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Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels.
Sci Rep. 2019 11 08; 9(1):16292.SR

Abstract

MERS-CoV seronegative and seropositive camels received a single intramuscular dose of ChAdOx1 MERS, a replication-deficient adenoviral vectored vaccine expressing MERS-CoV spike protein, with further groups receiving control vaccinations. Infectious camels with active naturally acquired MERS-CoV infection, were co-housed with the vaccinated camels at a ratio of 1:2 (infected:vaccinated); nasal discharge and virus titres were monitored for 14 days. Overall, the vaccination reduced virus shedding and nasal discharge (p = 0.0059 and p = 0.0274, respectively). Antibody responses in seropositive camels were enhancedby the vaccine; these camels had a higher average age than seronegative. Older seronegative camels responded more strongly to vaccination than younger animals; and neutralising antibodies were detected in nasal swabs. Further work is required to optimise vaccine regimens for younger seronegative camels.

Authors+Show Affiliations

Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia. harbina2@ngha.med.sa. King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia. harbina2@ngha.med.sa.Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia.Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia.Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia.Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia.Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia.Department of Bioinformatics and Biostatistics, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia.Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. Vaccines and Immunotherapy Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia. Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, El Geish Street, 33516, Egypt.Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia.College of Agriculture and Veterinary Medicine, Qassim University, Qassim, Saudi Arabia.Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia.Viral Pseudotype Unit, Medway School of Pharmacy, University of Kent, Chatham, Kent, ME4 4TB, United Kingdom.Institute of Virology, Philipps University of Marburg, Marburg, Germany. German Center for Infection Research (DZIF), Partner Site Gieβen-Marburg-Langen, Germany.Institute of Virology, Philipps University of Marburg, Marburg, Germany. German Center for Infection Research (DZIF), Partner Site Gieβen-Marburg-Langen, Germany.Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia.Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia.International Vaccine Institute, Seoul, South Korea.Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia. Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.The Jenner Institute, University of Oxford, Oxford, OX3 7DQ, UK.Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia.Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia.Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia.The Jenner Institute, University of Oxford, Oxford, OX3 7DQ, UK.Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia. King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia. Department of Infection Prevention and Control, Ministry of National Guard - Health Affairs, Riyadh, Saudi Arabia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31705137

Citation

Alharbi, Naif Khalaf, et al. "Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels." Scientific Reports, vol. 9, no. 1, 2019, p. 16292.
Alharbi NK, Qasim I, Almasoud A, et al. Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels. Sci Rep. 2019;9(1):16292.
Alharbi, N. K., Qasim, I., Almasoud, A., Aljami, H. A., Alenazi, M. W., Alhafufi, A., Aldibasi, O. S., Hashem, A. M., Kasem, S., Albrahim, R., Aldubaib, M., Almansour, A., Temperton, N. J., Kupke, A., Becker, S., Abu-Obaidah, A., Alkarar, A., Yoon, I. K., Azhar, E., ... Balkhy, H. H. (2019). Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels. Scientific Reports, 9(1), 16292. https://doi.org/10.1038/s41598-019-52730-4
Alharbi NK, et al. Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels. Sci Rep. 2019 11 8;9(1):16292. PubMed PMID: 31705137.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels. AU - Alharbi,Naif Khalaf, AU - Qasim,Ibrahim, AU - Almasoud,Abdulrahman, AU - Aljami,Haya A, AU - Alenazi,Mohamed W, AU - Alhafufi,Ali, AU - Aldibasi,Omar S, AU - Hashem,Anwar M, AU - Kasem,Samy, AU - Albrahim,Raed, AU - Aldubaib,Musaad, AU - Almansour,Ali, AU - Temperton,Nigel J, AU - Kupke,Alexandra, AU - Becker,Stephan, AU - Abu-Obaidah,Ali, AU - Alkarar,Ali, AU - Yoon,In-Kyu, AU - Azhar,Esam, AU - Lambe,Teresa, AU - Bayoumi,Faisal, AU - Aldowerij,Ali, AU - Ibrahim,Osman H, AU - Gilbert,Sarah C, AU - Balkhy,Hanan H, Y1 - 2019/11/08/ PY - 2019/07/22/received PY - 2019/10/16/accepted PY - 2019/11/10/entrez PY - 2019/11/11/pubmed PY - 2020/9/12/medline SP - 16292 EP - 16292 JF - Scientific reports JO - Sci Rep VL - 9 IS - 1 N2 - MERS-CoV seronegative and seropositive camels received a single intramuscular dose of ChAdOx1 MERS, a replication-deficient adenoviral vectored vaccine expressing MERS-CoV spike protein, with further groups receiving control vaccinations. Infectious camels with active naturally acquired MERS-CoV infection, were co-housed with the vaccinated camels at a ratio of 1:2 (infected:vaccinated); nasal discharge and virus titres were monitored for 14 days. Overall, the vaccination reduced virus shedding and nasal discharge (p = 0.0059 and p = 0.0274, respectively). Antibody responses in seropositive camels were enhancedby the vaccine; these camels had a higher average age than seronegative. Older seronegative camels responded more strongly to vaccination than younger animals; and neutralising antibodies were detected in nasal swabs. Further work is required to optimise vaccine regimens for younger seronegative camels. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/31705137/Humoral_Immunogenicity_and_Efficacy_of_a_Single_Dose_of_ChAdOx1_MERS_Vaccine_Candidate_in_Dromedary_Camels_ L2 - https://doi.org/10.1038/s41598-019-52730-4 DB - PRIME DP - Unbound Medicine ER -