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Posttranscriptional regulation of MMP-9 by HuR contributes to IL-1β-induced pterygium fibroblast migration and invasion.
J Cell Physiol. 2020 06; 235(6):5130-5140.JC

Abstract

Inflammation is considered to be critical in the pterygium progression and recurrence. However, the underlying molecular mechanism is not well understood. Herein, we investigated the potential role of RNA binding protein human antigen R (HuR) responsible for the impact of inflammation on pterygium development. The expression of HuR and matrix metallopeptidase-9 (MMP-9) in pterygium and normal conjunctiva was detected with immunohistochemistry and quantitative reverse transcription polymerase chain reaction (qRT-PCR). The influence of interleukin-1β (IL-1β) on HuR expression and cellular distribution was determined with western blot and immunofluorescence. The pterygium fibroblast (PTF) migration was determined with scratch wound healing assay and Transwell migration assay. MMP-9 production was determined with qRT-PCR and gelatin zymography. The interaction between HuR and MMP-9 was investigated with RNP immunoprecipitation (IP) followed by RT-PCR and messenger RNA (mRNA) stability analysis. HuR and MMP-9 expression are elevated in pterygium, especially progressive pterygium compared with normal conjunctiva. IL-1β could increase the expression and nucleus-cytoplasm shuttle of HuR in cultured PTFs. HuR mediated the stimulatory effect of IL-1β on PTF migration and MMP-9 production. HuR bound to MMP-9 mRNA and in turn increased it stability. Our results suggest that posttranscriptional regulation of MMP-9 via stabilizing mRNA by HuR might contribute to the stimulatory effect of inflammatory factor IL-1β on pterygium progression. These findings shed light on the pathogenesis of pterygium and provide a promising target for adjuvant treatment of pterygium.

Authors+Show Affiliations

Guangzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China. Department of Histology and Embryology, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China.Department of Ophthalmology, Guangdong Women and Children Hospital, Guangzhou, China. Institute of Ophthalmology, School of Medicine, Jinan University, Guangzhou, China.Department of Histology and Embryology, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China.Department of Ophthalmology, Guangdong No. 2 Provincial People's Hospital, Guangzhou, China. Department of Ophthalmology, Guangzhou Red Cross Hospital, The Fourth Affiliated Hospital of Jinan University, Guangzhou, China.Department of Public Health and Preventive Medicine, Jinan University, Guangzhou, China.Department of Public Health and Preventive Medicine, Jinan University, Guangzhou, China.Department of Ophthalmology, The First Affiliated Hospital, Jinan University, Guangzhou, China. Institute of Ophthalmology, School of Medicine, Jinan University, Guangzhou, China.Department of Public Health and Preventive Medicine, Jinan University, Guangzhou, China.Department of Ophthalmology, The First Affiliated Hospital, Jinan University, Guangzhou, China. Institute of Ophthalmology, School of Medicine, Jinan University, Guangzhou, China.Department of Public Health and Preventive Medicine, Jinan University, Guangzhou, China.Institute of Ophthalmology, School of Medicine, Jinan University, Guangzhou, China.Department of Public Health and Preventive Medicine, Jinan University, Guangzhou, China. Department of Ophthalmology, The First Affiliated Hospital, Jinan University, Guangzhou, China. Institute of Ophthalmology, School of Medicine, Jinan University, Guangzhou, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31691974

Citation

Cui, Yu-Hong, et al. "Posttranscriptional Regulation of MMP-9 By HuR Contributes to IL-1β-induced Pterygium Fibroblast Migration and Invasion." Journal of Cellular Physiology, vol. 235, no. 6, 2020, pp. 5130-5140.
Cui YH, Feng QY, Liu Q, et al. Posttranscriptional regulation of MMP-9 by HuR contributes to IL-1β-induced pterygium fibroblast migration and invasion. J Cell Physiol. 2020;235(6):5130-5140.
Cui, Y. H., Feng, Q. Y., Liu, Q., Li, H. Y., Song, X. L., Hu, Z. X., Xu, Z. Y., Li, J. H., Li, M. J., Zheng, W. L., Li, Z. J., & Pan, H. W. (2020). Posttranscriptional regulation of MMP-9 by HuR contributes to IL-1β-induced pterygium fibroblast migration and invasion. Journal of Cellular Physiology, 235(6), 5130-5140. https://doi.org/10.1002/jcp.29387
Cui YH, et al. Posttranscriptional Regulation of MMP-9 By HuR Contributes to IL-1β-induced Pterygium Fibroblast Migration and Invasion. J Cell Physiol. 2020;235(6):5130-5140. PubMed PMID: 31691974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Posttranscriptional regulation of MMP-9 by HuR contributes to IL-1β-induced pterygium fibroblast migration and invasion. AU - Cui,Yu-Hong, AU - Feng,Qing-Yang, AU - Liu,Qun, AU - Li,Hong-Yang, AU - Song,Xi-Ling, AU - Hu,Zi-Xuan, AU - Xu,Zhi-Yi, AU - Li,Jia-Hui, AU - Li,Mei-Jun, AU - Zheng,Wen-Lin, AU - Li,Zhi-Jie, AU - Pan,Hong-Wei, Y1 - 2019/11/06/ PY - 2019/01/18/received PY - 2019/09/30/accepted PY - 2019/11/7/pubmed PY - 2019/11/7/medline PY - 2019/11/7/entrez KW - HuR KW - fibroblast KW - inflammation KW - migration KW - pterygium SP - 5130 EP - 5140 JF - Journal of cellular physiology JO - J Cell Physiol VL - 235 IS - 6 N2 - Inflammation is considered to be critical in the pterygium progression and recurrence. However, the underlying molecular mechanism is not well understood. Herein, we investigated the potential role of RNA binding protein human antigen R (HuR) responsible for the impact of inflammation on pterygium development. The expression of HuR and matrix metallopeptidase-9 (MMP-9) in pterygium and normal conjunctiva was detected with immunohistochemistry and quantitative reverse transcription polymerase chain reaction (qRT-PCR). The influence of interleukin-1β (IL-1β) on HuR expression and cellular distribution was determined with western blot and immunofluorescence. The pterygium fibroblast (PTF) migration was determined with scratch wound healing assay and Transwell migration assay. MMP-9 production was determined with qRT-PCR and gelatin zymography. The interaction between HuR and MMP-9 was investigated with RNP immunoprecipitation (IP) followed by RT-PCR and messenger RNA (mRNA) stability analysis. HuR and MMP-9 expression are elevated in pterygium, especially progressive pterygium compared with normal conjunctiva. IL-1β could increase the expression and nucleus-cytoplasm shuttle of HuR in cultured PTFs. HuR mediated the stimulatory effect of IL-1β on PTF migration and MMP-9 production. HuR bound to MMP-9 mRNA and in turn increased it stability. Our results suggest that posttranscriptional regulation of MMP-9 via stabilizing mRNA by HuR might contribute to the stimulatory effect of inflammatory factor IL-1β on pterygium progression. These findings shed light on the pathogenesis of pterygium and provide a promising target for adjuvant treatment of pterygium. SN - 1097-4652 UR - https://www.unboundmedicine.com/medline/citation/31691974/Posttranscriptional_regulation_of_MMP-9_by_HuR_contributes_to_IL-1β-induced_pterygium_fibroblast_migration_and_invasion L2 - https://doi.org/10.1002/jcp.29387 DB - PRIME DP - Unbound Medicine ER -
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