tested. The synthesized AgNPs displayed dose-dependent cytotoxicity (1-200 µg/mL) against MCF-7 cells. Morphological alterations of the cells also appeared as bright field images. Treatment of synthesized AgNPs altered the expression of Bax and Bcl-2 (apoptotic proteins) and COX-2 (inflammatory marker) in MCF-7 cells. To our knowledge, this is the first report demonstrating that N-AgNPs from Nigella sativa can induce apoptosis in MCF-7 cells. SN - 2162-6537 UR - https://www.unboundmedicine.com/medline/citation/31679281/AgNPs_from_Nigella_sativa_Control_Breast_Cancer:_An_In_Vitro_Study_ L2 - http://www.begellhouse.com/journals/0ff459a57a4c08d0,2a1f3ff2521fee54,002de0371cd2e463.html DB - PRIME DP - Unbound Medicine ER -