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Biosynthesis of silver and gold nanoparticles using Musa acuminata colla flower and its pharmaceutical activity against bacteria and anticancer efficacy.
J Photochem Photobiol B. 2019 Dec; 201:111670.JP

Abstract

Synthesis of nanoparticles using plant sources as reducing agent has become important, as physical and chemical methods are costlier and affects environment. Hence it is important to develop environment friendly nanoparticle synthesis by avoiding the use of toxic chemicals. The present study aimed to synthesize silver nanoparticles (Ag Nps) and gold nanoparticles (AuNps) using Musa acuminata colla flower and its pharmaceutical activity against extended spectrum beta-lactamase (ESBL) gene producing bacteria and anticancer efficacy. The synthesized Ag and Au NPs were analysed by means of UV-Vis, FTIR, XRD,SEM and EDAX evidenced the bioreduction of Ag+ ions to Ag0 and Au3+ ions to Au0 respectively. Both nanoparticles and flower extracts were studied for antibacterial activity of ESBL gene producing bacteria by disc diffusion and microdilution (Resazurin) method. In vitro anticancer efficacy (MCF-7) and toxicity (VERO) of AgNPs, AuNPs, aqueous extract and ethanol extract of flowers were performed by MTT assay. IC50 value for DPPH analysis was at 390 μg and 460 μg for ethanol and aqueous extract respectively. Total antioxidant content was found be 740 μg/mg and 460 μg/mg for ethanol and aqueous extract. GCMS analysis authenticated the existence of the compounds namely, 9,12-octadecadienoic acid(z,z)- and n-hexadecanoic acid in the crude extract of the samples. Among the samples, AgNPs had best antibacterial activity. AgNPs and AuNPs were confirmed by colour change to reddish brown and ruby red. Further ƛmax were obtained at 474 and 540 nm by UV - visible spectrum. SEM analysis revealed the particle size ranges from 12.6 to 15.7 nm for silver and 10.1 to 15.6 nm for gold nanoparticles. The EDAX spectrum shows a strong signal for elemental Ag and Au at ~ 3 keV and 1.5 keV. The XRD patterns for silver and gold nanoparticles at 36.701, 42.900, 63.281 and 76.398 corresponding to the lattice planes 2.4467, 2.1064, 1.46839, 1.24564 nm and 27.32, 36.7228, 39.56, 42.888, 63.253, 63.253, 65.02 and 76.383 corresponding to the lattice planes 3.262, 2.44530, 2.276, 2.1070, 1.46897, 1.4332 and 1.24585 nm. The IC50 values for MCF-7 and VERO cells were 30.0 μg/ml and 55.0 μg/ml respectively.

Authors+Show Affiliations

Department of Plant Biology & Biotechnology, Loyola College (Affiliated to Madras University), Chennai 600 034, Tamil Nadu, India.Department of Plant Biology & Biotechnology, Loyola College (Affiliated to Madras University), Chennai 600 034, Tamil Nadu, India. Electronic address: agastian@loyolacollege.edu.Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31706087

Citation

Valsalam, Saritha, et al. "Biosynthesis of Silver and Gold Nanoparticles Using Musa Acuminata Colla Flower and Its Pharmaceutical Activity Against Bacteria and Anticancer Efficacy." Journal of Photochemistry and Photobiology. B, Biology, vol. 201, 2019, p. 111670.
Valsalam S, Agastian P, Esmail GA, et al. Biosynthesis of silver and gold nanoparticles using Musa acuminata colla flower and its pharmaceutical activity against bacteria and anticancer efficacy. J Photochem Photobiol B. 2019;201:111670.
Valsalam, S., Agastian, P., Esmail, G. A., Ghilan, A. M., Al-Dhabi, N. A., & Arasu, M. V. (2019). Biosynthesis of silver and gold nanoparticles using Musa acuminata colla flower and its pharmaceutical activity against bacteria and anticancer efficacy. Journal of Photochemistry and Photobiology. B, Biology, 201, 111670. https://doi.org/10.1016/j.jphotobiol.2019.111670
Valsalam S, et al. Biosynthesis of Silver and Gold Nanoparticles Using Musa Acuminata Colla Flower and Its Pharmaceutical Activity Against Bacteria and Anticancer Efficacy. J Photochem Photobiol B. 2019;201:111670. PubMed PMID: 31706087.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biosynthesis of silver and gold nanoparticles using Musa acuminata colla flower and its pharmaceutical activity against bacteria and anticancer efficacy. AU - Valsalam,Saritha, AU - Agastian,Paul, AU - Esmail,Galal Ali, AU - Ghilan,Abdul-Kareem Mohammed, AU - Al-Dhabi,Naif Abdullah, AU - Arasu,Mariadhas Valan, Y1 - 2019/10/31/ PY - 2019/09/26/received PY - 2019/10/20/revised PY - 2019/10/23/accepted PY - 2019/11/11/pubmed PY - 2019/11/30/medline PY - 2019/11/10/entrez KW - Anticancer KW - FTIR KW - GC–MS KW - Gold nanoparticles KW - Musa acuminata colla KW - Silver KW - UV - visible spectrum KW - XRD SP - 111670 EP - 111670 JF - Journal of photochemistry and photobiology. B, Biology JO - J Photochem Photobiol B VL - 201 N2 - Synthesis of nanoparticles using plant sources as reducing agent has become important, as physical and chemical methods are costlier and affects environment. Hence it is important to develop environment friendly nanoparticle synthesis by avoiding the use of toxic chemicals. The present study aimed to synthesize silver nanoparticles (Ag Nps) and gold nanoparticles (AuNps) using Musa acuminata colla flower and its pharmaceutical activity against extended spectrum beta-lactamase (ESBL) gene producing bacteria and anticancer efficacy. The synthesized Ag and Au NPs were analysed by means of UV-Vis, FTIR, XRD,SEM and EDAX evidenced the bioreduction of Ag+ ions to Ag0 and Au3+ ions to Au0 respectively. Both nanoparticles and flower extracts were studied for antibacterial activity of ESBL gene producing bacteria by disc diffusion and microdilution (Resazurin) method. In vitro anticancer efficacy (MCF-7) and toxicity (VERO) of AgNPs, AuNPs, aqueous extract and ethanol extract of flowers were performed by MTT assay. IC50 value for DPPH analysis was at 390 μg and 460 μg for ethanol and aqueous extract respectively. Total antioxidant content was found be 740 μg/mg and 460 μg/mg for ethanol and aqueous extract. GCMS analysis authenticated the existence of the compounds namely, 9,12-octadecadienoic acid(z,z)- and n-hexadecanoic acid in the crude extract of the samples. Among the samples, AgNPs had best antibacterial activity. AgNPs and AuNPs were confirmed by colour change to reddish brown and ruby red. Further ƛmax were obtained at 474 and 540 nm by UV - visible spectrum. SEM analysis revealed the particle size ranges from 12.6 to 15.7 nm for silver and 10.1 to 15.6 nm for gold nanoparticles. The EDAX spectrum shows a strong signal for elemental Ag and Au at ~ 3 keV and 1.5 keV. The XRD patterns for silver and gold nanoparticles at 36.701, 42.900, 63.281 and 76.398 corresponding to the lattice planes 2.4467, 2.1064, 1.46839, 1.24564 nm and 27.32, 36.7228, 39.56, 42.888, 63.253, 63.253, 65.02 and 76.383 corresponding to the lattice planes 3.262, 2.44530, 2.276, 2.1070, 1.46897, 1.4332 and 1.24585 nm. The IC50 values for MCF-7 and VERO cells were 30.0 μg/ml and 55.0 μg/ml respectively. SN - 1873-2682 UR - https://www.unboundmedicine.com/medline/citation/31706087/Biosynthesis_of_silver_and_gold_nanoparticles_using_Musa_acuminata_colla_flower_and_its_pharmaceutical_activity_against_bacteria_and_anticancer_efficacy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1011-1344(19)31293-X DB - PRIME DP - Unbound Medicine ER -