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A novel rat model for tracheal mucosal damage assessment of following long term intubation.

Abstract

OBJECTIVE

Tracheal mucosal damage is a well-known complication of endo-tracheal intubation and animal models are essential for studying the underlying cellular injury cascade. The novel rat model described here is based on retrograde intubation via tracheotomy and suture fixation of the tube. It aims to simulate the common clinical scenario of tube-related airway damage due to long term intubation.

STUDY DESIGN

Prospective randomized control pilot study.

METHODS

Male Sprague-Dawley were randomly assigned into two groups: control (no intubation, n = 10), one week of intubation (n = 13). The animals were then euthanized and the trachea was sent for histological analysis. Epithelial damage, mucosal thickness, mucosal gland hypertrophy and fibrosis were reviewed.

RESULTS

Intubation procedure survival rate was 84.6% (11/13) and 100% in the control (10/10). The damaged ciliary mechanism was a common finding in the intubated group compared to the preserved normal ciliary architecture in almost all control rats. Average tracheal mucosal thickness was 119.0 ± 21.8 μm for the control group and 254.6 ± 22.8 μm for the intubated group, (p < 0.001). The ciliary damage score was 1.00 ± 0.02 in the intubated group, and 0 ± 0.02 in the control group. (p < 0.001). The (objective) average total tracheal mucosal gland area was 19,530 ± 24,606 in the intubated group and 10,031 ± 23,461 in the control group (p < 0,05). Collagen deposition seems higher in the intubated trachea compared to the control.

CONCLUSIONS

We describe a novel rat-based animal model for simulating tracheal mucosal damage following long term intubation. This animal model is easy to carry out, reproducible and involves containable animal mortality rates.

LEVEL OF EVIDENCE

I.

Authors+Show Affiliations

Department of Otolaryngology, Galilee Medical Center, Nahariya, Israel.Cardiac Research Laboratory, Galilee Medical Center, Nahariya, Israel.Department of Otolaryngology, Galilee Medical Center, Nahariya, Israel.Department of Otolaryngology, Galilee Medical Center, Nahariya, Israel.Department of Otolaryngology, Galilee Medical Center, Nahariya, Israel; The Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel.Cardiac Research Laboratory, Galilee Medical Center, Nahariya, Israel; The Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel.Department of Pharmaceutical Engineering, Azrieli College of Engineering, Jerusalem, Israel.Department of Otolaryngology, Galilee Medical Center, Nahariya, Israel; The Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel. Electronic address: maayang@gmc.gov.il.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31698244

Citation

Jahshan, F, et al. "A Novel Rat Model for Tracheal Mucosal Damage Assessment of Following Long Term Intubation." International Journal of Pediatric Otorhinolaryngology, vol. 128, 2019, p. 109738.
Jahshan F, Ertracht O, Eisenbach N, et al. A novel rat model for tracheal mucosal damage assessment of following long term intubation. Int J Pediatr Otorhinolaryngol. 2019;128:109738.
Jahshan, F., Ertracht, O., Eisenbach, N., Daoud, A., Sela, E., Atar, S., ... Gruber, M. (2019). A novel rat model for tracheal mucosal damage assessment of following long term intubation. International Journal of Pediatric Otorhinolaryngology, 128, p. 109738. doi:10.1016/j.ijporl.2019.109738.
Jahshan F, et al. A Novel Rat Model for Tracheal Mucosal Damage Assessment of Following Long Term Intubation. Int J Pediatr Otorhinolaryngol. 2019 Oct 24;128:109738. PubMed PMID: 31698244.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel rat model for tracheal mucosal damage assessment of following long term intubation. AU - Jahshan,F, AU - Ertracht,O, AU - Eisenbach,N, AU - Daoud,A, AU - Sela,E, AU - Atar,S, AU - Abu Ammar,A, AU - Gruber,M, Y1 - 2019/10/24/ PY - 2019/07/08/received PY - 2019/10/18/revised PY - 2019/10/21/accepted PY - 2019/11/8/pubmed PY - 2019/11/8/medline PY - 2019/11/8/entrez KW - Airway KW - Animal model KW - Intubation KW - Long term intubation KW - Trachea SP - 109738 EP - 109738 JF - International journal of pediatric otorhinolaryngology JO - Int. J. Pediatr. Otorhinolaryngol. VL - 128 N2 - OBJECTIVE: Tracheal mucosal damage is a well-known complication of endo-tracheal intubation and animal models are essential for studying the underlying cellular injury cascade. The novel rat model described here is based on retrograde intubation via tracheotomy and suture fixation of the tube. It aims to simulate the common clinical scenario of tube-related airway damage due to long term intubation. STUDY DESIGN: Prospective randomized control pilot study. METHODS: Male Sprague-Dawley were randomly assigned into two groups: control (no intubation, n = 10), one week of intubation (n = 13). The animals were then euthanized and the trachea was sent for histological analysis. Epithelial damage, mucosal thickness, mucosal gland hypertrophy and fibrosis were reviewed. RESULTS: Intubation procedure survival rate was 84.6% (11/13) and 100% in the control (10/10). The damaged ciliary mechanism was a common finding in the intubated group compared to the preserved normal ciliary architecture in almost all control rats. Average tracheal mucosal thickness was 119.0 ± 21.8 μm for the control group and 254.6 ± 22.8 μm for the intubated group, (p < 0.001). The ciliary damage score was 1.00 ± 0.02 in the intubated group, and 0 ± 0.02 in the control group. (p < 0.001). The (objective) average total tracheal mucosal gland area was 19,530 ± 24,606 in the intubated group and 10,031 ± 23,461 in the control group (p < 0,05). Collagen deposition seems higher in the intubated trachea compared to the control. CONCLUSIONS: We describe a novel rat-based animal model for simulating tracheal mucosal damage following long term intubation. This animal model is easy to carry out, reproducible and involves containable animal mortality rates. LEVEL OF EVIDENCE: I. SN - 1872-8464 UR - https://www.unboundmedicine.com/medline/citation/31698244/A_novel_rat_model_for_tracheal_mucosal_damage_assessment_of_following_long_term_intubation L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-5876(19)30491-4 DB - PRIME DP - Unbound Medicine ER -