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The Effect of PEGDE Concentration and Temperature on Physicochemical and Biological Properties of Chitosan.
Polymers (Basel) 2019; 11(11)P

Abstract

Chitosan (CHT) is a polysaccharide with multiple claimed properties and outstanding biocompatibility, generally attributed to the presence of protonable amino groups rendering a cationic natural polymer. However, the effect of changes in CHT structure due to hydration is not considered in its performance. This study compares the effects on biocompatibility after drying at 25 °C and 150 °C scaffolds of chitosan, polyethylene glycol diglycidyl ether (PEGDE) crosslinked CHT (low, medium and high concentration) and glutaraldehyde (GA) crosslinked CHT. PEGDE crosslinked CHT showed a reduction in free amino groups and the amide I/II ratio, which exhaustive drying reduced further. In X-ray diffraction (DRX) analysis, PEGDE crosslinked CHT showed multiple peaks, whereas the crystallinity percentage was reduced with an increase in PEGDE concentration and thermal treatments at 150 °C. In a direct contact cell assay, high osteoblast viability was achieved at low and medium PEDGE concentrations, which was improved when the crosslinked scaffolds were thermally treated at 150 °C. This was attributed to its partial hydrophilicity, low crystallinity and low surface roughness; this in spite of the small reduction in the amount of free amino groups on the surface induced during drying at 150 °C. Furthermore, PEGDE crosslinked CHT scaffolds showed strong vinculin and integrin 1β expression, which render them suitable for bone contact applications.

Authors+Show Affiliations

Unidad de Materiales, Centro de Investigación Científica de Yucatán, México. Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, C.P. 97205 Mérida, Yucatán, Mexico.Unidad de Materiales, Centro de Investigación Científica de Yucatán, México. Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, C.P. 97205 Mérida, Yucatán, Mexico.Unidad de Materiales, Centro de Investigación Científica de Yucatán, México. Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, C.P. 97205 Mérida, Yucatán, Mexico.Unidad de Materiales, Centro de Investigación Científica de Yucatán, México. Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, C.P. 97205 Mérida, Yucatán, Mexico.Laboratorio de Ciencias Básicas, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, México. Ave. Dr. Manuel Nava No. 2, Zona Universitaria, C.P. 78290 San Luis, S.L.P., Mexico.Laboratorio de Ciencias Básicas, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, México. Ave. Dr. Manuel Nava No. 2, Zona Universitaria, C.P. 78290 San Luis, S.L.P., Mexico.Instituto de Ciencia y Tecnología de Polímeros. España. Calle Juan de la Cierva, 3, C.P 28006 Madrid, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31703343

Citation

Chuc-Gamboa, Martha Gabriela, et al. "The Effect of PEGDE Concentration and Temperature On Physicochemical and Biological Properties of Chitosan." Polymers, vol. 11, no. 11, 2019.
Chuc-Gamboa MG, Vargas-Coronado RF, Cervantes-Uc JM, et al. The Effect of PEGDE Concentration and Temperature on Physicochemical and Biological Properties of Chitosan. Polymers (Basel). 2019;11(11).
Chuc-Gamboa, M. G., Vargas-Coronado, R. F., Cervantes-Uc, J. M., Cauich-Rodríguez, J. V., Escobar-García, D. M., Pozos-Guillén, A., & San Román Del Barrio, J. (2019). The Effect of PEGDE Concentration and Temperature on Physicochemical and Biological Properties of Chitosan. Polymers, 11(11), doi:10.3390/polym11111830.
Chuc-Gamboa MG, et al. The Effect of PEGDE Concentration and Temperature On Physicochemical and Biological Properties of Chitosan. Polymers (Basel). 2019 Nov 7;11(11) PubMed PMID: 31703343.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Effect of PEGDE Concentration and Temperature on Physicochemical and Biological Properties of Chitosan. AU - Chuc-Gamboa,Martha Gabriela, AU - Vargas-Coronado,Rossana Faride, AU - Cervantes-Uc,José Manuel, AU - Cauich-Rodríguez,Juan Valerio, AU - Escobar-García,Diana María, AU - Pozos-Guillén,Amaury, AU - San Román Del Barrio,Julio, Y1 - 2019/11/07/ PY - 2019/09/30/received PY - 2019/10/25/revised PY - 2019/10/28/accepted PY - 2019/11/10/entrez KW - PEGDE KW - biocompatibility KW - chitosan KW - crosslinked KW - drying temperature KW - glutaraldehyde KW - osteoblasts KW - scaffolds JF - Polymers JO - Polymers (Basel) VL - 11 IS - 11 N2 - Chitosan (CHT) is a polysaccharide with multiple claimed properties and outstanding biocompatibility, generally attributed to the presence of protonable amino groups rendering a cationic natural polymer. However, the effect of changes in CHT structure due to hydration is not considered in its performance. This study compares the effects on biocompatibility after drying at 25 °C and 150 °C scaffolds of chitosan, polyethylene glycol diglycidyl ether (PEGDE) crosslinked CHT (low, medium and high concentration) and glutaraldehyde (GA) crosslinked CHT. PEGDE crosslinked CHT showed a reduction in free amino groups and the amide I/II ratio, which exhaustive drying reduced further. In X-ray diffraction (DRX) analysis, PEGDE crosslinked CHT showed multiple peaks, whereas the crystallinity percentage was reduced with an increase in PEGDE concentration and thermal treatments at 150 °C. In a direct contact cell assay, high osteoblast viability was achieved at low and medium PEDGE concentrations, which was improved when the crosslinked scaffolds were thermally treated at 150 °C. This was attributed to its partial hydrophilicity, low crystallinity and low surface roughness; this in spite of the small reduction in the amount of free amino groups on the surface induced during drying at 150 °C. Furthermore, PEGDE crosslinked CHT scaffolds showed strong vinculin and integrin 1β expression, which render them suitable for bone contact applications. SN - 2073-4360 UR - https://www.unboundmedicine.com/medline/citation/31703343/The_Effect_of_PEGDE_Concentration_and_Temperature_on_Physicochemical_and_Biological_Properties_of_Chitosan DB - PRIME DP - Unbound Medicine ER -