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Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity.
Int J Nanomedicine. 2019; 14:6679-6690.IJ

Abstract

BACKGROUND

The synthesis of silver nanoparticles (AgNPs) using food waste materials and their biomedical applications have garnered considerable attention recently.

METHODS

Here, we investigated the synthesis of AgNPs using an aqueous extract of outer peel of Pisum sativum under different lighting conditions using standard procedures and explored their antidiabetic, cytotoxicity, antioxidant, and antibacterial potential.

RESULTS

Characterization of AgNPs was done by Ultra Violet (UV-VIS) spectroscopy that showed absorption maxima at 456 nm for the samples exposed to laboratory lighting and at 464 nm for the samples exposed to direct sunlight, by scanning electron microscopy and energy-dispersive X-ray analysis that showed the surface nature and their elemental composition with a strong peak at 3 keV that corresponded to Ag (61.85 wt%), by Fourier-transform infrared spectroscopy that predicted the functional groups involved, and by X-ray powder diffraction that showed the structural properties. The average diameter of the synthesized AgNPs was calculated to be in the range of 10-25 nm. AgNPs exhibited promising antidiabetic activity as determined by inhibition of α-glucosidase (95.29% inhibition at 10 µg/mL and IC50 value of 2.10 µg/mL) and cytotoxicity (IC50 value 4.0 µg/mL as calculated from the slope equation) against HepG2 cells. Furthermore, they also exhibited moderate antioxidant activity (50.17% reduction of 1,1-diphenyl-2-picrylhydrazyl at 100 µg/mL) and antibacterial activity against four human pathogenic bacteria (as indicated by 8.70-11.10 mm inhibition zones on agar plates).

CONCLUSION

In conclusion, the results confirm that food waste can be used in the synthesis of AgNPs and that the latter have the potential for applications in various fields including diabetic and cancer treatments as well as in biomedicine for the manufacture of antibacterial coatings in medical devices and instruments.

Authors+Show Affiliations

Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea.Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea.Department of Food Science & Biotechnology, Dongguk University-Seoul, Goyang 10326, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31695363

Citation

Patra, Jayanta Kumar, et al. "Facile Green Biosynthesis of Silver Nanoparticles Using Pisum Sativum L. Outer Peel Aqueous Extract and Its Antidiabetic, Cytotoxicity, Antioxidant, and Antibacterial Activity." International Journal of Nanomedicine, vol. 14, 2019, pp. 6679-6690.
Patra JK, Das G, Shin HS. Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity. Int J Nanomedicine. 2019;14:6679-6690.
Patra, J. K., Das, G., & Shin, H. S. (2019). Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity. International Journal of Nanomedicine, 14, 6679-6690. https://doi.org/10.2147/IJN.S212614
Patra JK, Das G, Shin HS. Facile Green Biosynthesis of Silver Nanoparticles Using Pisum Sativum L. Outer Peel Aqueous Extract and Its Antidiabetic, Cytotoxicity, Antioxidant, and Antibacterial Activity. Int J Nanomedicine. 2019;14:6679-6690. PubMed PMID: 31695363.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity. AU - Patra,Jayanta Kumar, AU - Das,Gitishree, AU - Shin,Han-Seung, Y1 - 2019/08/19/ PY - 2019/04/17/received PY - 2019/07/12/accepted PY - 2019/11/8/entrez PY - 2019/11/7/pubmed PY - 2020/1/7/medline KW - Pisum sativum KW - antibacterial KW - antidiabetic KW - antioxidant KW - cytotoxicity KW - garden pea KW - silver nanoparticles SP - 6679 EP - 6690 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 14 N2 - BACKGROUND: The synthesis of silver nanoparticles (AgNPs) using food waste materials and their biomedical applications have garnered considerable attention recently. METHODS: Here, we investigated the synthesis of AgNPs using an aqueous extract of outer peel of Pisum sativum under different lighting conditions using standard procedures and explored their antidiabetic, cytotoxicity, antioxidant, and antibacterial potential. RESULTS: Characterization of AgNPs was done by Ultra Violet (UV-VIS) spectroscopy that showed absorption maxima at 456 nm for the samples exposed to laboratory lighting and at 464 nm for the samples exposed to direct sunlight, by scanning electron microscopy and energy-dispersive X-ray analysis that showed the surface nature and their elemental composition with a strong peak at 3 keV that corresponded to Ag (61.85 wt%), by Fourier-transform infrared spectroscopy that predicted the functional groups involved, and by X-ray powder diffraction that showed the structural properties. The average diameter of the synthesized AgNPs was calculated to be in the range of 10-25 nm. AgNPs exhibited promising antidiabetic activity as determined by inhibition of α-glucosidase (95.29% inhibition at 10 µg/mL and IC50 value of 2.10 µg/mL) and cytotoxicity (IC50 value 4.0 µg/mL as calculated from the slope equation) against HepG2 cells. Furthermore, they also exhibited moderate antioxidant activity (50.17% reduction of 1,1-diphenyl-2-picrylhydrazyl at 100 µg/mL) and antibacterial activity against four human pathogenic bacteria (as indicated by 8.70-11.10 mm inhibition zones on agar plates). CONCLUSION: In conclusion, the results confirm that food waste can be used in the synthesis of AgNPs and that the latter have the potential for applications in various fields including diabetic and cancer treatments as well as in biomedicine for the manufacture of antibacterial coatings in medical devices and instruments. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/31695363/Facile_green_biosynthesis_of_silver_nanoparticles_using_Pisum_sativum_L__outer_peel_aqueous_extract_and_its_antidiabetic_cytotoxicity_antioxidant_and_antibacterial_activity_ L2 - https://dx.doi.org/10.2147/IJN.S212614 DB - PRIME DP - Unbound Medicine ER -